End-stage lung diseases are common and frequentlyoccurring diseases which are difficult for clinical treatment. In recent years, lung transplantation has become a widely accepted and effective therapeutic option for p...End-stage lung diseases are common and frequentlyoccurring diseases which are difficult for clinical treatment. In recent years, lung transplantation has become a widely accepted and effective therapeutic option for patients with the end-stage pulmonary diseases. Early pulmonary edema resulting from ischemia-reperfusion injury accounts for the major part of mortality and morbidity after lung transplantation. The water channel proteins in lung injury have been little studied, and their impact on the formation of pulmonary edema remains unclear. In this study, we established a rat lung ischemia-reperfusion model to study its impact on the expressions of water channel proteins in lung tissue and explore a new approach to lung transplantation in pulmonary edema pathogenesis.展开更多
目的研究在慢性阻塞性肺疾病(简称慢阻肺)大鼠肺组织中自噬相关蛋白的变化。方法单纯烟熏法构建慢阻肺大鼠动物模型,采用Real time PCR,Western blot技术检测检测大鼠肺组织中PI3K、AKT、p-AKT、m TOR、p-m TOR及自噬相关基因LC3Ⅱ/Ⅰ、...目的研究在慢性阻塞性肺疾病(简称慢阻肺)大鼠肺组织中自噬相关蛋白的变化。方法单纯烟熏法构建慢阻肺大鼠动物模型,采用Real time PCR,Western blot技术检测检测大鼠肺组织中PI3K、AKT、p-AKT、m TOR、p-m TOR及自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1、P62、Atg7、Atg12的mRNA及蛋白表达,探讨在慢阻肺大鼠肺组织中自噬水平及自噬相关蛋白的变化。用LC3B免疫组化比较COPD组与正常大鼠间自噬水平的变化。结果 Real time PCR分析结果显示,与正常组相比,慢阻肺组大鼠肺组织中自噬相关基因Beclin1、Atg5、Atg12的mRNA表达显著增高(P<0.05,其中Beclin1、Atg5两组比较P<0.01)。Atg7的mRNA表达在慢阻肺组与正常组之间差异无统计学意义(P>0.05)。Western blot分析结果显示,慢阻肺组大鼠肺组织中PI3K蛋白、p-AKT/AKT及p-m TOR/m TOR蛋白表达显著降低(P<0.05)。自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1蛋白表达增高(P<0.05,其中LC3Ⅱ/Ⅰ、Atg5两组比较P<0.01)。P62蛋白表达显著下降(P<0.01)。LC3B免疫组化显示,慢阻肺组LC3B表达高于正常组。结论烟熏12周的慢阻肺大鼠与正常组相比,自噬通路上游蛋白PI3K、p-AKT/AKT、p-m TOR/m TOR的表达降低,自噬蛋白表达增加,自噬水平增加。展开更多
文摘End-stage lung diseases are common and frequentlyoccurring diseases which are difficult for clinical treatment. In recent years, lung transplantation has become a widely accepted and effective therapeutic option for patients with the end-stage pulmonary diseases. Early pulmonary edema resulting from ischemia-reperfusion injury accounts for the major part of mortality and morbidity after lung transplantation. The water channel proteins in lung injury have been little studied, and their impact on the formation of pulmonary edema remains unclear. In this study, we established a rat lung ischemia-reperfusion model to study its impact on the expressions of water channel proteins in lung tissue and explore a new approach to lung transplantation in pulmonary edema pathogenesis.
文摘目的研究在慢性阻塞性肺疾病(简称慢阻肺)大鼠肺组织中自噬相关蛋白的变化。方法单纯烟熏法构建慢阻肺大鼠动物模型,采用Real time PCR,Western blot技术检测检测大鼠肺组织中PI3K、AKT、p-AKT、m TOR、p-m TOR及自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1、P62、Atg7、Atg12的mRNA及蛋白表达,探讨在慢阻肺大鼠肺组织中自噬水平及自噬相关蛋白的变化。用LC3B免疫组化比较COPD组与正常大鼠间自噬水平的变化。结果 Real time PCR分析结果显示,与正常组相比,慢阻肺组大鼠肺组织中自噬相关基因Beclin1、Atg5、Atg12的mRNA表达显著增高(P<0.05,其中Beclin1、Atg5两组比较P<0.01)。Atg7的mRNA表达在慢阻肺组与正常组之间差异无统计学意义(P>0.05)。Western blot分析结果显示,慢阻肺组大鼠肺组织中PI3K蛋白、p-AKT/AKT及p-m TOR/m TOR蛋白表达显著降低(P<0.05)。自噬相关基因LC3Ⅱ/Ⅰ、Atg5、Beclin1蛋白表达增高(P<0.05,其中LC3Ⅱ/Ⅰ、Atg5两组比较P<0.01)。P62蛋白表达显著下降(P<0.01)。LC3B免疫组化显示,慢阻肺组LC3B表达高于正常组。结论烟熏12周的慢阻肺大鼠与正常组相比,自噬通路上游蛋白PI3K、p-AKT/AKT、p-m TOR/m TOR的表达降低,自噬蛋白表达增加,自噬水平增加。
