利用碱溶、离心、破壁、酸沉对甲醇蛋白粗品进行纯化。研究了适合于甲醇酵母细胞破壁及胞内蛋白纯化的条件。结果表明:热碱与高压均质联合处理法较生物酶解处理法、热碱处理法及高压均质处理法对细胞破壁效率高;采用p H 10.0、60℃热处...利用碱溶、离心、破壁、酸沉对甲醇蛋白粗品进行纯化。研究了适合于甲醇酵母细胞破壁及胞内蛋白纯化的条件。结果表明:热碱与高压均质联合处理法较生物酶解处理法、热碱处理法及高压均质处理法对细胞破壁效率高;采用p H 10.0、60℃热处理2 h,100 MPa压力均质3次,细胞破壁率可达87.6%;在p H 4.5时,破壁后上清液蛋白沉淀量最大,所得纯化甲醇蛋白中粗蛋白质含量达到82.5%,纯化效果较为理想。展开更多
The low-heat-value cornstalk gas produced in the down-flow fixed bed gasifier was tentatively used for methanol synthesis. The cornstalk gas was purified and the technical procedures such as deoxygenation, desulfuriza...The low-heat-value cornstalk gas produced in the down-flow fixed bed gasifier was tentatively used for methanol synthesis. The cornstalk gas was purified and the technical procedures such as deoxygenation, desulfurization, catalytic cracking of tar, purification and hydrogenation were studied. The catalytic experiments of methanol synthesis with cornstalk syngas were carried out in a tubular-flow integral and isothermal reactor. The effect of reaction temperature, pressure, catalysttypes, catalyst particle size, syngas flow at entering end and composition of syngas was investigated. The optimum process conditions and yield of methanol from cornstalk syngas were obtained. The experimental results indicated that the proper catalyst of the synthetic reaction was C301 and the optimum catalyst size （φ） was 0.833 mm×0.351 mm. The optimum operating temperature and pressure were found to be 235℃ and 5 Mpa, respectively. The suitable syngas flow 0.9-1.10 mol/h at entering end was selected and the best composition of syngas were CO 10.49%, CO2 8.8%, N2 37.32%, CnHm 0.95% and H2 40.49%. The best methanol yield is 0.418 g/g cornstalk. The study provided the technical support for the industrial test of methanol production from biomass （cornstalk）gas.展开更多
文摘利用碱溶、离心、破壁、酸沉对甲醇蛋白粗品进行纯化。研究了适合于甲醇酵母细胞破壁及胞内蛋白纯化的条件。结果表明:热碱与高压均质联合处理法较生物酶解处理法、热碱处理法及高压均质处理法对细胞破壁效率高;采用p H 10.0、60℃热处理2 h,100 MPa压力均质3次,细胞破壁率可达87.6%;在p H 4.5时,破壁后上清液蛋白沉淀量最大,所得纯化甲醇蛋白中粗蛋白质含量达到82.5%,纯化效果较为理想。
基金Project supported by the Foundation of Science and Technology of Henan Province (No. 0324210047).
文摘The low-heat-value cornstalk gas produced in the down-flow fixed bed gasifier was tentatively used for methanol synthesis. The cornstalk gas was purified and the technical procedures such as deoxygenation, desulfurization, catalytic cracking of tar, purification and hydrogenation were studied. The catalytic experiments of methanol synthesis with cornstalk syngas were carried out in a tubular-flow integral and isothermal reactor. The effect of reaction temperature, pressure, catalysttypes, catalyst particle size, syngas flow at entering end and composition of syngas was investigated. The optimum process conditions and yield of methanol from cornstalk syngas were obtained. The experimental results indicated that the proper catalyst of the synthetic reaction was C301 and the optimum catalyst size （φ） was 0.833 mm×0.351 mm. The optimum operating temperature and pressure were found to be 235℃ and 5 Mpa, respectively. The suitable syngas flow 0.9-1.10 mol/h at entering end was selected and the best composition of syngas were CO 10.49%, CO2 8.8%, N2 37.32%, CnHm 0.95% and H2 40.49%. The best methanol yield is 0.418 g/g cornstalk. The study provided the technical support for the industrial test of methanol production from biomass （cornstalk）gas.
