The limitations of biomedecine to provide effective solutions to certain pandemics has led many people to turn to alternative therapies. These palliative solutions, particularly phytomedecines, are much sought after f...The limitations of biomedecine to provide effective solutions to certain pandemics has led many people to turn to alternative therapies. These palliative solutions, particularly phytomedecines, are much sought after for disease prevention and treatment. The Baka, a group of forest-dwelling people, hold knowledge on how wild plants can be effective in treating many illnesses. However, the greatest challenge for the identification of traditional medicines depends on the veracity of the information provided during ethnobotanic surveys by user populations. The present study describes forest plants used by the Baka and confirmed by them as being employed for traditional medicine. We carried out ethnobotanical surveys between 2019 and 2021 in 221 households within four districts of the Eastern and Southern Region of Cameroon. We used indices of significant use and performance applied to all mentioned species, alongside searches in the literature. The statistical tools used to distinguish the different groups/districts is Pearson’s X2 test. A total of 378 plant species were identified of 270 genera and 85 families. Ethnobotanical indices allowed to identify the most confirmed and efficient plants for several health problems. A pairwise comparison of these indices showed a significant correlation with a p-value < 2.2e-16 and a dissimilarity distance less than 0.5. Some plants selected are widely cited in other regions and/or countries for the same health problems. The presence of active molecules responsible for their biological activity was also proven, justifying their use in traditional medicine. This paper examines the potential to enhance the value of Cameroon’s pharmacopeia by integrating various ethnobotanical indices. It aims to discover new therapeutic molecules and develop improved traditional products from the diverse plant species documented. The findings indicate opportunities to advance both local and global healthcare solutions.展开更多
OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese medicine.METHODS...OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese medicine.METHODS:The normal group,six cold herb groups,six hot herb groups,six cool herb groups,PG prescription group(PGPG),and Dangshen(Radix Codonopsis)prescription group(CPPG)were prepared with corresponding concentrations of water extracts,these herb extracts were administered by gavage to SpragueDawley rats,and the 12 h urine at night on the 29th day of the SD rats in each group were collected,Liquid Chromatograph Mass Spectrometer system was used to analyze them,the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up,so as to the medicinal properties of PGPG,CPPG and PG were predicted.Based on the Progenesis QI,Human Metabolome Database,Kyoto Encyclopedia of Genes and Genomes,MetaboAnalyst 5.0 database,we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG prescription.RESULTS:We established a best model of cold-hot herbal discrimination in the positive ion mode,then the probability that PGPG was predicted cold property was 88.9%.Furthermore,a model of cold-cool herbal discrimination was established,then the probability of PGPG containing the cool property was 77.8%.In addition,typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline,(R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in rats.The regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine metabolism.CONCLUSION:The results showed PGPG had a cool medicinal property as same as CPPG,and the regulation trend of PGPG on markers was consistent with cool herbs.Therefore,the medicine properties PG and CP should be consistent,and the Traditional Chinese Medicine property of PG was predicted to be neutral.展开更多
文摘The limitations of biomedecine to provide effective solutions to certain pandemics has led many people to turn to alternative therapies. These palliative solutions, particularly phytomedecines, are much sought after for disease prevention and treatment. The Baka, a group of forest-dwelling people, hold knowledge on how wild plants can be effective in treating many illnesses. However, the greatest challenge for the identification of traditional medicines depends on the veracity of the information provided during ethnobotanic surveys by user populations. The present study describes forest plants used by the Baka and confirmed by them as being employed for traditional medicine. We carried out ethnobotanical surveys between 2019 and 2021 in 221 households within four districts of the Eastern and Southern Region of Cameroon. We used indices of significant use and performance applied to all mentioned species, alongside searches in the literature. The statistical tools used to distinguish the different groups/districts is Pearson’s X2 test. A total of 378 plant species were identified of 270 genera and 85 families. Ethnobotanical indices allowed to identify the most confirmed and efficient plants for several health problems. A pairwise comparison of these indices showed a significant correlation with a p-value < 2.2e-16 and a dissimilarity distance less than 0.5. Some plants selected are widely cited in other regions and/or countries for the same health problems. The presence of active molecules responsible for their biological activity was also proven, justifying their use in traditional medicine. This paper examines the potential to enhance the value of Cameroon’s pharmacopeia by integrating various ethnobotanical indices. It aims to discover new therapeutic molecules and develop improved traditional products from the diverse plant species documented. The findings indicate opportunities to advance both local and global healthcare solutions.
基金2018 National Key Research and Development Project:Research on Four Modes of Exploitation of New Sources of Rare and Endangered Chinese Medicine Resources-Research on Foreign Medicinal Materials(No.2018YFC1706100)Jiangxi Provincial Natural Science Foundation Key Project:Synthesis and Properties of Polysaccharide Moleculally-Imprinted Core-shell Microspheres based on Magnetic Micelle Technology(No.20192ACBL20028)。
文摘OBJECTIVE:To explore the four Qi of Pfaffia glomerata(PG)and endow this foreign folk herb with the properties of Chinese medicine,make it Chinese medicinal and localized,and could be used as a Chinese medicine.METHODS:The normal group,six cold herb groups,six hot herb groups,six cool herb groups,PG prescription group(PGPG),and Dangshen(Radix Codonopsis)prescription group(CPPG)were prepared with corresponding concentrations of water extracts,these herb extracts were administered by gavage to SpragueDawley rats,and the 12 h urine at night on the 29th day of the SD rats in each group were collected,Liquid Chromatograph Mass Spectrometer system was used to analyze them,the best discriminant models for the medicinal properties of cold-hot and cold-cool were set up,so as to the medicinal properties of PGPG,CPPG and PG were predicted.Based on the Progenesis QI,Human Metabolome Database,Kyoto Encyclopedia of Genes and Genomes,MetaboAnalyst 5.0 database,we enriched metabolic pathway and classification mechanism of medicinal properties of cold-cool Chinese herbs and the molecular mechanism of PG prescription.RESULTS:We established a best model of cold-hot herbal discrimination in the positive ion mode,then the probability that PGPG was predicted cold property was 88.9%.Furthermore,a model of cold-cool herbal discrimination was established,then the probability of PGPG containing the cool property was 77.8%.In addition,typical cold and cool herbs mainly affected nine biomarkers such as tyrosine-proline,(R)-3',7-Dihydroxy-2',4'-dimethoxyisoflavane in rats.The regulation trend of PGPG on markers was basically as same as the cool herbs and mainly involved in regulating the two pathways of cytochrome P450 and purine metabolism.CONCLUSION:The results showed PGPG had a cool medicinal property as same as CPPG,and the regulation trend of PGPG on markers was consistent with cool herbs.Therefore,the medicine properties PG and CP should be consistent,and the Traditional Chinese Medicine property of PG was predicted to be neutral.
