采用水提醇沉法结合冻融制备高纯度铁皮石斛多糖,并利用硫酸化、脱乙酰化及羧甲基化修饰方法对铁皮石斛多糖进行化学修饰,探讨不同方法修饰的铁皮石斛多糖对RAW264.7巨噬细胞免疫活性影响。结果表明,铁皮石斛纯多糖(purified polysaccha...采用水提醇沉法结合冻融制备高纯度铁皮石斛多糖,并利用硫酸化、脱乙酰化及羧甲基化修饰方法对铁皮石斛多糖进行化学修饰,探讨不同方法修饰的铁皮石斛多糖对RAW264.7巨噬细胞免疫活性影响。结果表明,铁皮石斛纯多糖(purified polysaccharides of Dendrobium officinale,DOP)的中性糖含量较高,而糖醛酸及蛋白质含量则较低,分别为87.37%、3.19%和0.51%,羧甲基化及硫酸化显著降低了铁皮石斛多糖的中性糖含量。红外分析结果显示硫酸化、脱乙酰化及羧甲基化修饰衍生物均已成功制备。高性能凝胶渗透色谱法测定铁皮石斛多糖分子质量为960 k D,硫酸化及羧甲基化修饰显著增加了其分子质量。体外实验研究发现,硫酸化和脱乙酰化修饰的铁皮石斛多糖能够增强RAW264.7巨噬细胞的免疫活性,而羧甲基化修饰则表现为显著的抑制作用,表明不是所有的官能团接枝改性都可以改善铁皮石斛多糖对免疫活性的影响。展开更多
Indeed,medicinal importance of honey has been documented in the world's oldest medical literatures,and since the ancient times,it has been known to possess antimicrobial property as well as wound-healing activity....Indeed,medicinal importance of honey has been documented in the world's oldest medical literatures,and since the ancient times,it has been known to possess antimicrobial property as well as wound-healing activity.The healing property of honey is due to the fact that it offers antibacterial activity,maintains a moist wound condition,and its high viscosity helps to provide a protective barrier to prevent infection.Its immunomodulatory property is relevant to wound repair too.The antimicrobial activity in most honeys is due to the enzymatic production of hydrogen peroxide.However,another kind of honey,called non-peroxide honey(viz.,manuka honey),displays significant antibacterial effects even when the hydrogen peroxide activity is blocked.Its mechanism may be related to the low pH level of honey and its high sugar content(high osmolality) that is enough to hinder the growth of microbes.The medical grade honeys have potent in vitro bactericidal activity against antibiotic-resistant bacteria causing several life-threatening infections to humans. But,there is a large variation in the antimicrobial activity of some natural honeys,which is due to spatial and temporal variation in sources of nectar.Thus,identification and characterization of the active principle(s) may provide valuable information on the quality and possible therapeutic potential of honeys(against several health disorders of humans),and hence we discussed the medicinal property of honeys with emphasis on their antibacterial activities.展开更多
目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色...目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色谱-十八角度激光光散射联用法(HPGPC-MALLS)测定PMT的绝对分子质量,采用PMP柱前衍生-高效液相色谱法(HPLC)测定单糖组成。采用二维核磁共振波谱(2D-NMR)对PMT的结构进行表征。采用四甲偶氮唑盐(MTT)法、中性红比色法和Griess法分别检测PMT对小鼠单核巨噬细胞RAW264.7的生长、吞噬活性和释放一氧化氮(NO)能力的影响。结果 PMT是一种α-1,4-葡聚糖,其绝对分子质量为3.96×105。PMT可显著促进RAW264.7细胞的增殖,促进细胞的吞噬能力,增加NO的释放量。结论何首乌来源的α-1,4-葡聚糖具有显著的免疫调节活性,具有药物开发潜力。展开更多
To recognize the potential medicinal value of the Dendrobium sonia, polysaccharide(DSP) was extracted, purified, and investigated for its immunomodulatory activity. In vitro, DSP was shown to enhance the viability(MTT...To recognize the potential medicinal value of the Dendrobium sonia, polysaccharide(DSP) was extracted, purified, and investigated for its immunomodulatory activity. In vitro, DSP was shown to enhance the viability(MTT assay) and phagocytosis of macrophages. In cyclophosphamide-induced immunosuppressed mice, DSP increased serum levels of TNF-α, IL-6 and IFN-γ(enzyme-linked immunosorbent assay, ELISA), and ameliorated the imbalance of the community of gut microbiota as detected by 16 S ribosomal RNA gene sequencing. These results suggest that DSP might be beneficial for patients under immunosuppressed conditions.展开更多
