<div style="text-align:justify;"> We developed a Bessel-beam photoacoustic microscopical simulation platform by using the k-Wave: MATLAB toolbox. The simulation platform uses the ring slit method to ge...<div style="text-align:justify;"> We developed a Bessel-beam photoacoustic microscopical simulation platform by using the k-Wave: MATLAB toolbox. The simulation platform uses the ring slit method to generate Bessel beam. By controlling the inner and outer radius of the ring slit, the depth-of-field (DoF) of Bessel beam can be controlled. And the large volumetric image is obtained by point scanning. The simulation experiments on blood vessels were carried out to demonstrate the feasibility of the simulation platform. This simulation work can be used as an auxiliary tool for the research of Bessel-beam photoacoustic microscopy. </div>展开更多
<div style="text-align:justify;"> Photoacoustic imaging has emerged in the past decades. Compared with the traditional medical imaging mode, it has better imaging performance and has great development ...<div style="text-align:justify;"> Photoacoustic imaging has emerged in the past decades. Compared with the traditional medical imaging mode, it has better imaging performance and has great development potential in the field of biological imaging. In traditional photoacoustic microscopy, a single laser pulse is generally used to irradiate the sample to produce photoacoustic signal. And sig-nal-to-noise ratio (SNR) is a very important indicator for photoacoustic im-aging. In order to obtain the image with high SNR, multiple acquisition or increasing laser pulse energy is usually adopted. The former will lead to slower imaging speed, and the latter will lead to photobleaching or pho-totoxicity. Here, we propose multi-pulse photoacoustic microscopy, the photoacoustic signals were stimulated sequentially using multiple laser pulses in each A line data acquisition. In order to verify the feasibility of this method, a multi-pulse photoacoustic imaging simulation platform is established using k-Wave toolbox. The performance of multi-pulse photo-acoustic imaging is verified through the three scanning modes of photoa-coustic microscopy A-scan, B-scan, and C-scan. The results indicate that the SNR is proportion to the number of laser pulses used, high SNR can be achieved by low-energy laser pulse. This work will help to expand the ap-plication of photoacoustic imaging. </div>展开更多
文摘<div style="text-align:justify;"> We developed a Bessel-beam photoacoustic microscopical simulation platform by using the k-Wave: MATLAB toolbox. The simulation platform uses the ring slit method to generate Bessel beam. By controlling the inner and outer radius of the ring slit, the depth-of-field (DoF) of Bessel beam can be controlled. And the large volumetric image is obtained by point scanning. The simulation experiments on blood vessels were carried out to demonstrate the feasibility of the simulation platform. This simulation work can be used as an auxiliary tool for the research of Bessel-beam photoacoustic microscopy. </div>
文摘<div style="text-align:justify;"> Photoacoustic imaging has emerged in the past decades. Compared with the traditional medical imaging mode, it has better imaging performance and has great development potential in the field of biological imaging. In traditional photoacoustic microscopy, a single laser pulse is generally used to irradiate the sample to produce photoacoustic signal. And sig-nal-to-noise ratio (SNR) is a very important indicator for photoacoustic im-aging. In order to obtain the image with high SNR, multiple acquisition or increasing laser pulse energy is usually adopted. The former will lead to slower imaging speed, and the latter will lead to photobleaching or pho-totoxicity. Here, we propose multi-pulse photoacoustic microscopy, the photoacoustic signals were stimulated sequentially using multiple laser pulses in each A line data acquisition. In order to verify the feasibility of this method, a multi-pulse photoacoustic imaging simulation platform is established using k-Wave toolbox. The performance of multi-pulse photo-acoustic imaging is verified through the three scanning modes of photoa-coustic microscopy A-scan, B-scan, and C-scan. The results indicate that the SNR is proportion to the number of laser pulses used, high SNR can be achieved by low-energy laser pulse. This work will help to expand the ap-plication of photoacoustic imaging. </div>
