Background The usefulness of in-phase/opposed-phase imaging and diffusion weighted imaging （DWI） in differentiating benign and neoplastic vertebral fractures has been described. In this study, we aimed to evaluate t...Background The usefulness of in-phase/opposed-phase imaging and diffusion weighted imaging （DWI） in differentiating benign and neoplastic vertebral fractures has been described. In this study, we aimed to evaluate the influence of the severity of vertebral damage on the diagnostic performance of these two technologies. Methods Totally 59 patients with 68 acute benign vertebral fractures and 43 patients with 79 vertebral metastases were included in this study. The MR protocol included DWls and sagittal in-phase/opposed-phase gradient recalled sequence. The severity of vertebral damage was expressed by lesion ratio （LR, the ratio of lesion area to vertebral area on the slices of largest abnormal signal area in the Tl-weighted sequence）. Quantitative （signal intensity ratio （SIR） defined as signal intensity （SI） on opposed-phase gradient recalled echo （GRE） images divided by SI on in-phase; apparent diffusion coefficient （ADC） value derived from DWl analysis was performed, the relationships between LR and the measurements of these two technologies were analyzed using linear regression. The covariate-specific receiver operating characteristic （ROC） curves were also fitted to evaluate the influence of LR on the diagnostic performance of ADC and SIR. Results The difference in both SIR and ADC for vertebral metastasis and acute benign vertebral fractures was significant （P 〈0.001）. A positive correlation between the LR and the SIR was found in benign fractures （P 〈0.05）. The severity of vertebral damage had a significant influence on the AUC （area under ROC curve） for SIR （P 〈0.05） but ADC （P 〉0.05）. More severe cases were associated with increased AUC for SIR. Conclusions LR is capable of affecting the diagnostic performances of chemical shift imaging. Thus, when applying these tests to make diagnoses on vertebral fractures, the severity of the vertebral damage should be taken into account. The covariate-specific ROC model is recommended because it substantially展开更多
Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atheroscleros...Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signature of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD.展开更多
Principles of terahertz （THz） interferometric synthetic aperture imaging with heterodyne and optical tech- niques are presented. A THz interferometric experiment based on optical up-conversion is set up. The receive...Principles of terahertz （THz） interferometric synthetic aperture imaging with heterodyne and optical tech- niques are presented. A THz interferometric experiment based on optical up-conversion is set up. The received THz signal is modulated into an optical carrier and transmitted in a fiber. To simulate phase differences between two THz receivers, the output of receiver is divided and a phase shifter is placed before electro-optical modulation （EOM）. Interferometric spectra of these modulated optical signals are examined at different phase shifts. Otherwise, carrier suppression and phase error calibration are discussed for THz interferometric synthetic aperture imaging.展开更多
A new schiff base of phenothiazine derivative was designed for ratiometric sensing of Cd2＋ selectively. Upon the addition of Cd2＋ to the solution of phenothiazine derivative, the fluorescence intensity of it was enh...A new schiff base of phenothiazine derivative was designed for ratiometric sensing of Cd2＋ selectively. Upon the addition of Cd2＋ to the solution of phenothiazine derivative, the fluorescence intensity of it was enhanced in a linear fashion and the maximum fluorescence peak exhibited a blue shift from 575 nm to 525 nm. This ratiometric fluorescent sensor displays a very high sensitivity（detection limits were 0.34 and 1.0μmol/L of Cd2＋ using the visual fluorescence changes and UV-Vis changes, respectively）, a rapid response time（〈10 s） and high selectivity for Cd2＋ over other transition metal ions. Moreover, the living cells image experiments also demonstrate the value of the sen- sor in fluorescent visualization of Cd2＋ in the environmental and biological systems.展开更多
