BACKGROUND Linked color imaging(LCI) is a method of endoscopic imaging that emphasizes slight differences in red mucosal color.AIM To evaluate LCI in diagnostic endoscopy of early gastric cancer and to compare LCI and...BACKGROUND Linked color imaging(LCI) is a method of endoscopic imaging that emphasizes slight differences in red mucosal color.AIM To evaluate LCI in diagnostic endoscopy of early gastric cancer and to compare LCI and pathological findings.METHODS Endoscopic images were obtained for 39 patients(43 lesions) with early gastric cancer. Three endoscopists evaluated lesion recognition with white light imaging(WLI) and LCI. Color values in Commission Internationale de l'Eclairage(CIE)1976 L*a*b* color space were used to calculate the color difference(ΔE) between cancer lesions and non-cancer areas. After endoscopic submucosal dissection,blood vessel density in the surface layer of the gastric epithelium was evaluated pathologically. The identical region of interest was selected for analyses of endoscopic images(WLI and LCI) and pathological analyses.RESULTS LCI was superior for lesion recognition(P < 0.0001), and ΔE between cancer and non-cancer areas was significantly greater with LCI than WLI(29.4 vs 18.6, P <0.0001). Blood vessel density was significantly higher in cancer lesions(5.96% vs4.15%, P = 0.0004). An a* cut-off of ≥ 24 in CIE 1976 L*a*b* color space identified a cancer lesion using LCI with sensitivity of 76.7%, specificity of 93.0%, and accuracy of 84.9%.CONCLUSION LCI is more effective for recognition of early gastric cancer compared to WLI as a result of improved visualization of changes in redness. Surface blood vessel density was significantly higher in cancer lesions, and this result is consistent with LCI image analysis.展开更多
目的研究对Tb舌癌细胞的整合素连接激酶(ILK)进行特异性siRNA沉默后,下游信号分子Akt、GSK3β磷酸化状态及对移植瘤生长和自发性转移的变化。方法建立特异性si ILK表达载体和无同源性的对照载体,通过Lipofectamine 2000介导稳定转染人...目的研究对Tb舌癌细胞的整合素连接激酶(ILK)进行特异性siRNA沉默后,下游信号分子Akt、GSK3β磷酸化状态及对移植瘤生长和自发性转移的变化。方法建立特异性si ILK表达载体和无同源性的对照载体,通过Lipofectamine 2000介导稳定转染人舌鳞癌Tb细胞,然后将Tb、Tb vector和Tb silk 3组细胞分别注入裸鼠皮下构建移植瘤模型,5周后检测瘤大小和质量,对裸鼠肺及瘤组织行常规病理学检查,用免疫荧光技术检测癌细胞的p-Akt和p-GSK3β等的表达,并对瘤组织内血管生成情况进行观察。结果 Tb组、Tb vector组肺组织均发现自发性转移瘤,Tb si ILK组肺组织未发现自发性转移瘤;Tb si ILK组移植瘤细胞形态较其他二组体积减小,核分裂相较少,核质比例缩小;Tb si ILK组在体内和体外实验中的p-Akt和p-GSK3β表达较其他二组均明显下降;Tb si ILK组移植瘤质量(1.68±1.35)g较Tb组(3.58±1.04)g与Tb vector组(3.64±0.65)g分别降低了53.0%和53.8%(P<0.05);Tb si ILK组移植瘤血管[(5.6±2.2)/视野]生成较Tb组[(15.3±2.3)/视野]和Tb vector组[(14.6±1.4)/视野]也明显减少(P<0.05)。结论特异性ILK表达沉默抑制Tb舌癌移植瘤的血管生成和瘤细胞增殖,可能与其抑制下游信号传导分子Akt及GSK3β的磷酸化有关,提示ILK有望成为肿瘤治疗的靶基因。展开更多
文摘BACKGROUND Linked color imaging(LCI) is a method of endoscopic imaging that emphasizes slight differences in red mucosal color.AIM To evaluate LCI in diagnostic endoscopy of early gastric cancer and to compare LCI and pathological findings.METHODS Endoscopic images were obtained for 39 patients(43 lesions) with early gastric cancer. Three endoscopists evaluated lesion recognition with white light imaging(WLI) and LCI. Color values in Commission Internationale de l'Eclairage(CIE)1976 L*a*b* color space were used to calculate the color difference(ΔE) between cancer lesions and non-cancer areas. After endoscopic submucosal dissection,blood vessel density in the surface layer of the gastric epithelium was evaluated pathologically. The identical region of interest was selected for analyses of endoscopic images(WLI and LCI) and pathological analyses.RESULTS LCI was superior for lesion recognition(P < 0.0001), and ΔE between cancer and non-cancer areas was significantly greater with LCI than WLI(29.4 vs 18.6, P <0.0001). Blood vessel density was significantly higher in cancer lesions(5.96% vs4.15%, P = 0.0004). An a* cut-off of ≥ 24 in CIE 1976 L*a*b* color space identified a cancer lesion using LCI with sensitivity of 76.7%, specificity of 93.0%, and accuracy of 84.9%.CONCLUSION LCI is more effective for recognition of early gastric cancer compared to WLI as a result of improved visualization of changes in redness. Surface blood vessel density was significantly higher in cancer lesions, and this result is consistent with LCI image analysis.
文摘目的研究对Tb舌癌细胞的整合素连接激酶(ILK)进行特异性siRNA沉默后,下游信号分子Akt、GSK3β磷酸化状态及对移植瘤生长和自发性转移的变化。方法建立特异性si ILK表达载体和无同源性的对照载体,通过Lipofectamine 2000介导稳定转染人舌鳞癌Tb细胞,然后将Tb、Tb vector和Tb silk 3组细胞分别注入裸鼠皮下构建移植瘤模型,5周后检测瘤大小和质量,对裸鼠肺及瘤组织行常规病理学检查,用免疫荧光技术检测癌细胞的p-Akt和p-GSK3β等的表达,并对瘤组织内血管生成情况进行观察。结果 Tb组、Tb vector组肺组织均发现自发性转移瘤,Tb si ILK组肺组织未发现自发性转移瘤;Tb si ILK组移植瘤细胞形态较其他二组体积减小,核分裂相较少,核质比例缩小;Tb si ILK组在体内和体外实验中的p-Akt和p-GSK3β表达较其他二组均明显下降;Tb si ILK组移植瘤质量(1.68±1.35)g较Tb组(3.58±1.04)g与Tb vector组(3.64±0.65)g分别降低了53.0%和53.8%(P<0.05);Tb si ILK组移植瘤血管[(5.6±2.2)/视野]生成较Tb组[(15.3±2.3)/视野]和Tb vector组[(14.6±1.4)/视野]也明显减少(P<0.05)。结论特异性ILK表达沉默抑制Tb舌癌移植瘤的血管生成和瘤细胞增殖,可能与其抑制下游信号传导分子Akt及GSK3β的磷酸化有关,提示ILK有望成为肿瘤治疗的靶基因。
