盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达...盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达的调控作用.在300 mM NaCl处理下,叶片中甘氨酸甜菜碱和Na+含量增加,编码CMO和NHX1的mRNA的表达水平升高、m^(6)A修饰水平降低、稳定性增强.这为m^(6)A修饰调控作物耐盐性提供了新的见解.展开更多
Eukaryotic gene expression is controlled by different levels of biological events, such as transcription factors regulating the timing and strength of transcripts production, alteration of transcription rate by RNA pr...Eukaryotic gene expression is controlled by different levels of biological events, such as transcription factors regulating the timing and strength of transcripts production, alteration of transcription rate by RNA processing, and mRNA stability during RNA processing and translation. RNAs, especially mRNAs, are relatively vulnerable molecules in living cells for ribonucleases （RNases）. The maintenance of quality and quantity of transcripts is a key issue for many biological processes. Extensive studies draw the conclusion that the stability of RNAs is dedicated-regulated, occurring co- and post-transcriptionally, and translation-coupled as well, either in the nucleus or cytoplasm. Recently, RNA stability in the nucleus has aroused much research interest, especially the stability of newly-made transcripts. In this article, we summarize recent progresses on mRNA stability in the nucleus, especially focusing on quality control of newly-made RNA by RNA polymerase Ⅱ in eukaryotes.展开更多
文摘盐分是降低作物产量的主要非生物胁迫因素.甜菜(Beta vulgaris)是耐盐性较强的经济作物,研究甜菜的耐盐机制具有重要意义.本研究以栽培甜菜O68品系为试验材料,利用m^(6)A-IP-qPCR探究了盐胁迫下m^(6)A修饰对甜菜耐盐基因CMO和NHX1表达的调控作用.在300 mM NaCl处理下,叶片中甘氨酸甜菜碱和Na+含量增加,编码CMO和NHX1的mRNA的表达水平升高、m^(6)A修饰水平降低、稳定性增强.这为m^(6)A修饰调控作物耐盐性提供了新的见解.
基金Project supported by the Talented Scientist Program from South China Agricultural University (No.4600-K14013)the National Natural Science Foundation of China (No.81301901)
文摘Eukaryotic gene expression is controlled by different levels of biological events, such as transcription factors regulating the timing and strength of transcripts production, alteration of transcription rate by RNA processing, and mRNA stability during RNA processing and translation. RNAs, especially mRNAs, are relatively vulnerable molecules in living cells for ribonucleases （RNases）. The maintenance of quality and quantity of transcripts is a key issue for many biological processes. Extensive studies draw the conclusion that the stability of RNAs is dedicated-regulated, occurring co- and post-transcriptionally, and translation-coupled as well, either in the nucleus or cytoplasm. Recently, RNA stability in the nucleus has aroused much research interest, especially the stability of newly-made transcripts. In this article, we summarize recent progresses on mRNA stability in the nucleus, especially focusing on quality control of newly-made RNA by RNA polymerase Ⅱ in eukaryotes.
