A developmentally retarded mutant (drm1) was identified from ethyl methanesulfonate (EMS)-mutagenized M2 seedsin Columbia (Col-0) genetic background. The drm1 flowers 109 d after sowing, with a whole life cycle of abo...A developmentally retarded mutant (drm1) was identified from ethyl methanesulfonate (EMS)-mutagenized M2 seedsin Columbia (Col-0) genetic background. The drm1 flowers 109 d after sowing, with a whole life cycle of about 160 d.It also shows a pleiotropic phenotype, e.g., slow germination and lower germination rate, lower growth rate, curlingleaves and abnormal floral organs. The drm1 mutation was a single recessive nuclear mutation, which was mapped tothe bottom of chromosome 5 and located within a region of 20-30 kb around MXK3.1. There have been no mutantswith similar phenotypes reported in the literature, suggesting that DRM1 is a novel flowering promoting locus. Thefindings that the drm1 flowered lately under all photoperiod conditions and its late flowering phenotype was significantlyrestored by vernalization treatment suggest that the drm1 is a typical late flowering mutant and most likely associatedwith the autonomous flowering pathway. The conclusion was further confirmed by the revelation that the transcriptlevel of FLC was constantly upregulated in the drm1 at all the developmental phases examined, except for a very earlystage. Moreover, the transcript levels of two other important repressors, EMF and TFL1, were also upregulated in thedrm1, implying that the two repressors, along with FLC, seems to act in parallel pathways in the drm1 to regulateflowering as well as other aspects of floral development in a negatively additive way. This helps to explain why the drm1exhibits a much more severe late-flowering phenotype than most late-flowering mutants reported. It also implies that theDRM1 might act upstream of these repressors.展开更多
在植物的生命周期中从营养生长到开花是发育过程的重要转折。花启动的时机对生殖生长的成功至关重要。植物开花时间突变体的获得,在揭示植物花发育的奥秘中起了十分重要的作用。本研究以模式植物拟南芥(A rabid op sis tha liana)为材料...在植物的生命周期中从营养生长到开花是发育过程的重要转折。花启动的时机对生殖生长的成功至关重要。植物开花时间突变体的获得,在揭示植物花发育的奥秘中起了十分重要的作用。本研究以模式植物拟南芥(A rabid op sis tha liana)为材料,将野生型拟南芥(Co lum b ia生态型)的种子用甲基黄酸乙酯(EM S)诱变处理,将经诱变处理的种子M1播种收获M2种子用于突变体筛选。以初生莲座叶片数作为筛选指标,筛选出一株晚花突变体,命名flx(flow ering locus x)。展开更多
利用6条ISSR引物对内蒙古西伯利亚杏资源圃内选择的30个早花个体和37个晚花个体进行遗传多样性分析,共扩增出94个位点,其中多态性位点为92个。早花群体等位基因数(Na)为1.8936,有效等位基因数(Ne)为1.4579,多态性位点数为89.36%,Nei...利用6条ISSR引物对内蒙古西伯利亚杏资源圃内选择的30个早花个体和37个晚花个体进行遗传多样性分析,共扩增出94个位点,其中多态性位点为92个。早花群体等位基因数(Na)为1.8936,有效等位基因数(Ne)为1.4579,多态性位点数为89.36%,Nei's基因多样性指数(H)为0.2716,Shannon多样性指数(I)为0.4148。晚花群体依次Na为1.9681,Ne为1.4594,PPB%为96.81%,H为0.2795,I为0.4306。西伯利亚杏晚花群体比早花群体具有更高的遗传多样性。早晚花群体间的遗传分化系数(Gst)为0.0308,总遗传变异的3.08%存在于群体间,96.92%存在于群体内,AMOVA分析也得到了相似结果,群体间的遗传变异是7%,群体内的变异为93%。早晚花群体间的基因流(Nm)为15.7585,群体间有很强的基因流。用NTSYS聚类软件对早晚花67个体进行了聚类,在相似系数0.652处聚成了晚花和早花两类;用Gen Al Ex软件进行主成分分析时,也聚成了晚花和早花两类。研究结果表明,西伯利亚杏晚花群体比早花群体具有更高的遗传多样性,早晚花群体间存在一定的遗传分化。展开更多
文摘A developmentally retarded mutant (drm1) was identified from ethyl methanesulfonate (EMS)-mutagenized M2 seedsin Columbia (Col-0) genetic background. The drm1 flowers 109 d after sowing, with a whole life cycle of about 160 d.It also shows a pleiotropic phenotype, e.g., slow germination and lower germination rate, lower growth rate, curlingleaves and abnormal floral organs. The drm1 mutation was a single recessive nuclear mutation, which was mapped tothe bottom of chromosome 5 and located within a region of 20-30 kb around MXK3.1. There have been no mutantswith similar phenotypes reported in the literature, suggesting that DRM1 is a novel flowering promoting locus. Thefindings that the drm1 flowered lately under all photoperiod conditions and its late flowering phenotype was significantlyrestored by vernalization treatment suggest that the drm1 is a typical late flowering mutant and most likely associatedwith the autonomous flowering pathway. The conclusion was further confirmed by the revelation that the transcriptlevel of FLC was constantly upregulated in the drm1 at all the developmental phases examined, except for a very earlystage. Moreover, the transcript levels of two other important repressors, EMF and TFL1, were also upregulated in thedrm1, implying that the two repressors, along with FLC, seems to act in parallel pathways in the drm1 to regulateflowering as well as other aspects of floral development in a negatively additive way. This helps to explain why the drm1exhibits a much more severe late-flowering phenotype than most late-flowering mutants reported. It also implies that theDRM1 might act upstream of these repressors.
文摘在植物的生命周期中从营养生长到开花是发育过程的重要转折。花启动的时机对生殖生长的成功至关重要。植物开花时间突变体的获得,在揭示植物花发育的奥秘中起了十分重要的作用。本研究以模式植物拟南芥(A rabid op sis tha liana)为材料,将野生型拟南芥(Co lum b ia生态型)的种子用甲基黄酸乙酯(EM S)诱变处理,将经诱变处理的种子M1播种收获M2种子用于突变体筛选。以初生莲座叶片数作为筛选指标,筛选出一株晚花突变体,命名flx(flow ering locus x)。
文摘利用6条ISSR引物对内蒙古西伯利亚杏资源圃内选择的30个早花个体和37个晚花个体进行遗传多样性分析,共扩增出94个位点,其中多态性位点为92个。早花群体等位基因数(Na)为1.8936,有效等位基因数(Ne)为1.4579,多态性位点数为89.36%,Nei's基因多样性指数(H)为0.2716,Shannon多样性指数(I)为0.4148。晚花群体依次Na为1.9681,Ne为1.4594,PPB%为96.81%,H为0.2795,I为0.4306。西伯利亚杏晚花群体比早花群体具有更高的遗传多样性。早晚花群体间的遗传分化系数(Gst)为0.0308,总遗传变异的3.08%存在于群体间,96.92%存在于群体内,AMOVA分析也得到了相似结果,群体间的遗传变异是7%,群体内的变异为93%。早晚花群体间的基因流(Nm)为15.7585,群体间有很强的基因流。用NTSYS聚类软件对早晚花67个体进行了聚类,在相似系数0.652处聚成了晚花和早花两类;用Gen Al Ex软件进行主成分分析时,也聚成了晚花和早花两类。研究结果表明,西伯利亚杏晚花群体比早花群体具有更高的遗传多样性,早晚花群体间存在一定的遗传分化。
