Tests show the monoclonal anti CD4 antibody (mAb) MT310 recognizes the gp120 binding site on CD4 as part of its mechanism for strongly inhibiting human immunodeficiency virus type 1 (HIV 1) infection of CD4 + T ...Tests show the monoclonal anti CD4 antibody (mAb) MT310 recognizes the gp120 binding site on CD4 as part of its mechanism for strongly inhibiting human immunodeficiency virus type 1 (HIV 1) infection of CD4 + T cells. In competition tests, mAb MT310 and mAb Leu3a (an anti CD4 mAb recognizing the gp120 binding site) all inhibited gp120 binding to CD4 + T lymphocytes, while mAb MT405 did not. This result suggests that MT310, like Leu3a, recognizes the gp120 binding site on CD4. To further confirm whether MT310 recognizes the gp120 binding site on CD4, we prepared rabbit anti idiotypic antisera (Ab2) against MT310 (Ab1). The anti idiotypic antisera against MT310 inhibited binding of MT310 and Leu3a to human CD4 + T lymphocytes, but did not block binding of MT151 with the second domain of CD4, while rabbit anti idiotypic antisera to MT151 could block binding of itself to these cells, but could not inhibit the binding of MT310 and Leu3a, further indicating that MT310 recognized the gp120 binding site on CD4. 展开更多
Objective To generate and characterize anti-idiotypic monoclonal antibody (Ab2) that bears the internal image of nasopharyngeal carcinoma (NPC) associated antigen. Methods Using NPC monoclonal antibody (Ab1) as immu...Objective To generate and characterize anti-idiotypic monoclonal antibody (Ab2) that bears the internal image of nasopharyngeal carcinoma (NPC) associated antigen. Methods Using NPC monoclonal antibody (Ab1) as immunogen, hybridoma cells were obtained by fusion of SP2/0 myeloma cells with immunized murine spleen cells. Positive clones were screened by Sandwich ELISA and a binding inhibition test. To determine whether Ab2 possess the internal image of the original antigen or not, mice were immunized with Ab2. ELISA and the competitive inhibition assay tested anti-anti-idiotypic antibodies (Ab3) in anti-sera. Cell-mediated immunity to tumors induced by Ab2 was investigated by a delayed-type hypersensitivity response and the mouse T-cell proliferation assay. Results Anti-idiotypic monoclonal antibodies against the monoclonal anti-NPC antibodies FC2 and HNL5 were generated that recognize NPC associated antigens. These Ab2, which were designated 2H4 and 5D3, could inhibit the binding of FC2 or HNL5 to NPC cell lines. Anti-sera from the immunized mice, which contained Ab3, could compete with FC2 or HNL5 for binding with NPC cell by a competitive inhibition assay. Mice immunized with 2H4 or 5D3 coupled with keyhole limpet hemocyanin (KLH), showed a positive and specific delayed-type hypersensitivity (DTH) reaction after stimulation by NPC cells. The mouse T cell proliferative assay indicated that there was a significantly higher proliferative response of the splenocytes in the experimental groups than that in control groups. Conclusions Anti-idiotypic antibodies 2H4 and 5D3 are Ab2 beta bearing the internal image of the epitope of NPC associated antigen. Either 2H4 or 5D3 expressing three-dimensional shapes that resemble the structure of natural antigens could induce humoral and cellular immune response.展开更多
Objective To observe the effects of anti fecundity and anti embryonation immuntiy of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum on female adult worm Methods The active immunization ...Objective To observe the effects of anti fecundity and anti embryonation immuntiy of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum on female adult worm Methods The active immunization of C57BL/6 mice was conducted by means of three intraperitoneal injections of NP30 The control group was injected with SP2/0 ascites intraperitoneally Results On the twenty seventh day after challenge infection, the number of eggs in the liver tissue and in uterus of the group immunized with NP30 decreased by 30 91% and by 38 55%, respectively On the thirty ninth day after the challenge infection, the number of mature eggs in the liver tissue of the group immunized with NP30 decreased by 66 63% and the number of dead eggs increased by 60 66% Conclusions NP30, with which mice were actively immunized, possesses double effects of anti fecundity and anti embryonation immunity on female adult worm of Schistosoma japonicum , therefore it can be used as a promising candidate of anti pathologic vaccine molecule against Schistosomiasis japonica展开更多
