Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophil...Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp. Results: A total of 432 ticks were collected: 30(6.94%) Haemaphysalis punctata, 72(16.7%) Dermacentor marginatus and 330(76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58(75.32%) resulted infected and among them 14(18.18%) showed co-infections. In particular, 29(37.66%) pools were positive for Bartonella spp., 23(29.87%) for Anaplasma phagocytophilum, 16(20.78%) for Rickettsia spp., and 5(6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii. Conclusions: The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.展开更多
Identification of lxodid tick species on livestock in two districts, viz., D.I. Khan and Lakki Marwat of Northern Pakistan were made during the period from March to November 2009 through animals' survey and questionn...Identification of lxodid tick species on livestock in two districts, viz., D.I. Khan and Lakki Marwat of Northern Pakistan were made during the period from March to November 2009 through animals' survey and questionnaire. The main tick species were Amblyomma (14.3%), Boophilus microplus (l 8.5%) and Hyalomma anatolicum and H. dromedarii (67.2%). Out of 3009 collected ticks, the highest number of female (88.7%) and the lowest of males ( l 1.3%) belong to Boophilus; the lowest number of female (92.4%) and the highest of males (77.5%) belong to Amblyomma. However, cows were more susceptible than buffaloes and camels ranked third to ticks infestation. Moreover, cows, buffalos, goats and sheep harbored mixed infestation (had more than one type of ticks), however, camels and donkey harbored single infestation (had only one type of tick). An emerging ethno-veterinary or traditional remedy is poultry keeping for ticks' elimination in Lakki Marwat. The results of this research will be helped in taking appropriate measures to reduce tick infestation and to improve management practices. Hence, it will help to increase farm productivity and farmers income.展开更多
文摘Objective: To determine the prevalence of zoonotic tick-borne bacteria in feeding ticks removed from hunted wild animals. Methods: PCR was executed on DNA extracted from 77 tick pools to detect Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi sensu lato, Coxiella burnetii and Rickettsia spp. Results: A total of 432 ticks were collected: 30(6.94%) Haemaphysalis punctata, 72(16.7%) Dermacentor marginatus and 330(76.38%) Ixodes ricinus. For each animal one or two pools of 3 ticks of the same species was constituted. Seventy-seven tick pools were examined by PCR: 58(75.32%) resulted infected and among them 14(18.18%) showed co-infections. In particular, 29(37.66%) pools were positive for Bartonella spp., 23(29.87%) for Anaplasma phagocytophilum, 16(20.78%) for Rickettsia spp., and 5(6.49%) for Borrelia burgdorferi s.l. All samples were negative for Coxiella burnetii. Conclusions: The results demonstrate the presence of several zoonotic tick-borne pathogens in the studied area, and underline the risk of exposure to infections for hunters not only during the outdoor activity, but also when they manipulate hunted animals infested by infected ticks.
文摘Identification of lxodid tick species on livestock in two districts, viz., D.I. Khan and Lakki Marwat of Northern Pakistan were made during the period from March to November 2009 through animals' survey and questionnaire. The main tick species were Amblyomma (14.3%), Boophilus microplus (l 8.5%) and Hyalomma anatolicum and H. dromedarii (67.2%). Out of 3009 collected ticks, the highest number of female (88.7%) and the lowest of males ( l 1.3%) belong to Boophilus; the lowest number of female (92.4%) and the highest of males (77.5%) belong to Amblyomma. However, cows were more susceptible than buffaloes and camels ranked third to ticks infestation. Moreover, cows, buffalos, goats and sheep harbored mixed infestation (had more than one type of ticks), however, camels and donkey harbored single infestation (had only one type of tick). An emerging ethno-veterinary or traditional remedy is poultry keeping for ticks' elimination in Lakki Marwat. The results of this research will be helped in taking appropriate measures to reduce tick infestation and to improve management practices. Hence, it will help to increase farm productivity and farmers income.