For investigating the accelerated blood clearance(ABC) phenomenon of polyglycerin modified nanoemulsions upon cross administration with polyethylene glycol(PEG) covered nanoemulsion, we used the 1,2-distea-royl-sn-gly...For investigating the accelerated blood clearance(ABC) phenomenon of polyglycerin modified nanoemulsions upon cross administration with polyethylene glycol(PEG) covered nanoemulsion, we used the 1,2-distea-royl-sn-glycero-3-phosphoethanolamine-npolyglycerine-610 and the 1,2-distearoyl-n-glycero-3-phosphoethanolamine-n-[methoxy(polyethylene glycol)-2000] as modify materials, the dialkylcarbocyanines as fluorescence indicator. Exhausted macrophages rat model was established and new material containing polycarboxyl structure was synthesized. The microplate reader and the in vivo optical imaging system were applied to measure the concentration of nanoemulsions in tissues.The results show that the first dose of polyglycerin modified nanoemulsion can induce the ABC phenomenon of the second dose of PEGylated nanoemulsion. With the increase in the amount of the surface polyglycerin, the extent of the ABC phenomenon decreases. Liver accumulation has positive relationship with the ABC phenomenon. Furthermore, kupffer cells in liver can get more immune information from polyhydroxy structure than polycarboxyl group in the modify compound. The results of our work imply that the polycarboxyl structure has advantages to eliminate the ABC phenomenon.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos.81072602,81373334)
文摘For investigating the accelerated blood clearance(ABC) phenomenon of polyglycerin modified nanoemulsions upon cross administration with polyethylene glycol(PEG) covered nanoemulsion, we used the 1,2-distea-royl-sn-glycero-3-phosphoethanolamine-npolyglycerine-610 and the 1,2-distearoyl-n-glycero-3-phosphoethanolamine-n-[methoxy(polyethylene glycol)-2000] as modify materials, the dialkylcarbocyanines as fluorescence indicator. Exhausted macrophages rat model was established and new material containing polycarboxyl structure was synthesized. The microplate reader and the in vivo optical imaging system were applied to measure the concentration of nanoemulsions in tissues.The results show that the first dose of polyglycerin modified nanoemulsion can induce the ABC phenomenon of the second dose of PEGylated nanoemulsion. With the increase in the amount of the surface polyglycerin, the extent of the ABC phenomenon decreases. Liver accumulation has positive relationship with the ABC phenomenon. Furthermore, kupffer cells in liver can get more immune information from polyhydroxy structure than polycarboxyl group in the modify compound. The results of our work imply that the polycarboxyl structure has advantages to eliminate the ABC phenomenon.
文摘研究鱼油对高脂模型小鼠血脂的改善作用。将6—7周龄雄性C57BL/6J小鼠分为三组,饲喂正常饮食、高脂饮食或高脂补充鱼油18周后,测定体重、食物摄入量、肝脏指数和空腹血糖,并用酶法检测血清中甘油三酯(Triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)浓度及游离脂肪酸(nonestesterified fatty acid,NEFA)含量。通过使用苏木精—伊红染色法及油红O染色法评价了肝组织形态学改变。结果表明,在高脂饮食小鼠中,体重增加,肝脏甘油三酯(TG)水平较正常小鼠高出约38%(P为0.0028);与高脂饮食小鼠相比,高脂补充鱼油的小鼠TG降低了6.80%(P为0.6738)、LDL-C降低了33.23%(P为0.0023),HDL-C水平显著性提升了51.25%,此外,高脂补充鱼油组小鼠表现出更大的代谢变化,脂肪堆积减少33%(P为0.1207)。这些结果表明鱼油补充对高脂饮食诱导脂代谢的有益影响,鱼油可能通过调节脂肪代谢来改善高脂饮食引起的肥胖,血脂异常的潜力,这一结果可以应用于预防肥胖的个性化营养。
