Several strategies with different combination of inocula and packing material were investigated to obtain the optimal start-up time and elimination capacity (EC) in toluene biofiltration. The inocula contained the act...Several strategies with different combination of inocula and packing material were investigated to obtain the optimal start-up time and elimination capacity (EC) in toluene biofiltration. The inocula contained the activated sludge and toluene degrading bacteria, and the packing material consisted of different mixing ratios of peat and wood chips. A final toluene load of 21.2 g/(m3·h) was attained step by step in four parallel biofilters. A shortest start-up time of 15 days and a highest EC of 17.0 g/(m3·h) were observed in the biofilter B-4, which was inoculated with a special microbial consortium consisting of three strains of toluene degrading bacteria and was packed with the mixture of peat and wood chips at a ratio of 80:20 (w/w). These results indicated that inoculating pre-acclimatized microbes dramatically shortened the start-up time, and such a composition of packing material could maintain an appropriate environment (with the bed porosity and water content equating to 0.45 and 1.96, respectively) for the growth of dominant toluene degrading bacteria in the biofilter.展开更多
The application of arbuscular mycorrhizal (AM) inocula in severely degraded wetlands could ensure success in restoration.Mycorrhizal fungi play an important role in plant individual's survival and development in a...The application of arbuscular mycorrhizal (AM) inocula in severely degraded wetlands could ensure success in restoration.Mycorrhizal fungi play an important role in plant individual's survival and development in a low nutrient condition.Based on the importance that mycorrhizal fungi have to their host plants,mycorrhizal inocula have been produced and applied in terrestrial ecosystems in order to let the plants become mycorrhizal.However,mycorrhizal inocula have not been used in wetland restorations,despite increasing evidence that mycorrhizal fungi are commonly found in various wetland systems and have the ability to survive under anoxic conditions.Evidence also shows that mycorrhizal fungal inocula in the soil could have been destroyed in the degraded wetland or could be destroyed during traditional wetland restoration process.Therefore,AM inocula production is strongly recommended for wetland restoration.In this paper,I will argue that AM inocula production is required when introduced recovery is necessary,and aeroponic culture technique is a preferable method to produce AM inocula.Last,a renewed wetland restoration flow chart is summarized.展开更多
To demonstrate the feasibility of using bioaugmentation to enhance biodegradation of quinoline, four strains capable of using quinoline as sole source of carbon, nitrogen and energy were isolated from different enviro...To demonstrate the feasibility of using bioaugmentation to enhance biodegradation of quinoline, four strains capable of using quinoline as sole source of carbon, nitrogen and energy were isolated from different environmental samples by enrichment technique. Screening for quinoline degrader with the highest quinoline mineralizing rate was carried out in respirometer and one bacterium identified as \%Burkholderia pickettii W2\% was chosen as inoculum in bioaugmentation tests. Quinoline biodegradation experiment results showed that this bacterium degraded quinoline very quickly. 100, 200 and 500 mg/L quinoline can be transformed completely within 1, 2 and 7 hours respectively. A bioaugmentation procedure was proposed and laboratory experiments confirmed that bioaugmentation was an effective way to improve the performance of traditional wastewater treatment facilities for quinoline removal. The effect of inoculum size on bioaugmentation was also investigated in this paper.展开更多
基金financial supports by the National Natural Science Foundation of China (Grant No. 21505156)the Fundamental Research Funds for the Central Universities (24720122043)the Scientific Research and Technological Development Projects of PetroChina Co., Ltd. (2013F-2101)
文摘Several strategies with different combination of inocula and packing material were investigated to obtain the optimal start-up time and elimination capacity (EC) in toluene biofiltration. The inocula contained the activated sludge and toluene degrading bacteria, and the packing material consisted of different mixing ratios of peat and wood chips. A final toluene load of 21.2 g/(m3·h) was attained step by step in four parallel biofilters. A shortest start-up time of 15 days and a highest EC of 17.0 g/(m3·h) were observed in the biofilter B-4, which was inoculated with a special microbial consortium consisting of three strains of toluene degrading bacteria and was packed with the mixture of peat and wood chips at a ratio of 80:20 (w/w). These results indicated that inoculating pre-acclimatized microbes dramatically shortened the start-up time, and such a composition of packing material could maintain an appropriate environment (with the bed porosity and water content equating to 0.45 and 1.96, respectively) for the growth of dominant toluene degrading bacteria in the biofilter.
文摘The application of arbuscular mycorrhizal (AM) inocula in severely degraded wetlands could ensure success in restoration.Mycorrhizal fungi play an important role in plant individual's survival and development in a low nutrient condition.Based on the importance that mycorrhizal fungi have to their host plants,mycorrhizal inocula have been produced and applied in terrestrial ecosystems in order to let the plants become mycorrhizal.However,mycorrhizal inocula have not been used in wetland restorations,despite increasing evidence that mycorrhizal fungi are commonly found in various wetland systems and have the ability to survive under anoxic conditions.Evidence also shows that mycorrhizal fungal inocula in the soil could have been destroyed in the degraded wetland or could be destroyed during traditional wetland restoration process.Therefore,AM inocula production is strongly recommended for wetland restoration.In this paper,I will argue that AM inocula production is required when introduced recovery is necessary,and aeroponic culture technique is a preferable method to produce AM inocula.Last,a renewed wetland restoration flow chart is summarized.
文摘To demonstrate the feasibility of using bioaugmentation to enhance biodegradation of quinoline, four strains capable of using quinoline as sole source of carbon, nitrogen and energy were isolated from different environmental samples by enrichment technique. Screening for quinoline degrader with the highest quinoline mineralizing rate was carried out in respirometer and one bacterium identified as \%Burkholderia pickettii W2\% was chosen as inoculum in bioaugmentation tests. Quinoline biodegradation experiment results showed that this bacterium degraded quinoline very quickly. 100, 200 and 500 mg/L quinoline can be transformed completely within 1, 2 and 7 hours respectively. A bioaugmentation procedure was proposed and laboratory experiments confirmed that bioaugmentation was an effective way to improve the performance of traditional wastewater treatment facilities for quinoline removal. The effect of inoculum size on bioaugmentation was also investigated in this paper.
