从小麦根际土壤分离得到枯草芽孢杆菌(Bacillus subtilis)B2-47拮抗菌株,室内测定其带菌培养液对小麦全蚀病菌(Gaeumannomyces graminis var tritici)9826菌株和9812菌株的抑制效果分别为61%和56%,盆栽试验中,其防治效果为63%和64%,高...从小麦根际土壤分离得到枯草芽孢杆菌(Bacillus subtilis)B2-47拮抗菌株,室内测定其带菌培养液对小麦全蚀病菌(Gaeumannomyces graminis var tritici)9826菌株和9812菌株的抑制效果分别为61%和56%,盆栽试验中,其防治效果为63%和64%,高于化学杀菌剂处理的56%和54%的效果.实验表明,该菌株可以造成病菌菌丝发生畸变和菌丝细胞壁瓦解.展开更多
In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with ...In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with a broad spectrum of antimicrobial effect.It is vital to inhibit the spread of pathogen in hospital.However,a large number of pathogens with the decreased antiseptic susceptibility have been isolated from clinical samples which showed an increased minimal inhibitory concentration(MIC)against those antiseptics.These resistant pathogens are the major causes for nosocomial crossinfections in hospital.The present study demonstrated the utility of Oxford plate assay system in determining the potential disinfectant resistance of bacteria.The microbiological assay is based on the inhibitory effect of tested disinfectants upon the strains of Staphylococcus aureus and Escherichia coli.Statistical analysis of the bioassay results indicated the linear correlation(r=-0.87-0.99,P<0.01)between the diameter of growth inhibition zone and the log dosage of the tested disinfectants.Moreover,comparison of inhibitory efficacy of benzalkonium bromide upon 29 S.aureus strains isolated from clinical samples by both Oxford plate method and broth dilution method showed that the diameter of growth inhibition zone has significantly negative correlation with the minimal inhibitory concentration(MIC)(r=-0.574,P<0.001).These results suggest that the Oxford plate is a simple and time-saving method in detecting potential clinical disinfectant resistance and its usefulness for routine surveillance of pathogenic resistance to disinfectants warrants further investigation.展开更多
在室内以甲霜灵为化学对照，80％乙醇为空白对照测试了菊花乙醇提取物对番茄晚疫病菌（Phytophthora in festans）菌丝生长和孢子囊萌发的抑制作用，以及光照、温度对菊花提取物抑菌效果的影响。结果表明：菊花的乙醇提取物抑制番茄晚...在室内以甲霜灵为化学对照，80％乙醇为空白对照测试了菊花乙醇提取物对番茄晚疫病菌（Phytophthora in festans）菌丝生长和孢子囊萌发的抑制作用，以及光照、温度对菊花提取物抑菌效果的影响。结果表明：菊花的乙醇提取物抑制番茄晚疫病菌菌丝生长的有效中浓度为稀释470倍，抑制孢子囊萌发的有效中浓度为稀释1055倍；光照强度在6000-80000 1lx范围内，温度在40-90℃范围内，菊花提取物的抑菌效果和化学药剂甲霜灵差异不显著。展开更多
文摘从小麦根际土壤分离得到枯草芽孢杆菌(Bacillus subtilis)B2-47拮抗菌株,室内测定其带菌培养液对小麦全蚀病菌(Gaeumannomyces graminis var tritici)9826菌株和9812菌株的抑制效果分别为61%和56%,盆栽试验中,其防治效果为63%和64%,高于化学杀菌剂处理的56%和54%的效果.实验表明,该菌株可以造成病菌菌丝发生畸变和菌丝细胞壁瓦解.
文摘In clinical practice,the important hygienic prevention of bacterial pathogen spread is disinfection of potentially contaminated area.Benzalkonium bromide and chlorhexidine acetate are commonly used disinfectants with a broad spectrum of antimicrobial effect.It is vital to inhibit the spread of pathogen in hospital.However,a large number of pathogens with the decreased antiseptic susceptibility have been isolated from clinical samples which showed an increased minimal inhibitory concentration(MIC)against those antiseptics.These resistant pathogens are the major causes for nosocomial crossinfections in hospital.The present study demonstrated the utility of Oxford plate assay system in determining the potential disinfectant resistance of bacteria.The microbiological assay is based on the inhibitory effect of tested disinfectants upon the strains of Staphylococcus aureus and Escherichia coli.Statistical analysis of the bioassay results indicated the linear correlation(r=-0.87-0.99,P<0.01)between the diameter of growth inhibition zone and the log dosage of the tested disinfectants.Moreover,comparison of inhibitory efficacy of benzalkonium bromide upon 29 S.aureus strains isolated from clinical samples by both Oxford plate method and broth dilution method showed that the diameter of growth inhibition zone has significantly negative correlation with the minimal inhibitory concentration(MIC)(r=-0.574,P<0.001).These results suggest that the Oxford plate is a simple and time-saving method in detecting potential clinical disinfectant resistance and its usefulness for routine surveillance of pathogenic resistance to disinfectants warrants further investigation.
文摘在室内以甲霜灵为化学对照，80％乙醇为空白对照测试了菊花乙醇提取物对番茄晚疫病菌（Phytophthora in festans）菌丝生长和孢子囊萌发的抑制作用，以及光照、温度对菊花提取物抑菌效果的影响。结果表明：菊花的乙醇提取物抑制番茄晚疫病菌菌丝生长的有效中浓度为稀释470倍，抑制孢子囊萌发的有效中浓度为稀释1055倍；光照强度在6000-80000 1lx范围内，温度在40-90℃范围内，菊花提取物的抑菌效果和化学药剂甲霜灵差异不显著。
