Background： Currently, various calculation methods for evaluating blood-loss in patients with total knee arthroplasty （TKA） are applied in clinical practice. However, different methods may yield different results. ...Background： Currently, various calculation methods for evaluating blood-loss in patients with total knee arthroplasty （TKA） are applied in clinical practice. However, different methods may yield different results. The purpose of this study was to determine the most reliable method for calculating blood-loss after primary TKA. Methods： We compared blood-loss in 245 patients who underwent primary unilateral TKA from February 2010 to August 2011. We calculated blood-loss using four methods： Gross equation, hemoglobin （Hb） balance, the Orthopedic Surgery Transfusion Hemoglobin European Overview （OSTHEO） formula, and Hb-dilution. We determined Pearson＇s correlation coefficients for the four methods. Results： There were large differences in the calculated blood-loss obtained by the four methods. In descending order of combined correlation coefficient based on calculated blood-loss, the methods were Hb-balance, OSTHEO formula, Hb-dilution, and Gross equation. Conclusions： The Hb-balance method may be the most reliable method of estimating blood-loss after TKA.展开更多
With the rapid development of DNA technologies, high throughput genomic data have become a powerful leverage to locate desirable genetic loci associated with traits of importance in various crop species. However, curr...With the rapid development of DNA technologies, high throughput genomic data have become a powerful leverage to locate desirable genetic loci associated with traits of importance in various crop species. However, current genetic association mapping analyses are focused on identifying individual QTLs. This study aimed to identify a set of QTLs or genetic markers, which can capture genetic variability for marker-assisted selection. Selecting a set with k loci that can maximize genetic variation out of high throughput genomic data is a challenging issue. In this study, we proposed an adaptive sequential replacement (ASR) method, which is considered a variant of the sequential replacement (SR) method. Through Monte Carlo simulation and comparing with four other selection methods: exhaustive, SR method, forward, and backward methods we found that the ASR method sustains consistent and repeatable results comparable to the exhaustive method with much reduced computational intensity.展开更多
Background Objective To summarize the nursing experience of 9 cases of double-hole thoracoscopic aortic valve replacement.Methods From February 2021 to February 2022,9 patientsdiagnosed with severe aortic valve diseas...Background Objective To summarize the nursing experience of 9 cases of double-hole thoracoscopic aortic valve replacement.Methods From February 2021 to February 2022,9 patientsdiagnosed with severe aortic valve disease were enrolled,including 6 males and 3 females,with an average age(50.89±5.3)years,all of whom used double-hole thoracoscopic aortic valve replacement.Implement targeted surgical nursing plans,strengthen condition monitoring,surgical nursing cooperation,and take effective nursing interventions to urgently respond to intraoperative complications.Results Among the 9 patients,7 patients underwent mechanical valve replacement and 2 patients underwent biovalve replacement,of which 2 patients underwent aortic root enlargement surgery at the same time,and none of the patients underwent intraoperative emergency transfer orthothoracotomy.The extracorporeal bypass time was(137.56±27.99)min,the aortic occlusion time was(95.33±17.96)min,and the surgical survival rate was 100%;All patients had well-placed aortic valves,and 2 had mild(<2 cm2)perivalvular leakage.The postoperative recovery time was short,the ventilation time was 6.13(4.43,19.99)h,of which 8 patients were extubated within24 hours,and the ICU length of stay was(2.68±1.3)days,and no deaths and complications occurred.Conclusion For patients with simple aortic valve disease,double-hole thoracoscopic aortic valve replacement is a safe and effective surgical treatment with good early clinical results.Implement a scientifically based nursing plan during surgery to effectively prevent complications,promote early recovery,and improve the quality of nursing.展开更多
Based on the crystal symmetry of [L-Met]^(B0) bovine insulin (LMBBI) and the fundamental theory of the molecular packing method, the scheme for the determination of the position and orientation of the molecules by onl...Based on the crystal symmetry of [L-Met]^(B0) bovine insulin (LMBBI) and the fundamental theory of the molecular packing method, the scheme for the determination of the position and orientation of the molecules by only using one-dimensional rotation and one-dimensional translation was chosen to be used,and therefore the calculation of the rotation function of the molecular replacement method and the refinement of the rotational and translational parameters by using the R-factor search method were simplified greatly. After the preliminary refinement by using the macromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemical-restrained least squares refinement technique assisted by the manual revision on the difference Fourier maps.The L-Met residues on the N-termlnus of the B-chain appeared clearly on the final electron density map.展开更多
文摘Background： Currently, various calculation methods for evaluating blood-loss in patients with total knee arthroplasty （TKA） are applied in clinical practice. However, different methods may yield different results. The purpose of this study was to determine the most reliable method for calculating blood-loss after primary TKA. Methods： We compared blood-loss in 245 patients who underwent primary unilateral TKA from February 2010 to August 2011. We calculated blood-loss using four methods： Gross equation, hemoglobin （Hb） balance, the Orthopedic Surgery Transfusion Hemoglobin European Overview （OSTHEO） formula, and Hb-dilution. We determined Pearson＇s correlation coefficients for the four methods. Results： There were large differences in the calculated blood-loss obtained by the four methods. In descending order of combined correlation coefficient based on calculated blood-loss, the methods were Hb-balance, OSTHEO formula, Hb-dilution, and Gross equation. Conclusions： The Hb-balance method may be the most reliable method of estimating blood-loss after TKA.
