Obstructive azoospermia secondary to epididymal obstruction can be corrected by microsurgical reconstruction with vasoepididymostomy （VE）. Although alternative management such as epididymal or testicular sperm aspir...Obstructive azoospermia secondary to epididymal obstruction can be corrected by microsurgical reconstruction with vasoepididymostomy （VE）. Although alternative management such as epididymal or testicular sperm aspiration in conjunction with intracytoplasmic sperm injection is feasible, various studies have established the superior cost-effectiveness of VE as a treatment of choice. Microsurgical VE is considered one of the most technically challenging microsurgeries. Its success rate is highly dependent on the skills and experience of the surgeons. Various techniques have been described in the literature for VE. We have pioneered a technique known as longitudinal intussusception VE （LIVE） in which the epididymal tubule is opened longitudinally to obtain a larger opening to allow its tubular content to pass through the anastomosis. Our preliminary data demonstrated a patency rate of over 90%. This technique has been widely referenced in the recent literature including robotic-assisted microsurgery. The history of the development of different VE approaches, the oreoDerative evaluation along with the techniaues of various VE will be described in this article.展开更多
目的探讨 HBV DNA 对胰腺的致病作用.方法应用地高辛素标记 HBV DNA s 577bp 探针原位杂交(ISH)和免疫组织化学技术,检测12例血清 HBV 标志物阳性的肝硬变患者肝、胰组织石蜡包埋切片 HBV DNA 的存在.结果 ISH 采用 HBV DNA S 577bp 探...目的探讨 HBV DNA 对胰腺的致病作用.方法应用地高辛素标记 HBV DNA s 577bp 探针原位杂交(ISH)和免疫组织化学技术,检测12例血清 HBV 标志物阳性的肝硬变患者肝、胰组织石蜡包埋切片 HBV DNA 的存在.结果 ISH 采用 HBV DNA S 577bp 探针检测肝组织11例(11/12)阳性,糖耐量试验(GTT)异常7例 HBV DNA 均阳性,GTT 正常者阳性4例(4/5).胰腺组织阳性8例(8/12),GTT异常7例均阳性,GTT 正常者1例阳性(1/5),HBV DNA 阳性主要位于肝细胞、胰腺腺泡细胞和胰岛细胞的细胞核内.免疫组织化学法检测肝组织 HBsAg 均阳性,胰腺组织阳性10例(10/12),主要在胞质;而 HBcAg 在肝组织阳性5例(5/12),胰腺组织阳性5例(5/12)主要分布在细胞核,胞质分布量较少.结论 HBV 可直接侵害胰腺腺泡细胞和胰岛细胞,它可能是造成 HBV 感染后并发糖尿病的直接原因.展开更多
本文以系统功能语言学为理论根据,以言语功能和互动模式为分析框架,以美国CNN电视访谈节目Larry King Live作为分析语料,考察在电视媒介下会话参与者的交流互动和意义磋商过程,目的是探讨在电视访谈中主持人及嘉宾之间存在的语言互动模...本文以系统功能语言学为理论根据,以言语功能和互动模式为分析框架,以美国CNN电视访谈节目Larry King Live作为分析语料,考察在电视媒介下会话参与者的交流互动和意义磋商过程,目的是探讨在电视访谈中主持人及嘉宾之间存在的语言互动模式。本研究表明:在我们所分析的语料中,存在3种语言互动模式:合作型、挑衅型及回避型。展开更多
文摘Obstructive azoospermia secondary to epididymal obstruction can be corrected by microsurgical reconstruction with vasoepididymostomy （VE）. Although alternative management such as epididymal or testicular sperm aspiration in conjunction with intracytoplasmic sperm injection is feasible, various studies have established the superior cost-effectiveness of VE as a treatment of choice. Microsurgical VE is considered one of the most technically challenging microsurgeries. Its success rate is highly dependent on the skills and experience of the surgeons. Various techniques have been described in the literature for VE. We have pioneered a technique known as longitudinal intussusception VE （LIVE） in which the epididymal tubule is opened longitudinally to obtain a larger opening to allow its tubular content to pass through the anastomosis. Our preliminary data demonstrated a patency rate of over 90%. This technique has been widely referenced in the recent literature including robotic-assisted microsurgery. The history of the development of different VE approaches, the oreoDerative evaluation along with the techniaues of various VE will be described in this article.
文摘目的探讨 HBV DNA 对胰腺的致病作用.方法应用地高辛素标记 HBV DNA s 577bp 探针原位杂交(ISH)和免疫组织化学技术,检测12例血清 HBV 标志物阳性的肝硬变患者肝、胰组织石蜡包埋切片 HBV DNA 的存在.结果 ISH 采用 HBV DNA S 577bp 探针检测肝组织11例(11/12)阳性,糖耐量试验(GTT)异常7例 HBV DNA 均阳性,GTT 正常者阳性4例(4/5).胰腺组织阳性8例(8/12),GTT异常7例均阳性,GTT 正常者1例阳性(1/5),HBV DNA 阳性主要位于肝细胞、胰腺腺泡细胞和胰岛细胞的细胞核内.免疫组织化学法检测肝组织 HBsAg 均阳性,胰腺组织阳性10例(10/12),主要在胞质;而 HBcAg 在肝组织阳性5例(5/12),胰腺组织阳性5例(5/12)主要分布在细胞核,胞质分布量较少.结论 HBV 可直接侵害胰腺腺泡细胞和胰岛细胞,它可能是造成 HBV 感染后并发糖尿病的直接原因.
文摘本文以系统功能语言学为理论根据,以言语功能和互动模式为分析框架,以美国CNN电视访谈节目Larry King Live作为分析语料,考察在电视媒介下会话参与者的交流互动和意义磋商过程,目的是探讨在电视访谈中主持人及嘉宾之间存在的语言互动模式。本研究表明:在我们所分析的语料中,存在3种语言互动模式:合作型、挑衅型及回避型。
