AIM： To explore the relationship between changes of intestinal environment and pathogenesis of non-alcoholic steatohepatitis （NASH）. METHODS： Forty-two Sprague-Dawley rats were randomly dMded into model group （n ...AIM： To explore the relationship between changes of intestinal environment and pathogenesis of non-alcoholic steatohepatitis （NASH）. METHODS： Forty-two Sprague-Dawley rats were randomly dMded into model group （n = 24）, treatment group （n = 12）, and control group （n = 6）. The rats of model and treatment groups were given high-fat diet, and those of the control group were given normal diet. Furthermore, the rats of treatment group were given lactulose after 8 wk of high-fat diet. Twelve rats of the model group were killed at 8 wk of high-fat diet. At the 16 wk the rats of treatment group, control group, and the rest of the model group were killed. The serum levels of aminotransferase were measured and the histology of livers was observed by H＆E staining. RESULTS： The livers of rats presented the pathological features of steatohepatitis with higher serum levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in the model group after 16 wk. Compared to the model group, the serum levels of ALT and AST in treatment group decreased significantly and were close to the normal group, and the hepatic inflammation scores also decreased markedly than those in the model group after 16 wk （5.83±2.02 vs 3.63±0.64, P〈0.05）, but were still higher than those in the model group after 8 wk （3.63±0.64 vs 1.98±0.90, P〈0.05）. However, the degree of hepatic steatosis had no changes in treatment group compared to the model group after 16 wk. CONCLUSION： Lactulose could ameliorate the hepatic inflammation of rats with steatohepatitis induced by fat- rich diet, but could not completely prevent the development of steatohepatitis. It is suggested that intestinal environmental changes such as intestinal bacteria overgrowth, are one of the important factors in the pathogenesis of NASH.展开更多
The human gastrointestinal tract accommodates an entire micro-environment for divergent physiologic processes,the dysbiosis of this micro-ecology has a strong inter-action with the pathogenesis of inflammatory bowel d...The human gastrointestinal tract accommodates an entire micro-environment for divergent physiologic processes,the dysbiosis of this micro-ecology has a strong inter-action with the pathogenesis of inflammatory bowel disease(IBD).In the past few years,with the advances in the understanding of microbiome,its metabolites and further application of next generation sequencing,analysis of dynamic alteration of gut micro-environment was realized,which provides numerous information beyond simple microbiota structure or metabolites differences under chronic colitis status.The subsequent intervention strategies targeting the modulation of intestinal micro-environment have been explored as a potential therapy.In this review,we will summarize the recent knowledge about multi-dimensional dysbiosis,the inter-action between fungus and bacteria under inflamed mucosa,and the clinical application of probiotics and fecal microbiota transplantation as a promising therapeutic approach in IBD.展开更多
文摘AIM： To explore the relationship between changes of intestinal environment and pathogenesis of non-alcoholic steatohepatitis （NASH）. METHODS： Forty-two Sprague-Dawley rats were randomly dMded into model group （n = 24）, treatment group （n = 12）, and control group （n = 6）. The rats of model and treatment groups were given high-fat diet, and those of the control group were given normal diet. Furthermore, the rats of treatment group were given lactulose after 8 wk of high-fat diet. Twelve rats of the model group were killed at 8 wk of high-fat diet. At the 16 wk the rats of treatment group, control group, and the rest of the model group were killed. The serum levels of aminotransferase were measured and the histology of livers was observed by H＆E staining. RESULTS： The livers of rats presented the pathological features of steatohepatitis with higher serum levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in the model group after 16 wk. Compared to the model group, the serum levels of ALT and AST in treatment group decreased significantly and were close to the normal group, and the hepatic inflammation scores also decreased markedly than those in the model group after 16 wk （5.83±2.02 vs 3.63±0.64, P〈0.05）, but were still higher than those in the model group after 8 wk （3.63±0.64 vs 1.98±0.90, P〈0.05）. However, the degree of hepatic steatosis had no changes in treatment group compared to the model group after 16 wk. CONCLUSION： Lactulose could ameliorate the hepatic inflammation of rats with steatohepatitis induced by fat- rich diet, but could not completely prevent the development of steatohepatitis. It is suggested that intestinal environmental changes such as intestinal bacteria overgrowth, are one of the important factors in the pathogenesis of NASH.
基金supported by a grant from the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(No.2016-12M-3-001).
文摘The human gastrointestinal tract accommodates an entire micro-environment for divergent physiologic processes,the dysbiosis of this micro-ecology has a strong inter-action with the pathogenesis of inflammatory bowel disease(IBD).In the past few years,with the advances in the understanding of microbiome,its metabolites and further application of next generation sequencing,analysis of dynamic alteration of gut micro-environment was realized,which provides numerous information beyond simple microbiota structure or metabolites differences under chronic colitis status.The subsequent intervention strategies targeting the modulation of intestinal micro-environment have been explored as a potential therapy.In this review,we will summarize the recent knowledge about multi-dimensional dysbiosis,the inter-action between fungus and bacteria under inflamed mucosa,and the clinical application of probiotics and fecal microbiota transplantation as a promising therapeutic approach in IBD.
