The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vas- oactive intestinal peptide receptor-1 （VIPR-1） was selected as the candidate gene for identifying traits of eg...The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vas- oactive intestinal peptide receptor-1 （VIPR-1） was selected as the candidate gene for identifying traits of egg produc- tion. A single nucleotide polymorphism （SNP） detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Two mutations （G373T, A313G） were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were sig- nificantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium （LD） analysis in- dicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the hlh2 （GGGT） diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.展开更多
This study was carried out to evaluate the effect of vitE(Vitamin E)supplementation in combination with vitC(Vitamin C)on reproductive performance and hatching rate of Japanese quail at the age of 59-114 days.A total ...This study was carried out to evaluate the effect of vitE(Vitamin E)supplementation in combination with vitC(Vitamin C)on reproductive performance and hatching rate of Japanese quail at the age of 59-114 days.A total of 132 laying quails were arranged in a completely randomized design with 3 treatments and 4 replicates,each replicate being 9 female quails and 2 male quails at 59 days of age.The experimental treatments were as follows:control fed the basal diet(KPCS),E125C75 including KPCS supplemented with 125 mg vitE/kg feed combined with 75 mg vitC/kg feed and E125C125 including KPCS supplemented 125 mg vitE/kg feed combined with 125 mg vitC/kg feed.Research results showed that the highest WG(Weight Gain)was in E125C125(30.32 g)and lowest in E125C75(17.37 g).There were no statistically significant differences between treatments in terms of laying rate,feed conversion ratio,egg mass and egg weight.However,hatching rate and bodyweight of quails at 1 day of age were significantly different between treatments,the highest was E125C125(74.37%and 7.43 g),followed by E125C75(70.02%and 7.03 g)and the lowest was control(65.89%and 6.82 g).It can be concluded that E125C125 not only improved laying rate but also increased hatching rate and bodyweight of quail chick.展开更多
基金supported by the Open Project of Hubei Key Laboratory of Animal Embryo and Molecular Breeding(No.2015ZD146),China
文摘The laying quail is a worldwide breed which exhibits high economic value. In our current study, the vas- oactive intestinal peptide receptor-1 （VIPR-1） was selected as the candidate gene for identifying traits of egg produc- tion. A single nucleotide polymorphism （SNP） detection was performed in 443 individual quails, including 196 quails from the H line, 202 quails from the L line, and 45 wild quails. The SNPs were genotyped using a polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Two mutations （G373T, A313G） were detected in all the tested quail populations. The associated analysis showed that the SNP genotypes of the VIPR-1 gene were sig- nificantly linked with the egg weight of G373T and A313G in 398 quails. The quails with the genotype GG always exhibited the largest egg weight for the two mutations in the H and L lines. Linkage disequilibrium （LD） analysis in- dicated that G373T and A313G loci showed the weakest LD. Seven main diplotypes from the four main reconstructed haplotypes were observed, indicating a significant association of diplotypes with egg weight. Quails with the hlh2 （GGGT） diplotype always exhibited the smallest egg weight and largest egg number at 20 weeks of age. The overall results suggest that the alterations in quails may be linked with potential major loci or genes affecting reproductive traits.
基金funded in part by the Can Tho University Improvement Project VN14-P6 supported by a Japanese ODA loan.
文摘This study was carried out to evaluate the effect of vitE(Vitamin E)supplementation in combination with vitC(Vitamin C)on reproductive performance and hatching rate of Japanese quail at the age of 59-114 days.A total of 132 laying quails were arranged in a completely randomized design with 3 treatments and 4 replicates,each replicate being 9 female quails and 2 male quails at 59 days of age.The experimental treatments were as follows:control fed the basal diet(KPCS),E125C75 including KPCS supplemented with 125 mg vitE/kg feed combined with 75 mg vitC/kg feed and E125C125 including KPCS supplemented 125 mg vitE/kg feed combined with 125 mg vitC/kg feed.Research results showed that the highest WG(Weight Gain)was in E125C125(30.32 g)and lowest in E125C75(17.37 g).There were no statistically significant differences between treatments in terms of laying rate,feed conversion ratio,egg mass and egg weight.However,hatching rate and bodyweight of quails at 1 day of age were significantly different between treatments,the highest was E125C125(74.37%and 7.43 g),followed by E125C75(70.02%and 7.03 g)and the lowest was control(65.89%and 6.82 g).It can be concluded that E125C125 not only improved laying rate but also increased hatching rate and bodyweight of quail chick.
