Spherical Ag nanoparticles(AgNPs) with a diameter of 20 nm or smaller were biologically synthesized using algae Parachlorella kessleri. The effect of storage conditions on the long-term stability of AgNPs was investig...Spherical Ag nanoparticles(AgNPs) with a diameter of 20 nm or smaller were biologically synthesized using algae Parachlorella kessleri. The effect of storage conditions on the long-term stability of AgNPs was investigated. UV/Vis spectrophotometry, transmission electron microscopy, and dynamic light scattering measurements revealed that the long-term stability of AgNPs was influenced by light and temperature conditions. The most significant loss of stability was observed for the AgNPs stored in daylight at room temperature. The AgNPs stored under these conditions began to lose their stability after approximately 30 d; after 100 d, a substantial amount of agglomerated particles settled to the bottom of the Erlenmeyer flask. The AgNPs stored in the dark at room temperature exhibited better long-term stability. Weak particle agglomeration began at approximately the 100 th day. The AgNPs stored in the dark at about 5℃ exhibited the best long-term stability; the AgNPs stored under such conditions remained spherical, with a narrow size distribution, and stable(no agglomeration) even after 6 months. Zeta-potential measurements confirmed better dispersity and stability of AgNPs stored under these conditions.展开更多
The induction of biofilm formation has been explored as a means of harvesting microalgae for bioprocessing applications. Environmental stressors have been implicated in the induction of biofilm formation;however, it i...The induction of biofilm formation has been explored as a means of harvesting microalgae for bioprocessing applications. Environmental stressors have been implicated in the induction of biofilm formation;however, it is unclear whether all stressors, or a select few, are responsible. This study aimed to investigate the effects of three stressors, nitrogen depletion, reduced or elongated day lengths, and increased culture turbulence on bio-film formation of Parachlorella kessleri. We also examined corresponding effects on growth and production of reactive oxygen species. Turbulence induced the greatest response in which a significant decrease in growth plus an increase in superoxide production and flocculation efficiency were seen for the 300-rpm treatment. For varying day lengths, stress response was not observed, however, a significant increase in EPS secretion was measured in both shorter and longer days. Nitrogen depletion induced a low-level stress response, in which superoxide production increased for the highest concentrations, while growth was not impacted. In contrast to previous studies on nitrogen depletion, a significant increase in EPS secretion was not observed. Results indicate that stress response varies according to type and magnitude. EPS production and thus biofilm formation are not linked to the stress indicators investigated. P. kessleri uses small quantities of EPS to contribute to cell stickiness, but not necessarily to the full formation of a biofilm;however, cell stickiness served as a mechanism for substrate adherence and cellular aggregation none the less.展开更多
Spherical Ag nanoparticles（AgNPs） were biologically synthesized using four different extracts prepared from Parachlorella kessleri algae cultivated for 1, 2, 3 and 4 weeks. The influence of algae life cycle on AgNPs...Spherical Ag nanoparticles（AgNPs） were biologically synthesized using four different extracts prepared from Parachlorella kessleri algae cultivated for 1, 2, 3 and 4 weeks. The influence of algae life cycle on AgNPs formation and effect of different storage conditions on AgNPs long-term stability were investigated. The age of algae influenced the rate of AgNPs synthesis and amount of AgNPs in solution. The age of algae did not influence the AgNPs long-term stability. UV–vis and TEM observation revealed that long-term stability of AgNPs can be influenced by storage temperatures, and low temperature positively influences the AgNPs stability. AgNPs stored at dark and at temperature of ~5 °C showed the best long-term stability regardless of the culture age. Such AgNPs remained spherical, fine（5-20 nm） and stable（no agglomeration） even after 6 months.展开更多
基金supported by Slovak Grant Agency (VEGA 1/0197/15)the Ministry of Education, Youth and Sport of the Czech Republic within the scope of project No.LO1207 of the program NPU1
文摘Spherical Ag nanoparticles(AgNPs) with a diameter of 20 nm or smaller were biologically synthesized using algae Parachlorella kessleri. The effect of storage conditions on the long-term stability of AgNPs was investigated. UV/Vis spectrophotometry, transmission electron microscopy, and dynamic light scattering measurements revealed that the long-term stability of AgNPs was influenced by light and temperature conditions. The most significant loss of stability was observed for the AgNPs stored in daylight at room temperature. The AgNPs stored under these conditions began to lose their stability after approximately 30 d; after 100 d, a substantial amount of agglomerated particles settled to the bottom of the Erlenmeyer flask. The AgNPs stored in the dark at room temperature exhibited better long-term stability. Weak particle agglomeration began at approximately the 100 th day. The AgNPs stored in the dark at about 5℃ exhibited the best long-term stability; the AgNPs stored under such conditions remained spherical, with a narrow size distribution, and stable(no agglomeration) even after 6 months. Zeta-potential measurements confirmed better dispersity and stability of AgNPs stored under these conditions.
文摘The induction of biofilm formation has been explored as a means of harvesting microalgae for bioprocessing applications. Environmental stressors have been implicated in the induction of biofilm formation;however, it is unclear whether all stressors, or a select few, are responsible. This study aimed to investigate the effects of three stressors, nitrogen depletion, reduced or elongated day lengths, and increased culture turbulence on bio-film formation of Parachlorella kessleri. We also examined corresponding effects on growth and production of reactive oxygen species. Turbulence induced the greatest response in which a significant decrease in growth plus an increase in superoxide production and flocculation efficiency were seen for the 300-rpm treatment. For varying day lengths, stress response was not observed, however, a significant increase in EPS secretion was measured in both shorter and longer days. Nitrogen depletion induced a low-level stress response, in which superoxide production increased for the highest concentrations, while growth was not impacted. In contrast to previous studies on nitrogen depletion, a significant increase in EPS secretion was not observed. Results indicate that stress response varies according to type and magnitude. EPS production and thus biofilm formation are not linked to the stress indicators investigated. P. kessleri uses small quantities of EPS to contribute to cell stickiness, but not necessarily to the full formation of a biofilm;however, cell stickiness served as a mechanism for substrate adherence and cellular aggregation none the less.
基金supported by Slovak Grant Agency(VEGA 1/0197/15)the Ministry of Education,Youth and Sport of the Czech Republic within the scope of project No.LO1207 of the programme NPU1
文摘Spherical Ag nanoparticles（AgNPs） were biologically synthesized using four different extracts prepared from Parachlorella kessleri algae cultivated for 1, 2, 3 and 4 weeks. The influence of algae life cycle on AgNPs formation and effect of different storage conditions on AgNPs long-term stability were investigated. The age of algae influenced the rate of AgNPs synthesis and amount of AgNPs in solution. The age of algae did not influence the AgNPs long-term stability. UV–vis and TEM observation revealed that long-term stability of AgNPs can be influenced by storage temperatures, and low temperature positively influences the AgNPs stability. AgNPs stored at dark and at temperature of ~5 °C showed the best long-term stability regardless of the culture age. Such AgNPs remained spherical, fine（5-20 nm） and stable（no agglomeration） even after 6 months.
