Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35 lun...Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35 lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretion and gene expression.Tetra-Methylpyrazine (TTMP),extracted from a traditional Chinese medicinal herb which has been used in clinic to reverse the Th2 status of cancer patients in China,was added to PBMC culture. Determined by RT-PCR,the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and 11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4,60% for IL-6 and 80% for 1L-10) in patients' PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in the patients for type 1 cytokines was also in a low level (0.111 for IFN-γ,0.119 for IL-2) in comparison with a relative high level for type 2 cytokines (0.319 for IL-4,0.303 for IL-6 and 0.377 for IL-10).Meanwhile,both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factor T-bet (31.4% and 0.142,respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378, respectively).The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantly when compared with healthy control.In contrast,the levels IL-4 and IL-6 in patients were significantly higher than those in healthy subjects by ELISA analysis.TTMP could enhance supernatant concentration and gene expression levels of IFN-γ,IL-2 and T-bet,but reduced those of type 2 cytokines.These results demonstrate that the lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2 dominant status,which might offer an alternative therapeutic regime for lung cancer patients.Cellular & Molecular Immunology.2004;1(1):63-70.展开更多
The 2-methylpyrazine was synthesized by catalytic reaction of ethylene diamine and propylene glycol at 380 ℃. The alumina supported copper catalysts with promoter were prepared by impregnation method, characterized b...The 2-methylpyrazine was synthesized by catalytic reaction of ethylene diamine and propylene glycol at 380 ℃. The alumina supported copper catalysts with promoter were prepared by impregnation method, characterized by ICP-AES, BET and TPR. The results demonstrated that the dehydrogenation was improved by addition of chromium promoter. The selectivity of 2-methylpyrazine reached 84.75%, while the conversions of reactants were also enhanced.展开更多
A new coordination polymer with the formula of [Co（2-mpac）2（py）·4H2O）]n （1, 2-mpac = 5-methyl-2-pyrazinecarboxylic acid, py = pyrazine） has been synthesized through hydrothermal synthesis and structurally ...A new coordination polymer with the formula of [Co（2-mpac）2（py）·4H2O）]n （1, 2-mpac = 5-methyl-2-pyrazinecarboxylic acid, py = pyrazine） has been synthesized through hydrothermal synthesis and structurally characterized by X-ray single-crystal diffraction method. 1 exhibits a 3D supramolecular network. Crystal data： monoclinic, space group C2/m, a = 15.907（9）, b = 7.104（3）, c = 9.793（5）, β = 95.232（8）o, V = 1102.0（10）3, Z = 2, S = 0.995, the final R = 0.0509, wR = 0.1626 for 1002 observed reflections with （I 〉 2σ（I）） and R = 0.0530, wR = 0.1667 for all data. In addition, elemental analysis, IR and thermalgravimetric analysis are presented.展开更多
基金supported partly by 0utstanding Young Scientist Award(#30125038)Key Project(#30230340)by Natural Science Foundation of China+2 种基金Chinese Key Basic Science Program by Ministry of Science and Technology of China(#2001CB510009)Foundation of Chinese Academy of Science(#KSCX2-2-08)UICC Yamagiwa-Yoshida Memorial International Cancer Study Grant.
文摘Type 2 cytokines are usually predominant in tumor patients and associated with tumor progression.To explore whether reversing of type 2 predominance could be a promising strategy in tumor immunotherapy,PBMCs of 35 lung cancer patients and 19 healthy subjects were prepared and subjected to be examined for cytokine secretion and gene expression.Tetra-Methylpyrazine (TTMP),extracted from a traditional Chinese medicinal herb which has been used in clinic to reverse the Th2 status of cancer patients in China,was added to PBMC culture. Determined by RT-PCR,the positive percentages of mRNA expression of type 1 cytokines (8.6% for IFN-γ and 11.4% for IL-2) were lower than those of type 2 cytokines (71.4% for IL-4,60% for IL-6 and 80% for 1L-10) in patients' PBMCs.The potential of gene expressing (measured as relative intensity to the ratio of β-actin) in the patients for type 1 cytokines was also in a low level (0.111 for IFN-γ,0.119 for IL-2) in comparison with a relative high level for type 2 cytokines (0.319 for IL-4,0.303 for IL-6 and 0.377 for IL-10).Meanwhile,both positive percentage and relative intensity of gene expression were lower for a type 1 cytokine-related transcription factor T-bet (31.4% and 0.142,respectively) than those for type 2 cytokine-related GATA3 (85.7% and 0.378, respectively).The blood serum levels of IFN-γ and IL-2 in the patients were slightly lower but not significantly when compared with healthy control.In contrast,the levels IL-4 and IL-6 in patients were significantly higher than those in healthy subjects by ELISA analysis.TTMP could enhance supernatant concentration and gene expression levels of IFN-γ,IL-2 and T-bet,but reduced those of type 2 cytokines.These results demonstrate that the lung cancer patients had a predominant expression of type 2 cytokines and TTMP could reverse the type 2 dominant status,which might offer an alternative therapeutic regime for lung cancer patients.Cellular & Molecular Immunology.2004;1(1):63-70.
文摘The 2-methylpyrazine was synthesized by catalytic reaction of ethylene diamine and propylene glycol at 380 ℃. The alumina supported copper catalysts with promoter were prepared by impregnation method, characterized by ICP-AES, BET and TPR. The results demonstrated that the dehydrogenation was improved by addition of chromium promoter. The selectivity of 2-methylpyrazine reached 84.75%, while the conversions of reactants were also enhanced.
基金Natural Science Foundation of Shaanxi Province (2009JQ2015,2010JM2009)Special Research Fund of Education Department of Shaanxi Province (09JK798, 2010JK902)
文摘A new coordination polymer with the formula of [Co（2-mpac）2（py）·4H2O）]n （1, 2-mpac = 5-methyl-2-pyrazinecarboxylic acid, py = pyrazine） has been synthesized through hydrothermal synthesis and structurally characterized by X-ray single-crystal diffraction method. 1 exhibits a 3D supramolecular network. Crystal data： monoclinic, space group C2/m, a = 15.907（9）, b = 7.104（3）, c = 9.793（5）, β = 95.232（8）o, V = 1102.0（10）3, Z = 2, S = 0.995, the final R = 0.0509, wR = 0.1626 for 1002 observed reflections with （I 〉 2σ（I）） and R = 0.0530, wR = 0.1667 for all data. In addition, elemental analysis, IR and thermalgravimetric analysis are presented.
