China is one of countries with the highest mercury production in the world. The Guizhou Province in Southwestern China is currently one of the world's most important mercury production areas. In order to study the...China is one of countries with the highest mercury production in the world. The Guizhou Province in Southwestern China is currently one of the world's most important mercury production areas. In order to study the neurotoxicity of rice from Qingzhen Chemical Plant area and probe into the signal transduction molecular mechanism of injury in rat brain stimulation by mercury contaminated rice. The rats were exposed to mercury contaminated rice for 20 d. Both of the measurements of NO and NOS were processed according to the protocol of the kit. The effect of Hg contaminated rice on the expression of c-fos mRNA in rat brain and the expression of c-FOS protein in cortex, hippocampus were observed using reverse transcription polymerase chain reaction(RT-PCR) and immunocytochemical methods. The results showed the neural transmitter NO and NOS in brain were significantly change between exposure groups and control group; the mercury polluted rice induced significantly the expression of c-fos mRNA; the c-FOS positive cells in hippocampus and cortex of exposure groups were significant different from control group(p<0.01). It could be concluded that nitric oxide was involved in mercury contaminated rice induced immediate early gene c-fos expressions in the rat brain. Through food chain, local ecosystem and health of local people iave been deteriorated seriously by mercury. This serious situation will last a long period. In order to alleviate mercury pollution, more work needs to do.展开更多
为了探讨贵州万山汞矿汞污染生物健康效应,研究了万山8个月龄鸭子和万山粮食暴露20、30 d后大鼠脑、肝、血中MDA、SOD、GSH-px的变化以及大鼠大脑皮层中c-JUN蛋白表达.结果表明:在鸭血清、脑和肝组织中GSH-px和SOD活性与对照组相比均显...为了探讨贵州万山汞矿汞污染生物健康效应,研究了万山8个月龄鸭子和万山粮食暴露20、30 d后大鼠脑、肝、血中MDA、SOD、GSH-px的变化以及大鼠大脑皮层中c-JUN蛋白表达.结果表明:在鸭血清、脑和肝组织中GSH-px和SOD活性与对照组相比均显著升高(P<0.05),而MDA含量没有发生显著变化(P>0.05);万山组大鼠脑、肝、血中MDA、SOD、GSH-px的变化除暴露20 d MDA与对照组相比脑组织中含量显著升高(P<0.05)外,其他均无显著变化(P>0.05);大鼠大脑皮层c-JUN蛋白表达显著增加.从而可以推断,硒对汞引起的氧化损伤有很强的保护能力;汞对大鼠脑c-JUN蛋白表达的诱导不是或仅小部分由脂质过氧化所介导.展开更多
文摘China is one of countries with the highest mercury production in the world. The Guizhou Province in Southwestern China is currently one of the world's most important mercury production areas. In order to study the neurotoxicity of rice from Qingzhen Chemical Plant area and probe into the signal transduction molecular mechanism of injury in rat brain stimulation by mercury contaminated rice. The rats were exposed to mercury contaminated rice for 20 d. Both of the measurements of NO and NOS were processed according to the protocol of the kit. The effect of Hg contaminated rice on the expression of c-fos mRNA in rat brain and the expression of c-FOS protein in cortex, hippocampus were observed using reverse transcription polymerase chain reaction(RT-PCR) and immunocytochemical methods. The results showed the neural transmitter NO and NOS in brain were significantly change between exposure groups and control group; the mercury polluted rice induced significantly the expression of c-fos mRNA; the c-FOS positive cells in hippocampus and cortex of exposure groups were significant different from control group(p<0.01). It could be concluded that nitric oxide was involved in mercury contaminated rice induced immediate early gene c-fos expressions in the rat brain. Through food chain, local ecosystem and health of local people iave been deteriorated seriously by mercury. This serious situation will last a long period. In order to alleviate mercury pollution, more work needs to do.
文摘为了探讨贵州万山汞矿汞污染生物健康效应,研究了万山8个月龄鸭子和万山粮食暴露20、30 d后大鼠脑、肝、血中MDA、SOD、GSH-px的变化以及大鼠大脑皮层中c-JUN蛋白表达.结果表明:在鸭血清、脑和肝组织中GSH-px和SOD活性与对照组相比均显著升高(P<0.05),而MDA含量没有发生显著变化(P>0.05);万山组大鼠脑、肝、血中MDA、SOD、GSH-px的变化除暴露20 d MDA与对照组相比脑组织中含量显著升高(P<0.05)外,其他均无显著变化(P>0.05);大鼠大脑皮层c-JUN蛋白表达显著增加.从而可以推断,硒对汞引起的氧化损伤有很强的保护能力;汞对大鼠脑c-JUN蛋白表达的诱导不是或仅小部分由脂质过氧化所介导.