文摘Background The usefulness of in-phase/opposed-phase imaging and diffusion weighted imaging （DWI） in differentiating benign and neoplastic vertebral fractures has been described. In this study, we aimed to evaluate the influence of the severity of vertebral damage on the diagnostic performance of these two technologies. Methods Totally 59 patients with 68 acute benign vertebral fractures and 43 patients with 79 vertebral metastases were included in this study. The MR protocol included DWls and sagittal in-phase/opposed-phase gradient recalled sequence. The severity of vertebral damage was expressed by lesion ratio （LR, the ratio of lesion area to vertebral area on the slices of largest abnormal signal area in the Tl-weighted sequence）. Quantitative （signal intensity ratio （SIR） defined as signal intensity （SI） on opposed-phase gradient recalled echo （GRE） images divided by SI on in-phase; apparent diffusion coefficient （ADC） value derived from DWl analysis was performed, the relationships between LR and the measurements of these two technologies were analyzed using linear regression. The covariate-specific receiver operating characteristic （ROC） curves were also fitted to evaluate the influence of LR on the diagnostic performance of ADC and SIR. Results The difference in both SIR and ADC for vertebral metastasis and acute benign vertebral fractures was significant （P 〈0.001）. A positive correlation between the LR and the SIR was found in benign fractures （P 〈0.05）. The severity of vertebral damage had a significant influence on the AUC （area under ROC curve） for SIR （P 〈0.05） but ADC （P 〉0.05）. More severe cases were associated with increased AUC for SIR. Conclusions LR is capable of affecting the diagnostic performances of chemical shift imaging. Thus, when applying these tests to make diagnoses on vertebral fractures, the severity of the vertebral damage should be taken into account. The covariate-specific ROC model is recommended because it substantially
基金National Natural Science Foundation of China(Nos.22107029,22377135,22305178,92253306 and 82130099)the Taishan Scholars Program(No.tsqn202312305)+7 种基金the Young Elite Scientists Sponsorship Program by Chinese Chemical Societythe Fundamental Research Projects of Science&Technology Innovation and development Plan in Yantai City(No.2023JCYJ059)the Shandong Laboratory Program(No.SYS202205)the Fundamental Research Funds for the Central Universities(No.222201717003)the Programme of Introducing Talents of Discipline to Universities(No.B16017)the Open Funding Project of the State Key Laboratory of Bioreactor Engineeringthe State Key Laboratory of Drug Research(No.SKLDR-2023-KF-10)the Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer(Naval Medical University)(No.2023-MEKLLCMS/ZD-00*)。
文摘Non-alcoholic fatty liver disease(NAFLD)is prevalent worldwide as a chronic liver disease that not only gives rise to hepatic complications,but leads to other chronic diseases such as type 2 diabetes and atherosclerosis.The aberrant accumulation of lipid droplets(LDs)in hepatocytes is a prominent signature of NAFLD.However,conventional techniques lack the capability to effectively monitor the dynamic changes in LD levels during NAFLD with living organisms.Hence,it is imperative to develop LD-specific long-wavelength fluorescent probes with high imaging contrast for the in-situ diagnosis of NAFLD.In this study,we synthesized a new LD-selective long-wavelength fluorescent probe,denoted as LD-1,based on the twisted intramolecular charge transfer(TICT)mechanism.The probe exhibits a large Stokes shift and intensive fluorescence emission in nonpolar and viscous solutions.By self-assembling LD-1 with bovine serum albumin(BSA),a biocompatible,long-wavelength fluorescent probe hybrid,LD-1@BSA,was formed,allowing for LDs to be selectively imaged in hepatocytes.Moreover,LD-1@BSA successfully discriminates NAFLD cells before and after drug treatment,and achieves non-invasive and real-time monitoring of LD accumulation in a mouse model of NAFLD.
基金supported by the National High-Tech Research and Development Program(No. 2007AA12Z114)the Innovation Foundation of BUAA for PhD Graduates.
文摘Principles of terahertz （THz） interferometric synthetic aperture imaging with heterodyne and optical tech- niques are presented. A THz interferometric experiment based on optical up-conversion is set up. The received THz signal is modulated into an optical carrier and transmitted in a fiber. To simulate phase differences between two THz receivers, the output of receiver is divided and a phase shifter is placed before electro-optical modulation （EOM）. Interferometric spectra of these modulated optical signals are examined at different phase shifts. Otherwise, carrier suppression and phase error calibration are discussed for THz interferometric synthetic aperture imaging.
文摘A new schiff base of phenothiazine derivative was designed for ratiometric sensing of Cd2＋ selectively. Upon the addition of Cd2＋ to the solution of phenothiazine derivative, the fluorescence intensity of it was enhanced in a linear fashion and the maximum fluorescence peak exhibited a blue shift from 575 nm to 525 nm. This ratiometric fluorescent sensor displays a very high sensitivity（detection limits were 0.34 and 1.0μmol/L of Cd2＋ using the visual fluorescence changes and UV-Vis changes, respectively）, a rapid response time（〈10 s） and high selectivity for Cd2＋ over other transition metal ions. Moreover, the living cells image experiments also demonstrate the value of the sen- sor in fluorescent visualization of Cd2＋ in the environmental and biological systems.
