Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivatio...Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfedng RNA （siRNA）-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like re- ceptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.展开更多
目的分析不同程度重度脑血管病患者血清C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平与CD_4^+细胞、CD_4^+/CD8+T细胞比例及CD_4^+CD_(25)^+T细胞百分比变化,分析CD_4^+细胞、CD_4^+CD_(25)^+T细胞与患者昏迷程度评分间关系。方法选择2...目的分析不同程度重度脑血管病患者血清C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平与CD_4^+细胞、CD_4^+/CD8+T细胞比例及CD_4^+CD_(25)^+T细胞百分比变化,分析CD_4^+细胞、CD_4^+CD_(25)^+T细胞与患者昏迷程度评分间关系。方法选择2013年3月至2014年10月诊断为重症脑血管病患者70例,依据入院24 h内格拉拉斯哥昏迷评分(Glasgow Coma Scale,GCS)将患者分为重症组30例(GCS,7~9)与特重组40例(GCS,3~6)。e Lisa检测患者血清超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)水平,流式细胞术检测外周血CD_4^+细胞、CD_4^+/CD8+T细胞比例及CD_4^+CD_(25)^+T细胞百分比变化,Pearson相关分析CD_4^+细胞、CD_4^+CD_(25)^+T细胞比例与GCS评分之间相关性。结果重症组与特重症组比较,患者血清CRP水平[(22.85±4.81)mg/L比(32.80±5.26)mg/L,t=2.901],TNF-α水平[(26.84±5.10)ng/L比(44.18±4.39)ng/L,t=3.166]明显降低,2组比较差异有统计学意义(P<0.05)。外周血CD_4^+细胞百分比[(31.65±6.17)%比(26.90±5.88)%,t=3.082],CD_4^+/CD8+T细胞比例[(1.89±0.31)比(1.23±0.43),t=3.048]升高,CD_4^+CD_(25)^+T细胞百分比[(25.12±3.28)%比(34.17±4.26)%,t=3.543]下降,2组比较差异有统计学意义(P<0.05)。CD_4^+细胞百分比与GCS评分呈负相关(r=-5.651,P<0.05),CD_4^+/CD8+T细胞比例与GCS呈直线正相关(r=4.872,P<0.05)。结论机体免疫炎性反应在急性重症脑血管病患者起病中占有重要地位,病情程度越严重,机体免疫抑制以及炎性反应越明显。表现为血清超敏C反应蛋白、肿瘤坏死因子-α水平升高以及CD_4^+细胞、CD_4^+/CD8+T细胞比例下降、CD_4^+CD_(25)^+T细胞百分比水平上升。展开更多
基金supported by the National Natural Science Foundation of China,No.30970995the Postgraduate Science Research Innovation Project of Higher Learning University of Jiangsu Province in China,No.CXLX11_0735
文摘Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ische- mia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxy- gen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfedng RNA （siRNA）-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like re- ceptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.
文摘目的分析不同程度重度脑血管病患者血清C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平与CD_4^+细胞、CD_4^+/CD8+T细胞比例及CD_4^+CD_(25)^+T细胞百分比变化,分析CD_4^+细胞、CD_4^+CD_(25)^+T细胞与患者昏迷程度评分间关系。方法选择2013年3月至2014年10月诊断为重症脑血管病患者70例,依据入院24 h内格拉拉斯哥昏迷评分(Glasgow Coma Scale,GCS)将患者分为重症组30例(GCS,7~9)与特重组40例(GCS,3~6)。e Lisa检测患者血清超敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)水平,流式细胞术检测外周血CD_4^+细胞、CD_4^+/CD8+T细胞比例及CD_4^+CD_(25)^+T细胞百分比变化,Pearson相关分析CD_4^+细胞、CD_4^+CD_(25)^+T细胞比例与GCS评分之间相关性。结果重症组与特重症组比较,患者血清CRP水平[(22.85±4.81)mg/L比(32.80±5.26)mg/L,t=2.901],TNF-α水平[(26.84±5.10)ng/L比(44.18±4.39)ng/L,t=3.166]明显降低,2组比较差异有统计学意义(P<0.05)。外周血CD_4^+细胞百分比[(31.65±6.17)%比(26.90±5.88)%,t=3.082],CD_4^+/CD8+T细胞比例[(1.89±0.31)比(1.23±0.43),t=3.048]升高,CD_4^+CD_(25)^+T细胞百分比[(25.12±3.28)%比(34.17±4.26)%,t=3.543]下降,2组比较差异有统计学意义(P<0.05)。CD_4^+细胞百分比与GCS评分呈负相关(r=-5.651,P<0.05),CD_4^+/CD8+T细胞比例与GCS呈直线正相关(r=4.872,P<0.05)。结论机体免疫炎性反应在急性重症脑血管病患者起病中占有重要地位,病情程度越严重,机体免疫抑制以及炎性反应越明显。表现为血清超敏C反应蛋白、肿瘤坏死因子-α水平升高以及CD_4^+细胞、CD_4^+/CD8+T细胞比例下降、CD_4^+CD_(25)^+T细胞百分比水平上升。