文摘With the rapid development of DNA technologies, high throughput genomic data have become a powerful leverage to locate desirable genetic loci associated with traits of importance in various crop species. However, current genetic association mapping analyses are focused on identifying individual QTLs. This study aimed to identify a set of QTLs or genetic markers, which can capture genetic variability for marker-assisted selection. Selecting a set with k loci that can maximize genetic variation out of high throughput genomic data is a challenging issue. In this study, we proposed an adaptive sequential replacement (ASR) method, which is considered a variant of the sequential replacement (SR) method. Through Monte Carlo simulation and comparing with four other selection methods: exhaustive, SR method, forward, and backward methods we found that the ASR method sustains consistent and repeatable results comparable to the exhaustive method with much reduced computational intensity.
基金supported by National Key Researchand Development Program of China(No.2022YFC2407406)2022 Stability Support for Innovative Capacity Building of Guangdong Provincial Scientific Research Institutions(No.KD022022015)Science and Technology Program of Guangzhou,China(No.202206010049).
文摘Background Objective To summarize the nursing experience of 9 cases of double-hole thoracoscopic aortic valve replacement.Methods From February 2021 to February 2022,9 patientsdiagnosed with severe aortic valve disease were enrolled,including 6 males and 3 females,with an average age(50.89±5.3)years,all of whom used double-hole thoracoscopic aortic valve replacement.Implement targeted surgical nursing plans,strengthen condition monitoring,surgical nursing cooperation,and take effective nursing interventions to urgently respond to intraoperative complications.Results Among the 9 patients,7 patients underwent mechanical valve replacement and 2 patients underwent biovalve replacement,of which 2 patients underwent aortic root enlargement surgery at the same time,and none of the patients underwent intraoperative emergency transfer orthothoracotomy.The extracorporeal bypass time was(137.56±27.99)min,the aortic occlusion time was(95.33±17.96)min,and the surgical survival rate was 100%;All patients had well-placed aortic valves,and 2 had mild(<2 cm2)perivalvular leakage.The postoperative recovery time was short,the ventilation time was 6.13(4.43,19.99)h,of which 8 patients were extubated within24 hours,and the ICU length of stay was(2.68±1.3)days,and no deaths and complications occurred.Conclusion For patients with simple aortic valve disease,double-hole thoracoscopic aortic valve replacement is a safe and effective surgical treatment with good early clinical results.Implement a scientifically based nursing plan during surgery to effectively prevent complications,promote early recovery,and improve the quality of nursing.
基金Project supported by the National Natural Science Foundation of China and the Bureau of Biology, Academia Sinica.
文摘Based on the crystal symmetry of [L-Met]^(B0) bovine insulin (LMBBI) and the fundamental theory of the molecular packing method, the scheme for the determination of the position and orientation of the molecules by only using one-dimensional rotation and one-dimensional translation was chosen to be used,and therefore the calculation of the rotation function of the molecular replacement method and the refinement of the rotational and translational parameters by using the R-factor search method were simplified greatly. After the preliminary refinement by using the macromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereochemical-restrained least squares refinement technique assisted by the manual revision on the difference Fourier maps.The L-Met residues on the N-termlnus of the B-chain appeared clearly on the final electron density map.
