Objective To analyze the factors relating with fertilization failure in conventional IVF cycles. Methods The total fertilization failure rate of 2 429 IVF cycles from January 2011 to May 2012 in the Reproductive Centr...Objective To analyze the factors relating with fertilization failure in conventional IVF cycles. Methods The total fertilization failure rate of 2 429 IVF cycles from January 2011 to May 2012 in the Reproductive Centre of the Women and Children Hospital of Jiangxi Province were retrospectively analyzed. Risk factors were identified by univariate and multivariate logistic regression analyses. Results The total fertilization failure rate of conventional IVF-ET rate was 5. 7% （139/ 2 429）. Thepercentage of morphologically normal sperm （11.1 ±5.8% vs 13.4 ±5.3%）, progressive motility （47.4± 10.5% vs 50.1 ±8.6%）, percentage of couple primary infertility （79.1% vs 44.3%）, percentage o f female primary infertility （69.1% vs 36.3%）, percentage of male primary infertility （74.8% vs 41.7%）, percentage of without oviduct obstruction patients （30.2% vs 13.6%） and percentage of couples primary infertility （79. 1% vs 44. 3%） of the fertilization failure patients were significantly different from those of the fertilized patients （P〈O.05）. Besides, higher total fertilization failure rate was found in couples with isolated teratozoospermia than in couples with normal percentage of morphologically normal sperm （15.0% vs 5.2%）. After that, it was found by multivariate logistic regression analyses that many factors including percentage of morphologically normal sperm, sperm concentration, couples primary infertility and female infertility years were related with fertilization failure. Conclusion Patients with low percentage of morphologically normal sperm （〈4%）, low sperm concentration and motility, male primary infertility, female primary infertility, without oviduct obstruction, long female infertility years or/and couples primary infertility are at high risk of fertilization failure. More attention should be paid to these patients for avoiding the fertilization failure.展开更多
Objective To determine whether patients with isolated teratozoospermia have increased or decreased incidence of chromosomal aneuploidies. Methods Sperm obtained from isolated teratozoospermic men （teratozoospermic gr...Objective To determine whether patients with isolated teratozoospermia have increased or decreased incidence of chromosomal aneuploidies. Methods Sperm obtained from isolated teratozoospermic men （teratozoospermic group, n=18） and normal fertile men （the control, n=5） were analyzed using FISH （for chromosomes 18, X and Y）. Results A total of 58 178 spermatozoa were counted from the teratozoospermia group and 16 369 spermatozoa were counted from the control, with the hybridization rates of 97.5% and 98.3%, respectively. The major types of chromosomal aneuploidies were disomy （YY18, XX18, XY18, Y1818 and 3（1818） and diploidy （1818XX, 1818YY, 1818XY）. In the teratozoospermic group and the control, the disomy rates of 18 chromosome were 0.29 ±0.16% and 0.03 ±0.02%, the disomy rates of sex chromo- some were 0.65 ±0.24% and 0.05 ± 0.02%, the diploidy rates were 0.14 ± 0.12% and 0. 04±0.03%, respectively..411 the differences between these two groups were significant （P〈0. 05）.Conclusion Sperm of isolated teratozoospermic men have higher rates of 18, X and Y chromosomal aneuploidies than that of the fertile controls.展开更多
基金funded by the Key Project of Jiangxi Provincial Department of Public Health,No.20104010
文摘Objective To analyze the factors relating with fertilization failure in conventional IVF cycles. Methods The total fertilization failure rate of 2 429 IVF cycles from January 2011 to May 2012 in the Reproductive Centre of the Women and Children Hospital of Jiangxi Province were retrospectively analyzed. Risk factors were identified by univariate and multivariate logistic regression analyses. Results The total fertilization failure rate of conventional IVF-ET rate was 5. 7% （139/ 2 429）. Thepercentage of morphologically normal sperm （11.1 ±5.8% vs 13.4 ±5.3%）, progressive motility （47.4± 10.5% vs 50.1 ±8.6%）, percentage of couple primary infertility （79.1% vs 44.3%）, percentage o f female primary infertility （69.1% vs 36.3%）, percentage of male primary infertility （74.8% vs 41.7%）, percentage of without oviduct obstruction patients （30.2% vs 13.6%） and percentage of couples primary infertility （79. 1% vs 44. 3%） of the fertilization failure patients were significantly different from those of the fertilized patients （P〈O.05）. Besides, higher total fertilization failure rate was found in couples with isolated teratozoospermia than in couples with normal percentage of morphologically normal sperm （15.0% vs 5.2%）. After that, it was found by multivariate logistic regression analyses that many factors including percentage of morphologically normal sperm, sperm concentration, couples primary infertility and female infertility years were related with fertilization failure. Conclusion Patients with low percentage of morphologically normal sperm （〈4%）, low sperm concentration and motility, male primary infertility, female primary infertility, without oviduct obstruction, long female infertility years or/and couples primary infertility are at high risk of fertilization failure. More attention should be paid to these patients for avoiding the fertilization failure.
基金funded by the Key Project of Jiangxi Provincial Department of Public Health, No. 20104010
文摘Objective To determine whether patients with isolated teratozoospermia have increased or decreased incidence of chromosomal aneuploidies. Methods Sperm obtained from isolated teratozoospermic men （teratozoospermic group, n=18） and normal fertile men （the control, n=5） were analyzed using FISH （for chromosomes 18, X and Y）. Results A total of 58 178 spermatozoa were counted from the teratozoospermia group and 16 369 spermatozoa were counted from the control, with the hybridization rates of 97.5% and 98.3%, respectively. The major types of chromosomal aneuploidies were disomy （YY18, XX18, XY18, Y1818 and 3（1818） and diploidy （1818XX, 1818YY, 1818XY）. In the teratozoospermic group and the control, the disomy rates of 18 chromosome were 0.29 ±0.16% and 0.03 ±0.02%, the disomy rates of sex chromo- some were 0.65 ±0.24% and 0.05 ± 0.02%, the diploidy rates were 0.14 ± 0.12% and 0. 04±0.03%, respectively..411 the differences between these two groups were significant （P〈0. 05）.Conclusion Sperm of isolated teratozoospermic men have higher rates of 18, X and Y chromosomal aneuploidies than that of the fertile controls.
