This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling （18% w/v raffinose, RS-C） were compared with spe...This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling （18% w/v raffinose, RS-C） were compared with spermatozoa vitrified using 0.25 M sucrose （SV） or 18% w/v raffinose （RV）. The motility, vitality, and DNA damage （TUNEL assay） of fresh control （FC） spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes （n = 267） were randomly assigned into three groups for insemination： RV （n = 102）, RS-C （n = 86）, and FC （n = 79）. The number and the proportion of two-cell embryos and blastocysts from each treatment were assessed. Sperm motility （P 〈 0.01） and vitality （P 〈 0.05） were significantly reduced after vitrification compared with slow-cooled spermatozoa. However, DNA fragmentation was significantly reduced in spermatozoa vitrified using sucrose （15 - 1.8% [SV] vs 26 - 2.8% [RV] and 27 - 1.2% [RS-C]; P 〈 0.01）. Although the number of two-cell embryos produced by RS-C, RV, and FC spermatozoa was not significantly different, the number of blastocysts produced from two-cell embryos using RV spermatozoa was significantly higher than FC spermatozoa （P = 0.0053）. This simple, small volume vitrification protocol and standard insemination method allows successful embryo production from small numbers of epididymal spermatozoa and may be applied clinically to circumvent the need for ICSI, which has the disadvantage of bypassing sperm selection.展开更多
目的:分析重组人生长激素(Recombinant Human Growth Hormone,rh-GH)预处理对剖宫产瘢痕憩室(PCSD)患者体外受精-胞浆内单精子显微注射技术(IVF/ICSI)治疗结局及基础促卵泡激素/基础黄体生成素(bFSH/bLH)比值的影响。方法:选取宜春市妇...目的:分析重组人生长激素(Recombinant Human Growth Hormone,rh-GH)预处理对剖宫产瘢痕憩室(PCSD)患者体外受精-胞浆内单精子显微注射技术(IVF/ICSI)治疗结局及基础促卵泡激素/基础黄体生成素(bFSH/bLH)比值的影响。方法:选取宜春市妇幼保健院2017年7月—2022年6月收治的120例PCSD进行IVF/ICSI治疗患者作为研究对象,按照随机数字表法将其分对照组和治疗组,每组60例。治疗组采用rh-GH进行预处理,对照组不进行预处理,比较两组治疗前后性激素水平,以及治疗后IVF/ICSI治疗结局、妊娠情况、胚胎质量。结果:治疗后,治疗组的优势卵泡数显著多于对照组(P<0.05);治疗后,治疗组的受精率、卵裂率、2PN卵裂率与对照组相比差异均无统计学意义(P>0.05),而治疗组的2PN受精率均显著高于对照组(P<0.05);治疗后,两组的bFSH、bLH、bFSH/bLH、E_(2)及P水平均显著降低(P<0.05),且治疗组的bFSH、bLH、bFSH/bLH、E_(2)及P水平均显著低于对照组(P<0.05);治疗后,治疗组的轻度卵巢过度刺激综合征(OHSS)发生率与对照组相比差异无统计学意义(P>0.05),而治疗组的优胚率(56.64%)、种植率(53.33%)及妊娠率(68.33%)均显著高于对照组的47.46%、18.33%、21.67%(P<0.05)。结论:采用rh-GH对PCSD患者IVF/ICSI治疗进行预处理获益明显。展开更多
目的研究男性乙型肝炎病毒(hepatitis B virus,HBV)感染对IVF/ICSI(in vitro fertilization or intracytoplasmic sperminjection)过程和周期结局的影响。方法 115对男方乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)阳性、女...目的研究男性乙型肝炎病毒(hepatitis B virus,HBV)感染对IVF/ICSI(in vitro fertilization or intracytoplasmic sperminjection)过程和周期结局的影响。方法 115对男方乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)阳性、女方阴性的夫妇作为实验组,对照组为夫妇双方HBsAg均阴性,根据HBV组女性年龄、月经第3天FSH值、体重指数和治疗方法(IVF或ICSI)按1∶2的比例进行配对,比较分析两组的FSH注射天数、Gn(gonadotropin)用量、HCG(human chorionic gonadotropin)日子宫内膜厚度和E2(estradiol)值、平均获卵数、成熟率、2PN(two pronucleus)受精率、卵裂率、优质胚胎率、临床妊娠率和种植率等参数。结果两组间各项指标均无统计学差异(P>0.05)。结论男性HBV感染不影响IVF/ICSI过程和周期结局。展开更多
