Since 1995,three viral isolates named RGV9506,RGV9807 and RGV9808 associated with frog mortality were isolated from farm raised frogs (Rana grylio virus,RGV) in China.Both ultrastructural morphology and serological ch...Since 1995,three viral isolates named RGV9506,RGV9807 and RGV9808 associated with frog mortality were isolated from farm raised frogs (Rana grylio virus,RGV) in China.Both ultrastructural morphology and serological characterisitics showed that the RGV isolates belong to genus Ranavirus. The DNA sequence analysis of the 5’end of the major capsid protein(MCP) gene of RGV isolates by PCR amplification produced a 531bp fragment.DNA templates were prepared from cells infected with different RGV isolates and the specific primers designed were based on highly conserved region at the 5′ of the gene encoding Frog virus 3(FV3) MCP, which is the typical species of the genus Ranavirus.The PCR products indicated that the nucleotide sequence of MCP gene of the RGV9506,RGV9807 and RGV9808,showed 99.6%, 99.8% and 98.4% homology respectively with the corresponding region of the MCP gene of FV3.展开更多
文摘Since 1995,three viral isolates named RGV9506,RGV9807 and RGV9808 associated with frog mortality were isolated from farm raised frogs (Rana grylio virus,RGV) in China.Both ultrastructural morphology and serological characterisitics showed that the RGV isolates belong to genus Ranavirus. The DNA sequence analysis of the 5’end of the major capsid protein(MCP) gene of RGV isolates by PCR amplification produced a 531bp fragment.DNA templates were prepared from cells infected with different RGV isolates and the specific primers designed were based on highly conserved region at the 5′ of the gene encoding Frog virus 3(FV3) MCP, which is the typical species of the genus Ranavirus.The PCR products indicated that the nucleotide sequence of MCP gene of the RGV9506,RGV9807 and RGV9808,showed 99.6%, 99.8% and 98.4% homology respectively with the corresponding region of the MCP gene of FV3.
