Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and ...Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), whi展开更多
In the 20th Century， modern pharmacology of traditional Chinese drugs （herbal pharmacology）has been developed by Chinese pharmacologists, clinicians and related scientists using modern technology.200～300 single ac...In the 20th Century， modern pharmacology of traditional Chinese drugs （herbal pharmacology）has been developed by Chinese pharmacologists, clinicians and related scientists using modern technology.200～300 single active ingredients have been isolated and a number of them being used in clinics．Modern pharmacological actions of nearly 400 recipes have been investigated and a number of jounals carrying papers on herbal pharmacology have been published． The development of herbal pharmacology could be roughly divided into two periods in the 20th Century ．In the first half of the Century actions of a number of herbs have been investigated and single compounds have been isolated.The dramatic event was the isolation and action study of ephedrine done by KK Chen． In the second half of the Century ，the study of herbal drugs has been carried out not only using modern technology but also under the guidance of the theory of TCM （traditional Chinese medicine）．The emphasis has been put on the sutdy of a group （or category） of herbs and recipes.Based on these studies， theories of mechanism of action of herbs is emerging． It is hoped in the next Century that the new and systematic modern herbal pharmacology will grow mature.And it is anticipated that there will be a great leap forward in the future decades．展开更多
基金Supported by the National Natural Science Foundation of China(No.30973694)
文摘Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), whi
文摘In the 20th Century， modern pharmacology of traditional Chinese drugs （herbal pharmacology）has been developed by Chinese pharmacologists, clinicians and related scientists using modern technology.200～300 single active ingredients have been isolated and a number of them being used in clinics．Modern pharmacological actions of nearly 400 recipes have been investigated and a number of jounals carrying papers on herbal pharmacology have been published． The development of herbal pharmacology could be roughly divided into two periods in the 20th Century ．In the first half of the Century actions of a number of herbs have been investigated and single compounds have been isolated.The dramatic event was the isolation and action study of ephedrine done by KK Chen． In the second half of the Century ，the study of herbal drugs has been carried out not only using modern technology but also under the guidance of the theory of TCM （traditional Chinese medicine）．The emphasis has been put on the sutdy of a group （or category） of herbs and recipes.Based on these studies， theories of mechanism of action of herbs is emerging． It is hoped in the next Century that the new and systematic modern herbal pharmacology will grow mature.And it is anticipated that there will be a great leap forward in the future decades．
