黑土地在我国粮食安全中发挥着重要作用。中国科学院海伦农业生态实验站(简称“海伦站”)位于松嫩平原腹地,是中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)中唯一从事黑土农田生态系统长期定位监测、研究与示范的国...黑土地在我国粮食安全中发挥着重要作用。中国科学院海伦农业生态实验站(简称“海伦站”)位于松嫩平原腹地,是中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)中唯一从事黑土农田生态系统长期定位监测、研究与示范的国家级野外台站。按照CERN的统一规范,海伦站开展了土壤长期监测工作。本数据集收集、整理了海伦站2004–2015年3种不同施肥措施下表层(0–20 cm)土壤养分数据,包括土壤有机质、全氮、全磷、全钾、碱解氮、有效磷、速效钾、缓效钾、pH值、交换性阳离子(钙、镁、钾和钠)和阳离子交换量等14项指标,并附有完整的监测样地背景信息、样地管理记录、采样和分析方法记录以及质控信息。本数据集中10种土壤养分指标测定的相对误差平均为2.37%,重复测定的相对偏差为2.08%。本数据集可为黑土资源可持续利用、东北地区农田土壤肥力演变以及区域农业生态研究等工作提供数据基础。展开更多
Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental ...Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertil展开更多
文摘黑土地在我国粮食安全中发挥着重要作用。中国科学院海伦农业生态实验站(简称“海伦站”)位于松嫩平原腹地,是中国生态系统研究网络(Chinese Ecosystem Research Network,CERN)中唯一从事黑土农田生态系统长期定位监测、研究与示范的国家级野外台站。按照CERN的统一规范,海伦站开展了土壤长期监测工作。本数据集收集、整理了海伦站2004–2015年3种不同施肥措施下表层(0–20 cm)土壤养分数据,包括土壤有机质、全氮、全磷、全钾、碱解氮、有效磷、速效钾、缓效钾、pH值、交换性阳离子(钙、镁、钾和钠)和阳离子交换量等14项指标,并附有完整的监测样地背景信息、样地管理记录、采样和分析方法记录以及质控信息。本数据集中10种土壤养分指标测定的相对误差平均为2.37%,重复测定的相对偏差为2.08%。本数据集可为黑土资源可持续利用、东北地区农田土壤肥力演变以及区域农业生态研究等工作提供数据基础。
基金funded by the National Natural Science Foundation of China(NSFC31301843)the National Nonprofit Institute Research Grant of Chinese Academy of Agricultural Sciences(IARRP-202-5)
文摘Exploration of soil environmental characteristics governing soil microbial community structure and activity may improve our understanding of biogeochemical processes and soil quality. The impact of soil environmental characteristics especially organic carbon availability after 15-yr different organic and inorganic fertilizer inputs on soil bacterial community structure and functional metabolic diversity of soil microbial communities were evaluated in a 15-yr fertilizer experiment in Changping County, Beijing, China. The experiment was a wheat-maize rotation system which was established in 1991 including four different fertilizer treatments. These treatments included： a non-amended control（CK）, a commonly used application rate of inorganic fertilizer treatment（NPK）; a commonly used application rate of inorganic fertilizer with swine manure incorporated treatment（NPKM）, and a commonly used application rate of inorganic fertilizer with maize straw incorporated treatment（NPKS）. Denaturing gradient gel electrophoresis（DGGE） of the 16 S r RNA gene was used to determine the bacterial community structure and single carbon source utilization profiles were determined to characterize the microbial community functional metabolic diversity of different fertilizer treatments using Biolog Eco plates. The results indicated that long-term fertilized treatments significantly increased soil bacterial community structure compared to CK. The use of inorganic fertilizer with organic amendments incorporated for long term（NPKM, NPKS） significantly promoted soil bacterial structure than the application of inorganic fertilizer only（NPK）, and NPKM treatment was the most important driver for increases in the soil microbial community richness（S） and structural diversity（H）. Overall utilization of carbon sources by soil microbial communities（average well color development, AWCD） and microbial substrate utilization diversity and evenness indices（H＇ and E） indicated that long-term inorganic fertil
