Cysteine(Cys)plays a pivotal role in many physiological and pathological processes,including detoxification and protein synthesis.The abnormal levels of Cys are linked to many diseases.In this study,a novel red-emitti...Cysteine(Cys)plays a pivotal role in many physiological and pathological processes,including detoxification and protein synthesis.The abnormal levels of Cys are linked to many diseases.In this study,a novel red-emitting off-on fluorescent probe Cys-TCF was masterly constructed for discriminative detection of Cys.After a series of experimental assessment,Cys-TCF displayed higher selectivity and sensitivity for Cys over other biothilols with a low detection limit(0.04μmol/L).More notably,the probe was also successfully applied to image Cys in live cells and live zebrafishes with low cytotoxicity.展开更多
A new solid-contact Ag~＋-selective electrode was prepared with 9,10,12,13,24,25-hexahydro-5H,15H,23H-dibenzo [b,q][1,7,10,13,19,4,16]-entathiadiazacyclodocosine-6,16（7H,17H）-dione as ionophore,and cc,a-dihexylsexit...A new solid-contact Ag~＋-selective electrode was prepared with 9,10,12,13,24,25-hexahydro-5H,15H,23H-dibenzo [b,q][1,7,10,13,19,4,16]-entathiadiazacyclodocosine-6,16（7H,17H）-dione as ionophore,and cc,a-dihexylsexithiophene（DH- 6T） ion-to-electron transducer.The sensor exhibited a working concentration range of 10~8 to 10^（-3） mol/L,with a near-Nernstian slope of 55.1±0.2 mV/dec and detection limit of 1.7×10^（-9） mol/L.The fabricated electrodes demonstrated excellent selectivity over the most common monovalent and divalent cations.展开更多
基金financial supports from the National Natural Science Foundation of China (Nos.21708017,21572093,21778028)Lanzhou University (the Fundamental Research Funds for the Central Universities,No.lzujbky-2018-64)Natural Science Foundation of Gansu Province (No.17JR5RA193)
文摘Cysteine(Cys)plays a pivotal role in many physiological and pathological processes,including detoxification and protein synthesis.The abnormal levels of Cys are linked to many diseases.In this study,a novel red-emitting off-on fluorescent probe Cys-TCF was masterly constructed for discriminative detection of Cys.After a series of experimental assessment,Cys-TCF displayed higher selectivity and sensitivity for Cys over other biothilols with a low detection limit(0.04μmol/L).More notably,the probe was also successfully applied to image Cys in live cells and live zebrafishes with low cytotoxicity.
基金financially supported by the National Natural Science Foundation of China(No.21107134)Chinese Academy of Sciences(No.KZCX2-YW-JS208)+1 种基金the Natural Science Foundation of Shandong Province (JQ200814)the Taishan Scholar Program of Shandong Province
文摘A new solid-contact Ag~＋-selective electrode was prepared with 9,10,12,13,24,25-hexahydro-5H,15H,23H-dibenzo [b,q][1,7,10,13,19,4,16]-entathiadiazacyclodocosine-6,16（7H,17H）-dione as ionophore,and cc,a-dihexylsexithiophene（DH- 6T） ion-to-electron transducer.The sensor exhibited a working concentration range of 10~8 to 10^（-3） mol/L,with a near-Nernstian slope of 55.1±0.2 mV/dec and detection limit of 1.7×10^（-9） mol/L.The fabricated electrodes demonstrated excellent selectivity over the most common monovalent and divalent cations.