文摘This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling （18% w/v raffinose, RS-C） were compared with spermatozoa vitrified using 0.25 M sucrose （SV） or 18% w/v raffinose （RV）. The motility, vitality, and DNA damage （TUNEL assay） of fresh control （FC） spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes （n = 267） were randomly assigned into three groups for insemination： RV （n = 102）, RS-C （n = 86）, and FC （n = 79）. The number and the proportion of two-cell embryos and blastocysts from each treatment were assessed. Sperm motility （P 〈 0.01） and vitality （P 〈 0.05） were significantly reduced after vitrification compared with slow-cooled spermatozoa. However, DNA fragmentation was significantly reduced in spermatozoa vitrified using sucrose （15 - 1.8% [SV] vs 26 - 2.8% [RV] and 27 - 1.2% [RS-C]; P 〈 0.01）. Although the number of two-cell embryos produced by RS-C, RV, and FC spermatozoa was not significantly different, the number of blastocysts produced from two-cell embryos using RV spermatozoa was significantly higher than FC spermatozoa （P = 0.0053）. This simple, small volume vitrification protocol and standard insemination method allows successful embryo production from small numbers of epididymal spermatozoa and may be applied clinically to circumvent the need for ICSI, which has the disadvantage of bypassing sperm selection.
文摘目的:分析重组人生长激素(Recombinant Human Growth Hormone,rh-GH)预处理对剖宫产瘢痕憩室(PCSD)患者体外受精-胞浆内单精子显微注射技术(IVF/ICSI)治疗结局及基础促卵泡激素/基础黄体生成素(bFSH/bLH)比值的影响。方法:选取宜春市妇幼保健院2017年7月—2022年6月收治的120例PCSD进行IVF/ICSI治疗患者作为研究对象,按照随机数字表法将其分对照组和治疗组,每组60例。治疗组采用rh-GH进行预处理,对照组不进行预处理,比较两组治疗前后性激素水平,以及治疗后IVF/ICSI治疗结局、妊娠情况、胚胎质量。结果:治疗后,治疗组的优势卵泡数显著多于对照组(P<0.05);治疗后,治疗组的受精率、卵裂率、2PN卵裂率与对照组相比差异均无统计学意义(P>0.05),而治疗组的2PN受精率均显著高于对照组(P<0.05);治疗后,两组的bFSH、bLH、bFSH/bLH、E_(2)及P水平均显著降低(P<0.05),且治疗组的bFSH、bLH、bFSH/bLH、E_(2)及P水平均显著低于对照组(P<0.05);治疗后,治疗组的轻度卵巢过度刺激综合征(OHSS)发生率与对照组相比差异无统计学意义(P>0.05),而治疗组的优胚率(56.64%)、种植率(53.33%)及妊娠率(68.33%)均显著高于对照组的47.46%、18.33%、21.67%(P<0.05)。结论:采用rh-GH对PCSD患者IVF/ICSI治疗进行预处理获益明显。
文摘目的研究男性乙型肝炎病毒(hepatitis B virus,HBV)感染对IVF/ICSI(in vitro fertilization or intracytoplasmic sperminjection)过程和周期结局的影响。方法 115对男方乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)阳性、女方阴性的夫妇作为实验组,对照组为夫妇双方HBsAg均阴性,根据HBV组女性年龄、月经第3天FSH值、体重指数和治疗方法(IVF或ICSI)按1∶2的比例进行配对,比较分析两组的FSH注射天数、Gn(gonadotropin)用量、HCG(human chorionic gonadotropin)日子宫内膜厚度和E2(estradiol)值、平均获卵数、成熟率、2PN(two pronucleus)受精率、卵裂率、优质胚胎率、临床妊娠率和种植率等参数。结果两组间各项指标均无统计学差异(P>0.05)。结论男性HBV感染不影响IVF/ICSI过程和周期结局。
