利用基于串联质谱标签(Tandem mass tag,TMT)的蛋白质组学技术对长果桑(Morus macroura)的杂交品种台湾长果桑根部盐胁迫前后的差异蛋白进行鉴定和分析,筛选其响应盐胁迫的关键蛋白,初步解析其响应盐胁迫的蛋白质组学机制.结果发现,台...利用基于串联质谱标签(Tandem mass tag,TMT)的蛋白质组学技术对长果桑(Morus macroura)的杂交品种台湾长果桑根部盐胁迫前后的差异蛋白进行鉴定和分析,筛选其响应盐胁迫的关键蛋白,初步解析其响应盐胁迫的蛋白质组学机制.结果发现,台湾长果桑根部在100 mmol/L NaCl溶液处理4周后,与未处理的相比,差异蛋白表达量变化显著,共鉴定出160个差异蛋白.通过基因本体论(Gene ontology,GO)功能分类和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)代谢通路分析发现,这些差异蛋白功能涉及活性氧清除、胁迫防御、能量产生、碳水化合物代谢、转录翻译、生长发育、信号转导和物质转运、蛋白质折叠和合成、细胞骨架组成等.100 mmol/L NaCl胁迫处理下,二氢黄酮醇还原酶、原卟啉原氧化酶、果糖二磷酸醛缩酶、1,3-β-葡聚糖合酶等68个蛋白表达量显著上调,谷胱甘肽S-转移酶、蔗糖合成酶、甲基转移酶、过氧化物酶、丝氨酸/苏氨酸蛋白激酶、GTP(Guanosine-5-triphosphate)结合蛋白等92个蛋白表达量显著下调.根部差异蛋白对盐胁迫的响应,反映了台湾长果桑在蛋白功能表达和代谢途径上对盐胁迫的协调和自适应.展开更多
From the EtOH extract of the barks of Morus macroura Miq, a new benzofuran derivative, macrourin D l, together with four known benzofuran derivatives, macrourin B 2, 2-(3, 5-dihydroxyphenyl)-5, 6-dihydroxybenzofuran 3...From the EtOH extract of the barks of Morus macroura Miq, a new benzofuran derivative, macrourin D l, together with four known benzofuran derivatives, macrourin B 2, 2-(3, 5-dihydroxyphenyl)-5, 6-dihydroxybenzofuran 3, moracin M 4, and mulberroside C 5 were isolated, and their structures were determined on the basis of spectroscopic evidence and chemical correlation with known compounds.展开更多
A new flavanone named macrouroine C (1) was isolated from the bark of Morus macroura Miq.. The structure of 1 was elucidated mainly on the basis of spectroscopic evidence.
In order to incorporate the knowledge of two species of wild doves and compare the results of both, we assessed morphometrics (body weight, bill, tarsus, tail, wing, head and culmen length) and body condition index (u...In order to incorporate the knowledge of two species of wild doves and compare the results of both, we assessed morphometrics (body weight, bill, tarsus, tail, wing, head and culmen length) and body condition index (using weigh, wing, tail and tarsus length) of Eurasian Collared-Dove Streptopelia decaocto and Mourning Dove Zenaida macroura (Aves: Columbidae) based on 40 specimens of each species collected during fall-winter 2013 and spring-summer 2014 in the municipality of Durango, Durango, Mexico. We found that body condition index was higher during fallwinter for both species. We also found size dimorphism. Males of Eurasian Collared-Dove were larger than females in head length (p = 0.002) and tail length (p = 0.05) but smaller in culmen length. Female Mourning Doves had a higher body condition index compared to males (p = 0.02) during both seasons. As expected, Eurasian Collared-Dove was larger in all measurements than Mourning Dove (p > 0.05) but tail length (p = 0.12).展开更多
