To detect distribution and relative frequency of diffuse neuroendocrine system （DNES） cells in the gastrointestinal tract of channel catfish （lctalurus punctatus）, the intestinal tract of channel catfish was divid...To detect distribution and relative frequency of diffuse neuroendocrine system （DNES） cells in the gastrointestinal tract of channel catfish （lctalurus punctatus）, the intestinal tract of channel catfish was divided into seven portions from proximal to distal： the enlarged area after oesophagus, cardia, fundus, pylorus, and anterior, middle, and posterior intestine. Immunohistochemical method using the strept avidin-biotin-complex （SABC） was employed. All antisera between seven portions of the channel catfish were compared statistically using statistical package for the social science （SPSS）. Five types of DNES ceils were determined： neuropeptide Y-immunoreactive （NPY-IR） cells were demonstrated in both anterior and middle intestine; serotonin （5-HT） immunoreactive cells were detected throughout the whole gastrointestinal tract; vasoactive intestinal peptide （VIP） positive cells were at the highest frequency in pylorus; glucagon-immunoreactive （GLU-IR） cells were moderate in number in the fundus and anterior, middle intestine, and no immunoreactivity was determined in the other portions; somatostatin （SOM） positive cells were more abundant in the anterior and middle intestine. The regional distribution and relative frequency of immunoreactive cells in the channel catfish, Ictalurus punctatus, are essentially similar to those of other fish. However, some characteristics are observed in this species, which further proved that the diversity of the physiological function of DNES cells was based on their morphology.展开更多
Three ponds with the areas of 800, 1 000 and 1 000 m2 were settled in Yambajan aquaculture base for rearing test of Ictalurus punctatus in plateau zone. The results showed that as 30 000 fries were released, 19 830 fi...Three ponds with the areas of 800, 1 000 and 1 000 m2 were settled in Yambajan aquaculture base for rearing test of Ictalurus punctatus in plateau zone. The results showed that as 30 000 fries were released, 19 830 fingerlings of 1. punctatus were obtained after 270 d rearing, with the average body length of 21.2 cm, the average body weight of 216.5 g, the survival rate of 66.1%, and the fingerling harvest of 4 293.2 kg. Thus, it is effective and feasible to rear I. punctatus with residual heat resources of power plants in cold plateau zone.展开更多
基金supported by the Special Founda-tion for Young Leaders Fellowship in the Sichuan Agri-cultural University, China (45078925-5).
文摘To detect distribution and relative frequency of diffuse neuroendocrine system （DNES） cells in the gastrointestinal tract of channel catfish （lctalurus punctatus）, the intestinal tract of channel catfish was divided into seven portions from proximal to distal： the enlarged area after oesophagus, cardia, fundus, pylorus, and anterior, middle, and posterior intestine. Immunohistochemical method using the strept avidin-biotin-complex （SABC） was employed. All antisera between seven portions of the channel catfish were compared statistically using statistical package for the social science （SPSS）. Five types of DNES ceils were determined： neuropeptide Y-immunoreactive （NPY-IR） cells were demonstrated in both anterior and middle intestine; serotonin （5-HT） immunoreactive cells were detected throughout the whole gastrointestinal tract; vasoactive intestinal peptide （VIP） positive cells were at the highest frequency in pylorus; glucagon-immunoreactive （GLU-IR） cells were moderate in number in the fundus and anterior, middle intestine, and no immunoreactivity was determined in the other portions; somatostatin （SOM） positive cells were more abundant in the anterior and middle intestine. The regional distribution and relative frequency of immunoreactive cells in the channel catfish, Ictalurus punctatus, are essentially similar to those of other fish. However, some characteristics are observed in this species, which further proved that the diversity of the physiological function of DNES cells was based on their morphology.
文摘Three ponds with the areas of 800, 1 000 and 1 000 m2 were settled in Yambajan aquaculture base for rearing test of Ictalurus punctatus in plateau zone. The results showed that as 30 000 fries were released, 19 830 fingerlings of 1. punctatus were obtained after 270 d rearing, with the average body length of 21.2 cm, the average body weight of 216.5 g, the survival rate of 66.1%, and the fingerling harvest of 4 293.2 kg. Thus, it is effective and feasible to rear I. punctatus with residual heat resources of power plants in cold plateau zone.