本研究采用热水浸提法(hot water extraction)、超声提取法(ultrasonic extraction)和超声波辅助热水提取法(ultrasonic-assisted hot water extraction)提取杏鲍菇多糖,将不同工艺提取多糖分别命名为H、U、U+H,并对其进行理化指标测定...本研究采用热水浸提法(hot water extraction)、超声提取法(ultrasonic extraction)和超声波辅助热水提取法(ultrasonic-assisted hot water extraction)提取杏鲍菇多糖,将不同工艺提取多糖分别命名为H、U、U+H,并对其进行理化指标测定、结构组成表征以及免疫活性分析,以探究不同提取工艺对杏鲍菇多糖结构特征及免疫活性的影响。结果显示,3种多糖均具有多糖类物质的特征吸收峰,U的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=8.60∶80.90∶7.41∶2.68∶0.57;H的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=9.36∶79.72∶8.18∶2.60∶0.42;U+H的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=6.75∶82.66∶7.14∶2.30∶1.12。U的分子质量分布为13152(分布比例83.61%)、281(分布比例3.02%)、53 kDa(分布比例13.37%);H的分子质量分布为10232(分布比例93.15%)、281(分布比例1.94%)、61 kDa(分布比例4.90%);U+H的分子质量分布为10471(分布比例98.59%)、60 kDa(分布比例1.40%)。细胞计数试剂盒(cell counting kit-8,CCK-8)检测结果表明,U在较低质量浓度(5、10μg/mL)下有轻微毒性作用,可能原因为大分子质量多糖降解,其余质量浓度U及所有质量浓度的H、U+H对巨噬细胞无明显毒性作用。中性红试剂盒测定结果表明3种多糖对巨噬细胞的吞噬活性均具有显著的增强作用(P<0.05),且3种多糖处理细胞的吞噬活性差异明显。其中,U组分最高吞噬率可达到193.45%,H和U+H组分最高吞噬率分别达到163.64%、187.62%。本研究可为定向制备具有特定功能活性的杏鲍菇多糖提供理论参考。展开更多
文摘采用水提醇沉法结合冻融制备高纯度铁皮石斛多糖,并利用硫酸化、脱乙酰化及羧甲基化修饰方法对铁皮石斛多糖进行化学修饰,探讨不同方法修饰的铁皮石斛多糖对RAW264.7巨噬细胞免疫活性影响。结果表明,铁皮石斛纯多糖(purified polysaccharides of Dendrobium officinale,DOP)的中性糖含量较高,而糖醛酸及蛋白质含量则较低,分别为87.37%、3.19%和0.51%,羧甲基化及硫酸化显著降低了铁皮石斛多糖的中性糖含量。红外分析结果显示硫酸化、脱乙酰化及羧甲基化修饰衍生物均已成功制备。高性能凝胶渗透色谱法测定铁皮石斛多糖分子质量为960 k D,硫酸化及羧甲基化修饰显著增加了其分子质量。体外实验研究发现,硫酸化和脱乙酰化修饰的铁皮石斛多糖能够增强RAW264.7巨噬细胞的免疫活性,而羧甲基化修饰则表现为显著的抑制作用,表明不是所有的官能团接枝改性都可以改善铁皮石斛多糖对免疫活性的影响。
文摘Indeed,medicinal importance of honey has been documented in the world's oldest medical literatures,and since the ancient times,it has been known to possess antimicrobial property as well as wound-healing activity.The healing property of honey is due to the fact that it offers antibacterial activity,maintains a moist wound condition,and its high viscosity helps to provide a protective barrier to prevent infection.Its immunomodulatory property is relevant to wound repair too.The antimicrobial activity in most honeys is due to the enzymatic production of hydrogen peroxide.However,another kind of honey,called non-peroxide honey(viz.,manuka honey),displays significant antibacterial effects even when the hydrogen peroxide activity is blocked.Its mechanism may be related to the low pH level of honey and its high sugar content(high osmolality) that is enough to hinder the growth of microbes.The medical grade honeys have potent in vitro bactericidal activity against antibiotic-resistant bacteria causing several life-threatening infections to humans. But,there is a large variation in the antimicrobial activity of some natural honeys,which is due to spatial and temporal variation in sources of nectar.Thus,identification and characterization of the active principle(s) may provide valuable information on the quality and possible therapeutic potential of honeys(against several health disorders of humans),and hence we discussed the medicinal property of honeys with emphasis on their antibacterial activities.