以含有飞燕草色素、矢车菊色素、天竺葵色素、芍药色素和锦葵色素的混合样本为标准,采用外标法定量法,探讨高效液相色谱法测定的最优条件,结果表明HPLC测定的最优条件为:Zorbax-C18色谱柱(250 mm×4.6 mm i.d.,5μm),流动相为7.5%...以含有飞燕草色素、矢车菊色素、天竺葵色素、芍药色素和锦葵色素的混合样本为标准,采用外标法定量法,探讨高效液相色谱法测定的最优条件,结果表明HPLC测定的最优条件为:Zorbax-C18色谱柱(250 mm×4.6 mm i.d.,5μm),流动相为7.5%甲酸乙腈-7.5%甲酸水溶液,流速1.0 mL·min^(-1),检测波长为525 nm。在此条件下测定桑树中台湾长桑、广东无籽大十2个品种的桑葚于青果期、转色期、成熟期的花青素成分及含量,结果表明台湾长桑在3个生长期的花青素含量明显低于广东无籽大十,矢车菊色素是桑葚的主要色素;HPLC方法简便、效果好。展开更多
文摘利用基于串联质谱标签(Tandem mass tag,TMT)的蛋白质组学技术对长果桑(Morus macroura)的杂交品种台湾长果桑根部盐胁迫前后的差异蛋白进行鉴定和分析,筛选其响应盐胁迫的关键蛋白,初步解析其响应盐胁迫的蛋白质组学机制.结果发现,台湾长果桑根部在100 mmol/L NaCl溶液处理4周后,与未处理的相比,差异蛋白表达量变化显著,共鉴定出160个差异蛋白.通过基因本体论(Gene ontology,GO)功能分类和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)代谢通路分析发现,这些差异蛋白功能涉及活性氧清除、胁迫防御、能量产生、碳水化合物代谢、转录翻译、生长发育、信号转导和物质转运、蛋白质折叠和合成、细胞骨架组成等.100 mmol/L NaCl胁迫处理下,二氢黄酮醇还原酶、原卟啉原氧化酶、果糖二磷酸醛缩酶、1,3-β-葡聚糖合酶等68个蛋白表达量显著上调,谷胱甘肽S-转移酶、蔗糖合成酶、甲基转移酶、过氧化物酶、丝氨酸/苏氨酸蛋白激酶、GTP(Guanosine-5-triphosphate)结合蛋白等92个蛋白表达量显著下调.根部差异蛋白对盐胁迫的响应,反映了台湾长果桑在蛋白功能表达和代谢途径上对盐胁迫的协调和自适应.
文摘From the EtOH extract of the barks of Morus macroura Miq, a new benzofuran derivative, macrourin D l, together with four known benzofuran derivatives, macrourin B 2, 2-(3, 5-dihydroxyphenyl)-5, 6-dihydroxybenzofuran 3, moracin M 4, and mulberroside C 5 were isolated, and their structures were determined on the basis of spectroscopic evidence and chemical correlation with known compounds.
文摘A new flavanone named macrouroine C (1) was isolated from the bark of Morus macroura Miq.. The structure of 1 was elucidated mainly on the basis of spectroscopic evidence.
文摘In order to incorporate the knowledge of two species of wild doves and compare the results of both, we assessed morphometrics (body weight, bill, tarsus, tail, wing, head and culmen length) and body condition index (using weigh, wing, tail and tarsus length) of Eurasian Collared-Dove Streptopelia decaocto and Mourning Dove Zenaida macroura (Aves: Columbidae) based on 40 specimens of each species collected during fall-winter 2013 and spring-summer 2014 in the municipality of Durango, Durango, Mexico. We found that body condition index was higher during fallwinter for both species. We also found size dimorphism. Males of Eurasian Collared-Dove were larger than females in head length (p = 0.002) and tail length (p = 0.05) but smaller in culmen length. Female Mourning Doves had a higher body condition index compared to males (p = 0.02) during both seasons. As expected, Eurasian Collared-Dove was larger in all measurements than Mourning Dove (p > 0.05) but tail length (p = 0.12).
