OBJECTIVE: To observe the level of muscarinic receptors in airway and lung tissues, and the effect of inhaled ipratropium bromide on these receptors in a rat model of chronic obstructive pulmonary disease (COPD). METH...OBJECTIVE: To observe the level of muscarinic receptors in airway and lung tissues, and the effect of inhaled ipratropium bromide on these receptors in a rat model of chronic obstructive pulmonary disease (COPD). METHODS: This model was developed by exposure of rats to 250 ppm SO2 gas, 5 h/d, 5 d/wk, for a period of 7 wk. The COPD rats inhaled 0.025% aerosolized iratropium bromide for 20 min, 2 times daily, in an airtight chamber. Muscarinic receptors in airway and lung tissues of normal rats, ipratropium bromide-treated COPD rats and the recovering COPD rats were measured by the radio-ligand binding assay. RESULTS: Airway/lung pathology and pulmonary function tests showed that chronic SO2 exposure caused pathophysiologic changes similar to those observed in human COPD. The density (0.038 +/- 0.011, pmol/mg protein) and affinity (Kd, 23 +/- 11 pmol/L) of muscarinic receptors in airway and lung tissues of COPD rats were not changed compared with those of normal control rats (0.030 +/- 0.008 and 29 +/- 19, respectively, P > 0.05). Densities of the muscarinic receptors were not changed after inhalation of ipratropium bromide for 5 days, but increased significantly after inhalation for 30 days, as compared with those of the untreated COPD rats. The muscarinic receptors returned the normal levels at day 6 after cessation of ipratropium bromide treatment. There were no differences among different groups of rats in equilibrium dissociation constants (Kd). CONCLUSION: A rat model of COPD with pathophysiologic changes similar to the human counterpart was developed using chronic SO2 exposure. There was no significant change in the number and function of muscarinic receptors in airway and lung tissues of the COPD rats, but upregulation of the muscarinic receptors was observed after long-term inhalation of ipratropium bromide.展开更多
目的:分析胸腔镜下老年肺癌手术患者围手术期应用爱全乐雾化吸入联合沐舒坦静脉滴注对动脉血气指标、肺功能及炎性因子的影响。方法:选取本院2015年8月至2017年8月择期开展胸腔镜手术治疗的老年(>60岁)肺癌病患72例,采用随机数字表...目的:分析胸腔镜下老年肺癌手术患者围手术期应用爱全乐雾化吸入联合沐舒坦静脉滴注对动脉血气指标、肺功能及炎性因子的影响。方法:选取本院2015年8月至2017年8月择期开展胸腔镜手术治疗的老年(>60岁)肺癌病患72例,采用随机数字表法将患者分为观察组和对照组两组,各36例,观察组围手术期给予爱全乐雾化吸入联合沐舒坦静脉滴注治疗,对照组围手术期给予等量生理盐水补充。对比两组术前、术后5 d动脉血气[动脉氧分压(PaO2)、血酸碱值(pH)、氧饱和度(SaO2)、动脉血二氧化碳分压(PaCO2)],肺功能[第1秒用力呼气量(FEV1)、每分钟最大通气量(MVV)、第1秒用力呼气量占用力肺活量比值(FEV1/FVC)],炎性因子[肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、C反应蛋白(CRP)],记录呼吸系统并发症。结果:观察组术后机械通气时间、ICU观察时间、住院时间以及术后5 d的痰液性质、痰液性状及咳嗽难易度均明显低于对照组(P<0.05)。观察组术后5 d PaO2、FEV1、MVV、FEV1/FVC水平均明显高于对照组(P<0.05)。观察组术后5 d TNF-α、IL-6、CRP水平及呼吸系统并发症、ICU再入率显著低于对照组(P<0.05)。结论:实施爱全乐雾化吸入联合沐舒坦静脉滴注对胸腔镜下老年肺癌手术患者围手术期干预可有效改善患者肺功能、促进动脉血氧恢复、减轻炎症反应、减少术后并发症。展开更多
文摘OBJECTIVE: To observe the level of muscarinic receptors in airway and lung tissues, and the effect of inhaled ipratropium bromide on these receptors in a rat model of chronic obstructive pulmonary disease (COPD). METHODS: This model was developed by exposure of rats to 250 ppm SO2 gas, 5 h/d, 5 d/wk, for a period of 7 wk. The COPD rats inhaled 0.025% aerosolized iratropium bromide for 20 min, 2 times daily, in an airtight chamber. Muscarinic receptors in airway and lung tissues of normal rats, ipratropium bromide-treated COPD rats and the recovering COPD rats were measured by the radio-ligand binding assay. RESULTS: Airway/lung pathology and pulmonary function tests showed that chronic SO2 exposure caused pathophysiologic changes similar to those observed in human COPD. The density (0.038 +/- 0.011, pmol/mg protein) and affinity (Kd, 23 +/- 11 pmol/L) of muscarinic receptors in airway and lung tissues of COPD rats were not changed compared with those of normal control rats (0.030 +/- 0.008 and 29 +/- 19, respectively, P > 0.05). Densities of the muscarinic receptors were not changed after inhalation of ipratropium bromide for 5 days, but increased significantly after inhalation for 30 days, as compared with those of the untreated COPD rats. The muscarinic receptors returned the normal levels at day 6 after cessation of ipratropium bromide treatment. There were no differences among different groups of rats in equilibrium dissociation constants (Kd). CONCLUSION: A rat model of COPD with pathophysiologic changes similar to the human counterpart was developed using chronic SO2 exposure. There was no significant change in the number and function of muscarinic receptors in airway and lung tissues of the COPD rats, but upregulation of the muscarinic receptors was observed after long-term inhalation of ipratropium bromide.
文摘目的:分析胸腔镜下老年肺癌手术患者围手术期应用爱全乐雾化吸入联合沐舒坦静脉滴注对动脉血气指标、肺功能及炎性因子的影响。方法:选取本院2015年8月至2017年8月择期开展胸腔镜手术治疗的老年(>60岁)肺癌病患72例,采用随机数字表法将患者分为观察组和对照组两组,各36例,观察组围手术期给予爱全乐雾化吸入联合沐舒坦静脉滴注治疗,对照组围手术期给予等量生理盐水补充。对比两组术前、术后5 d动脉血气[动脉氧分压(PaO2)、血酸碱值(pH)、氧饱和度(SaO2)、动脉血二氧化碳分压(PaCO2)],肺功能[第1秒用力呼气量(FEV1)、每分钟最大通气量(MVV)、第1秒用力呼气量占用力肺活量比值(FEV1/FVC)],炎性因子[肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、C反应蛋白(CRP)],记录呼吸系统并发症。结果:观察组术后机械通气时间、ICU观察时间、住院时间以及术后5 d的痰液性质、痰液性状及咳嗽难易度均明显低于对照组(P<0.05)。观察组术后5 d PaO2、FEV1、MVV、FEV1/FVC水平均明显高于对照组(P<0.05)。观察组术后5 d TNF-α、IL-6、CRP水平及呼吸系统并发症、ICU再入率显著低于对照组(P<0.05)。结论:实施爱全乐雾化吸入联合沐舒坦静脉滴注对胸腔镜下老年肺癌手术患者围手术期干预可有效改善患者肺功能、促进动脉血氧恢复、减轻炎症反应、减少术后并发症。
