Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot synd...Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot syndrome has occurred. In order to solve this dilemma, we have designed a novel co-delivery strategy to construct a co-loaded liposome of berberine(BER) and doxorubicin(DOX), which was called Lipo Be Do. The optimal synergistic ratio of the two drugs was screened by cell cytotoxicity experiments in vitro, and the optimal attenuation ratio was further determined by in vivo cardiac H&E staining pathological sections. The optimal combination treatment caused a robust increase in apoptotic cells of 4T1, as compared to drug alone treatment. The prepared co-loaded liposome, Lipo Be Do, had high encapsulation efficiency and good stability. The nanoliposome carrier controlled the biological fate of the drugs and maintained a pre-defined optimal ratio in vivo. The Lipo Be Do significantly inhibited tumor growth in 4T1 murine mammary carcinoma model compared with Doxil(P < 0.05), and completely overcame the myocardial rupture toxicity caused by Doxil in mice. Our co-loaded liposome delivery platform technology provided a new direction for the clinical treatment of triple-negative breast cancer and the safe application of DOX.展开更多
A model on the earthquake effects combination in wind resistant design of high-rise flexible structures is proposed in accordance with the probability method. Based on the Turkstra criteria, the stochastic characters ...A model on the earthquake effects combination in wind resistant design of high-rise flexible structures is proposed in accordance with the probability method. Based on the Turkstra criteria, the stochastic characters of wind velocity, earthquake ground acceleration and excitations occurrence probability are taken into account and then the combination of the earthquake effects in structure wind resistant design is analyzed with the convolution approach. The results indicate that as for the tall flexible buildings whose lateral force is governed by wind loading, the maximum lateral loads verification with respect to the wind resistant design combined with earthquake effects may be more unfavorable compared with that in terms of the earthquake resistant design involving wind effects.展开更多
基金the Career Development Program for Yong and Middle-aged Teachers in Shenyang Pharmaceutical University。
文摘Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot syndrome has occurred. In order to solve this dilemma, we have designed a novel co-delivery strategy to construct a co-loaded liposome of berberine(BER) and doxorubicin(DOX), which was called Lipo Be Do. The optimal synergistic ratio of the two drugs was screened by cell cytotoxicity experiments in vitro, and the optimal attenuation ratio was further determined by in vivo cardiac H&E staining pathological sections. The optimal combination treatment caused a robust increase in apoptotic cells of 4T1, as compared to drug alone treatment. The prepared co-loaded liposome, Lipo Be Do, had high encapsulation efficiency and good stability. The nanoliposome carrier controlled the biological fate of the drugs and maintained a pre-defined optimal ratio in vivo. The Lipo Be Do significantly inhibited tumor growth in 4T1 murine mammary carcinoma model compared with Doxil(P < 0.05), and completely overcame the myocardial rupture toxicity caused by Doxil in mice. Our co-loaded liposome delivery platform technology provided a new direction for the clinical treatment of triple-negative breast cancer and the safe application of DOX.
基金Project supported by the National Natural Science Foundation of China (No.50321803)
文摘A model on the earthquake effects combination in wind resistant design of high-rise flexible structures is proposed in accordance with the probability method. Based on the Turkstra criteria, the stochastic characters of wind velocity, earthquake ground acceleration and excitations occurrence probability are taken into account and then the combination of the earthquake effects in structure wind resistant design is analyzed with the convolution approach. The results indicate that as for the tall flexible buildings whose lateral force is governed by wind loading, the maximum lateral loads verification with respect to the wind resistant design combined with earthquake effects may be more unfavorable compared with that in terms of the earthquake resistant design involving wind effects.
