The experiment was conducted at Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI) and the genotypes were collected from Chapainawabganj, the most mango variability rich district in Bang...The experiment was conducted at Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI) and the genotypes were collected from Chapainawabganj, the most mango variability rich district in Bangladesh. The molecular characters of mango germplasm were assessed by using six simple sequence repeat (SSR) markers. Polymerase chain reaction (PCR) amplification of the DNA isolated from 60 mango germplasm with 6 SSR primers was performed. The sizes of the alleles detected ranged from 112 to 221 bp. SSRs exhibited moderate values of polymorphic information content (PIC) range of 0.9405 to 0.6501. Genetic distances (D) between varieties were computed from combined data of the 6 primers, ranging from 0.5000 to 1.0000. Moderate degree of genetic diversity was obtained where the highest level of gene diversity value was noted 0.9433 in loci MIGA179 and the lowest level of gene diversity value was computed 0.6683 in loci MIGA253 with a mean diversity of 0.8842. The dendrogram generated from the unweighed pair group arithmetic average (UPGMA) cluster analysis broadly placed 60 mango cultivars into ten major clusters. The cluster size varied from 1 to 12 and cluster-VI was the largest cluster comprising of 9 cultivars. The tendency of clustering among mango cultivars revealed that they have strong affinity towards further breeding programme.展开更多
The aim of this study was to characterize 103 mango accessions of the field germplasm collection of Embrapa semi-arid region, located in Juazeiro, Bahia, Brazil and to apply 50 morphological descriptors established by...The aim of this study was to characterize 103 mango accessions of the field germplasm collection of Embrapa semi-arid region, located in Juazeiro, Bahia, Brazil and to apply 50 morphological descriptors established by the Brazilian Ministry of Agriculture, livestock and supply to help in the development of new mango cultivars for the Northeastern region of Brazil. Four trees were selected for each accession and eight adult leaves, eight flowers and 16 fruits were collected from each tree. Morphological characteristics ranging from plant size to seed embryo were evaluated. Simple percentages were estimated for all the descriptors. Only the descriptors for leaf symmetry and fruit waxiness did not show variability among the accessions. Eight accessions did not show fruits with fibers, while nine other accessions presented flesh firmness, which is an important characteristic to improve breeding. The soluble solids content was high, above 14 ~Brix for 95% of the accessions with Tommy Atkins showing the lowest value, 12.5 ~Brix. A great diversity was found in the color of the epidermis ranging from green to red. The accessions Amrapali and Salitre presented a dark orange flesh color. The obtained data set, are the most comprehensive so far in Brazil, it allows choosing the best parents to develop new cultivars and will also contribute to the protection of mango cultivars in Brazil.展开更多
文摘The experiment was conducted at Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute (BARI) and the genotypes were collected from Chapainawabganj, the most mango variability rich district in Bangladesh. The molecular characters of mango germplasm were assessed by using six simple sequence repeat (SSR) markers. Polymerase chain reaction (PCR) amplification of the DNA isolated from 60 mango germplasm with 6 SSR primers was performed. The sizes of the alleles detected ranged from 112 to 221 bp. SSRs exhibited moderate values of polymorphic information content (PIC) range of 0.9405 to 0.6501. Genetic distances (D) between varieties were computed from combined data of the 6 primers, ranging from 0.5000 to 1.0000. Moderate degree of genetic diversity was obtained where the highest level of gene diversity value was noted 0.9433 in loci MIGA179 and the lowest level of gene diversity value was computed 0.6683 in loci MIGA253 with a mean diversity of 0.8842. The dendrogram generated from the unweighed pair group arithmetic average (UPGMA) cluster analysis broadly placed 60 mango cultivars into ten major clusters. The cluster size varied from 1 to 12 and cluster-VI was the largest cluster comprising of 9 cultivars. The tendency of clustering among mango cultivars revealed that they have strong affinity towards further breeding programme.
文摘The aim of this study was to characterize 103 mango accessions of the field germplasm collection of Embrapa semi-arid region, located in Juazeiro, Bahia, Brazil and to apply 50 morphological descriptors established by the Brazilian Ministry of Agriculture, livestock and supply to help in the development of new mango cultivars for the Northeastern region of Brazil. Four trees were selected for each accession and eight adult leaves, eight flowers and 16 fruits were collected from each tree. Morphological characteristics ranging from plant size to seed embryo were evaluated. Simple percentages were estimated for all the descriptors. Only the descriptors for leaf symmetry and fruit waxiness did not show variability among the accessions. Eight accessions did not show fruits with fibers, while nine other accessions presented flesh firmness, which is an important characteristic to improve breeding. The soluble solids content was high, above 14 ~Brix for 95% of the accessions with Tommy Atkins showing the lowest value, 12.5 ~Brix. A great diversity was found in the color of the epidermis ranging from green to red. The accessions Amrapali and Salitre presented a dark orange flesh color. The obtained data set, are the most comprehensive so far in Brazil, it allows choosing the best parents to develop new cultivars and will also contribute to the protection of mango cultivars in Brazil.