文摘目的从何首乌Polygonum multiflorum中提取分离多糖,对其结构进行表征,并评价其免疫调节活性。方法采用水提、醇沉法从何首乌中提取粗多糖,经Q-Sepharose Fast Flow强阴离子交换色谱柱分离纯化得到何首乌多糖(PMT)。利用高效凝胶渗透色谱-十八角度激光光散射联用法(HPGPC-MALLS)测定PMT的绝对分子质量,采用PMP柱前衍生-高效液相色谱法(HPLC)测定单糖组成。采用二维核磁共振波谱(2D-NMR)对PMT的结构进行表征。采用四甲偶氮唑盐(MTT)法、中性红比色法和Griess法分别检测PMT对小鼠单核巨噬细胞RAW264.7的生长、吞噬活性和释放一氧化氮(NO)能力的影响。结果 PMT是一种α-1,4-葡聚糖,其绝对分子质量为3.96×105。PMT可显著促进RAW264.7细胞的增殖,促进细胞的吞噬能力,增加NO的释放量。结论何首乌来源的α-1,4-葡聚糖具有显著的免疫调节活性,具有药物开发潜力。
基金supported by the Nanjing Science and Technology Commission(No.201722045)
文摘To recognize the potential medicinal value of the Dendrobium sonia, polysaccharide(DSP) was extracted, purified, and investigated for its immunomodulatory activity. In vitro, DSP was shown to enhance the viability(MTT assay) and phagocytosis of macrophages. In cyclophosphamide-induced immunosuppressed mice, DSP increased serum levels of TNF-α, IL-6 and IFN-γ(enzyme-linked immunosorbent assay, ELISA), and ameliorated the imbalance of the community of gut microbiota as detected by 16 S ribosomal RNA gene sequencing. These results suggest that DSP might be beneficial for patients under immunosuppressed conditions.
文摘本研究采用热水浸提法(hot water extraction)、超声提取法(ultrasonic extraction)和超声波辅助热水提取法(ultrasonic-assisted hot water extraction)提取杏鲍菇多糖,将不同工艺提取多糖分别命名为H、U、U+H,并对其进行理化指标测定、结构组成表征以及免疫活性分析,以探究不同提取工艺对杏鲍菇多糖结构特征及免疫活性的影响。结果显示,3种多糖均具有多糖类物质的特征吸收峰,U的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=8.60∶80.90∶7.41∶2.68∶0.57;H的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=9.36∶79.72∶8.18∶2.60∶0.42;U+H的单糖组成及其物质的量比为n(甘露糖)∶n(葡萄糖)∶n(半乳糖)∶n(木糖)∶n(岩藻糖)=6.75∶82.66∶7.14∶2.30∶1.12。U的分子质量分布为13152(分布比例83.61%)、281(分布比例3.02%)、53 kDa(分布比例13.37%);H的分子质量分布为10232(分布比例93.15%)、281(分布比例1.94%)、61 kDa(分布比例4.90%);U+H的分子质量分布为10471(分布比例98.59%)、60 kDa(分布比例1.40%)。细胞计数试剂盒(cell counting kit-8,CCK-8)检测结果表明,U在较低质量浓度(5、10μg/mL)下有轻微毒性作用,可能原因为大分子质量多糖降解,其余质量浓度U及所有质量浓度的H、U+H对巨噬细胞无明显毒性作用。中性红试剂盒测定结果表明3种多糖对巨噬细胞的吞噬活性均具有显著的增强作用(P<0.05),且3种多糖处理细胞的吞噬活性差异明显。其中,U组分最高吞噬率可达到193.45%,H和U+H组分最高吞噬率分别达到163.64%、187.62%。本研究可为定向制备具有特定功能活性的杏鲍菇多糖提供理论参考。
