本实验研究了慢性氟中毒对大鼠泌乳及垂体催乳素(Prolactin,PRL)细胞功能和超微结构的影响。结果为:1,慢性氟中毒抑制大鼠乳汁分泌,表现为仔鼠生长缓慢和30 min 平均吮得乳量降低。多巴胺受体阻断剂灭吐灵(Metoclopramide)可改善其泌乳...本实验研究了慢性氟中毒对大鼠泌乳及垂体催乳素(Prolactin,PRL)细胞功能和超微结构的影响。结果为:1,慢性氟中毒抑制大鼠乳汁分泌,表现为仔鼠生长缓慢和30 min 平均吮得乳量降低。多巴胺受体阻断剂灭吐灵(Metoclopramide)可改善其泌乳功能 2,慢性氟中毒授乳鼠血清 PRL 水平降低而垂体内含量升高。电镜下观察到 PRL 细胞质内大的成熟分泌颗粒增多及出现特大异形颗粒,表明可能存在激素释放障碍。上述结果提示慢性氟中毒可抑制大鼠的乳汁分泌,氟化物的毒性作用可能由下丘脑多巴胺系统活动的增强所中介。展开更多
Tannic acid has been employed to capture the exocytotic secretory granules by binding amino and carboxyl groups of secreted proteins and mordanting them into eletrondense material, and making them easily observed and ...Tannic acid has been employed to capture the exocytotic secretory granules by binding amino and carboxyl groups of secreted proteins and mordanting them into eletrondense material, and making them easily observed and precisely quantified by electron microscopy. The present study, using tannic acid-captured exocytotic method,aims to investigate ultrastructurally the secretion of the anterior pituitary gland cells of the rat. The exocytosis is frequently identified on lactotrophs and corticotrophs, but rarely somatotrophs,gonadotrophs and thyrotrophs. 12 lactotrophs and 12 corticotrophs of the anterior pituitary are randomly selected from each sample and the exocytotic secretory granules (ESG) are counted. The ESG(x) is 1. 1 6?. 1 6 in lactotrophs and 1. 21?0.24 in corticotrophs (P>0. 05). The results morphologically suggest that the lactotrophs,as well as corticotrophs strongly respond to the stress.展开更多
文摘本实验研究了慢性氟中毒对大鼠泌乳及垂体催乳素(Prolactin,PRL)细胞功能和超微结构的影响。结果为:1,慢性氟中毒抑制大鼠乳汁分泌,表现为仔鼠生长缓慢和30 min 平均吮得乳量降低。多巴胺受体阻断剂灭吐灵(Metoclopramide)可改善其泌乳功能 2,慢性氟中毒授乳鼠血清 PRL 水平降低而垂体内含量升高。电镜下观察到 PRL 细胞质内大的成熟分泌颗粒增多及出现特大异形颗粒,表明可能存在激素释放障碍。上述结果提示慢性氟中毒可抑制大鼠的乳汁分泌,氟化物的毒性作用可能由下丘脑多巴胺系统活动的增强所中介。
文摘Tannic acid has been employed to capture the exocytotic secretory granules by binding amino and carboxyl groups of secreted proteins and mordanting them into eletrondense material, and making them easily observed and precisely quantified by electron microscopy. The present study, using tannic acid-captured exocytotic method,aims to investigate ultrastructurally the secretion of the anterior pituitary gland cells of the rat. The exocytosis is frequently identified on lactotrophs and corticotrophs, but rarely somatotrophs,gonadotrophs and thyrotrophs. 12 lactotrophs and 12 corticotrophs of the anterior pituitary are randomly selected from each sample and the exocytotic secretory granules (ESG) are counted. The ESG(x) is 1. 1 6?. 1 6 in lactotrophs and 1. 21?0.24 in corticotrophs (P>0. 05). The results morphologically suggest that the lactotrophs,as well as corticotrophs strongly respond to the stress.
