Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,a...Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection.WB test was used to measure the levels of cyclinD1,p27 and FOXO3.Meanwhile.QPCR was used to detect miR-184expression in glioma cell line.glioma tissues and adjacent tissues.Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3.Results:QPCR results showed a significaat lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue.MTT and plate cloning experiments have shown that after overexpressing of miR-184,the cell proliferation capacity of glioma U87 and T98G was signifieantly increased,which was significantly inhibited after the inhibition of miN-184.WB results showed a lower expression level of p27 in U87 and T98G cells,and a higher expression level of cyclind1after over-expressing of miR-184 wss observed.However.a lower expression level of eyelinD1and a higher expression level of p27 after the inhibition of miR-184.The luciferase activity was inhibited after the over-expressing of miR-184.Conclusions:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein.It plays an important role in the occurrence and development of gliomas.展开更多
目的:探讨胃癌中与雌激素受体(estrogen receptor,ER)-α36有关的miRNA表达差异.方法:构建稳定转染低表达ER-α36和高表达ER-α36的SGC7901细胞系,进行miRNA测序;对20例人体胃癌组织行ER-α36免疫组织化学染色,将其分为阳性组和阴性组...目的:探讨胃癌中与雌激素受体(estrogen receptor,ER)-α36有关的miRNA表达差异.方法:构建稳定转染低表达ER-α36和高表达ER-α36的SGC7901细胞系,进行miRNA测序;对20例人体胃癌组织行ER-α36免疫组织化学染色,将其分为阳性组和阴性组后通过Realtime PCR检测miR-184和miR-143的表达.结果:与对照组SGC7901细胞相比,高表达ER-α36的SGC7901细胞出现miR-184、m i R-143等m i R N A表达下调(m i R-184下调2.78倍;miR-143下调2.95倍),低表达ER-α36的SGC7901细胞反之(miR-184上调3.17倍;miR-143上调3.11倍).PCR结果显示人体胃癌组织中ER-α36阴性表达组与ER-α36阳性表达组相比,miR-184上调14.2倍,miR-143上调9.67倍.结论:miR-184和miR-143可能是调控ER-α36的重要分子,参与介导胃癌的发生与发展.展开更多
基金supported by National Science Foundation(Grant No.81372721)
文摘Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection.WB test was used to measure the levels of cyclinD1,p27 and FOXO3.Meanwhile.QPCR was used to detect miR-184expression in glioma cell line.glioma tissues and adjacent tissues.Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3.Results:QPCR results showed a significaat lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue.MTT and plate cloning experiments have shown that after overexpressing of miR-184,the cell proliferation capacity of glioma U87 and T98G was signifieantly increased,which was significantly inhibited after the inhibition of miN-184.WB results showed a lower expression level of p27 in U87 and T98G cells,and a higher expression level of cyclind1after over-expressing of miR-184 wss observed.However.a lower expression level of eyelinD1and a higher expression level of p27 after the inhibition of miR-184.The luciferase activity was inhibited after the over-expressing of miR-184.Conclusions:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein.It plays an important role in the occurrence and development of gliomas.
文摘目的:探讨胃癌中与雌激素受体(estrogen receptor,ER)-α36有关的miRNA表达差异.方法:构建稳定转染低表达ER-α36和高表达ER-α36的SGC7901细胞系,进行miRNA测序;对20例人体胃癌组织行ER-α36免疫组织化学染色,将其分为阳性组和阴性组后通过Realtime PCR检测miR-184和miR-143的表达.结果:与对照组SGC7901细胞相比,高表达ER-α36的SGC7901细胞出现miR-184、m i R-143等m i R N A表达下调(m i R-184下调2.78倍;miR-143下调2.95倍),低表达ER-α36的SGC7901细胞反之(miR-184上调3.17倍;miR-143上调3.11倍).PCR结果显示人体胃癌组织中ER-α36阴性表达组与ER-α36阳性表达组相比,miR-184上调14.2倍,miR-143上调9.67倍.结论:miR-184和miR-143可能是调控ER-α36的重要分子,参与介导胃癌的发生与发展.
