The genetic transformation was achieved by pollen_mediated approach on maize ( Zea mays L.) inbred lines Tai 9101 and Zong 31. Plasmid DNA of pGLⅡ_RC_1 was mixed with fresh pollen of maize inbreds in sucrose ...The genetic transformation was achieved by pollen_mediated approach on maize ( Zea mays L.) inbred lines Tai 9101 and Zong 31. Plasmid DNA of pGLⅡ_RC_1 was mixed with fresh pollen of maize inbreds in sucrose solution. The pollens were treated by ultrasonication and collected, pollinated on silks of maize ears. Transformants were confirmed by dot blot hybridization, PCR amplification and PCR_Southern blot hybridization. The pollen_mediated transformation approach could circumvent the tedious tissue culture procedures like in particle bombardment and Agrobacterium infection, etc. This approach is simple, easy to operate, and could be widely used in practice.展开更多
目的采用高效毛细管电泳法测定玉米花粉多糖中单糖的组成。方法玉米花粉多糖水解后,经α-萘胺衍生化,高效毛细管电泳分析。电泳条件:缓冲液为75 mm o l/L硼砂溶液(pH 9.5);柱温22℃;电压16 kV;检测波长200 nm;气压进样,进样时间5 s。结...目的采用高效毛细管电泳法测定玉米花粉多糖中单糖的组成。方法玉米花粉多糖水解后,经α-萘胺衍生化,高效毛细管电泳分析。电泳条件:缓冲液为75 mm o l/L硼砂溶液(pH 9.5);柱温22℃;电压16 kV;检测波长200 nm;气压进样,进样时间5 s。结果玉米花粉多糖主要是由葡萄糖、阿拉伯糖和半乳糖3种单糖组成。结论高效毛细管电泳可用于测定多糖中单糖的组成。展开更多
文摘The genetic transformation was achieved by pollen_mediated approach on maize ( Zea mays L.) inbred lines Tai 9101 and Zong 31. Plasmid DNA of pGLⅡ_RC_1 was mixed with fresh pollen of maize inbreds in sucrose solution. The pollens were treated by ultrasonication and collected, pollinated on silks of maize ears. Transformants were confirmed by dot blot hybridization, PCR amplification and PCR_Southern blot hybridization. The pollen_mediated transformation approach could circumvent the tedious tissue culture procedures like in particle bombardment and Agrobacterium infection, etc. This approach is simple, easy to operate, and could be widely used in practice.
文摘目的采用高效毛细管电泳法测定玉米花粉多糖中单糖的组成。方法玉米花粉多糖水解后,经α-萘胺衍生化,高效毛细管电泳分析。电泳条件:缓冲液为75 mm o l/L硼砂溶液(pH 9.5);柱温22℃;电压16 kV;检测波长200 nm;气压进样,进样时间5 s。结果玉米花粉多糖主要是由葡萄糖、阿拉伯糖和半乳糖3种单糖组成。结论高效毛细管电泳可用于测定多糖中单糖的组成。