To investigate the effect of active immunotherapy with anti idiotypic vaccine i n patients with nasopharyngeal carcinoma (NPC) Methods Anti idiotypic antibodies (2H4/5D3) bearing the internal image of the NPC ant...To investigate the effect of active immunotherapy with anti idiotypic vaccine i n patients with nasopharyngeal carcinoma (NPC) Methods Anti idiotypic antibodies (2H4/5D3) bearing the internal image of the NPC antig en were used in active immunotherapy in NPC patients receiving radiotherapy Antibodies and cytokine levels in patient sera were determined using ELISA befo re and after active immunotherapy IL 2 mRNA expression in the peripheral bloo d mononuclear cells (PBMC) was measured by in situ hybridization Results Nineteen patients with NPC at stage Ⅳ were treated with alum precipitated 2H4 or 5D3 Neither hypersensitivity nor adverse side effects were observed The l evels of anti anti idiotypic antibodies (Ab3) and anti NPC antibodies (Ab1’) were increased Human anti mouse antibodies (HAMA) were seen in 19 patients of the experimental group; the levels of Ab1’ did not increse in the control group Serum IL 2, IFN γ and TNF α levels were increased in most patients in th e experimental group, while no differences were observed in Ab1’ and cytokine le vels between pre and post therapy in the control group In addition, IL 2 m RNA expression in PBMCs from NPC patients was closely related to serum IL 2 ( r =+0 8829) levels by in situ hybridization Conclusions Anti idiotype vaccine is safe for clinical active immunotherapy Anti idiotyp ic vaccine might be able to enhance humoral and/or cellular immunity in NPC pati ents receiving radiotherapy展开更多
基金Munich University in Germany and theNational Key Basic Research Specific Funds of China(No.G19990 75 6 0 7)
文摘Tests show the monoclonal anti CD4 antibody (mAb) MT310 recognizes the gp120 binding site on CD4 as part of its mechanism for strongly inhibiting human immunodeficiency virus type 1 (HIV 1) infection of CD4 + T cells. In competition tests, mAb MT310 and mAb Leu3a (an anti CD4 mAb recognizing the gp120 binding site) all inhibited gp120 binding to CD4 + T lymphocytes, while mAb MT405 did not. This result suggests that MT310, like Leu3a, recognizes the gp120 binding site on CD4. To further confirm whether MT310 recognizes the gp120 binding site on CD4, we prepared rabbit anti idiotypic antisera (Ab2) against MT310 (Ab1). The anti idiotypic antisera against MT310 inhibited binding of MT310 and Leu3a to human CD4 + T lymphocytes, but did not block binding of MT151 with the second domain of CD4, while rabbit anti idiotypic antisera to MT151 could block binding of itself to these cells, but could not inhibit the binding of MT310 and Leu3a, further indicating that MT310 recognized the gp120 binding site on CD4.
基金ThisinvestigationwassupportedbygrantsfromtheNationalNaturalScienceFoundationofChina (No 392 70 716 )ChinaMedicalBoardinNewYork (No 90 5 2 9project 7)
文摘Objective To generate and characterize anti-idiotypic monoclonal antibody (Ab2) that bears the internal image of nasopharyngeal carcinoma (NPC) associated antigen. Methods Using NPC monoclonal antibody (Ab1) as immunogen, hybridoma cells were obtained by fusion of SP2/0 myeloma cells with immunized murine spleen cells. Positive clones were screened by Sandwich ELISA and a binding inhibition test. To determine whether Ab2 possess the internal image of the original antigen or not, mice were immunized with Ab2. ELISA and the competitive inhibition assay tested anti-anti-idiotypic antibodies (Ab3) in anti-sera. Cell-mediated immunity to tumors induced by Ab2 was investigated by a delayed-type hypersensitivity response and the mouse T-cell proliferation assay. Results Anti-idiotypic monoclonal antibodies against the monoclonal anti-NPC antibodies FC2 and HNL5 were generated that recognize NPC associated antigens. These Ab2, which were designated 2H4 and 5D3, could inhibit the binding of FC2 or HNL5 to NPC cell lines. Anti-sera from the immunized mice, which contained Ab3, could compete with FC2 or HNL5 for binding with NPC cell by a competitive inhibition assay. Mice immunized with 2H4 or 5D3 coupled with keyhole limpet hemocyanin (KLH), showed a positive and specific delayed-type hypersensitivity (DTH) reaction after stimulation by NPC cells. The mouse T cell proliferative assay indicated that there was a significantly higher proliferative response of the splenocytes in the experimental groups than that in control groups. Conclusions Anti-idiotypic antibodies 2H4 and 5D3 are Ab2 beta bearing the internal image of the epitope of NPC associated antigen. Either 2H4 or 5D3 expressing three-dimensional shapes that resemble the structure of natural antigens could induce humoral and cellular immune response.
文摘Objective To observe the effects of anti fecundity and anti embryonation immuntiy of anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum on female adult worm Methods The active immunization of C57BL/6 mice was conducted by means of three intraperitoneal injections of NP30 The control group was injected with SP2/0 ascites intraperitoneally Results On the twenty seventh day after challenge infection, the number of eggs in the liver tissue and in uterus of the group immunized with NP30 decreased by 30 91% and by 38 55%, respectively On the thirty ninth day after the challenge infection, the number of mature eggs in the liver tissue of the group immunized with NP30 decreased by 66 63% and the number of dead eggs increased by 60 66% Conclusions NP30, with which mice were actively immunized, possesses double effects of anti fecundity and anti embryonation immunity on female adult worm of Schistosoma japonicum , therefore it can be used as a promising candidate of anti pathologic vaccine molecule against Schistosomiasis japonica
文摘To investigate the effect of active immunotherapy with anti idiotypic vaccine i n patients with nasopharyngeal carcinoma (NPC) Methods Anti idiotypic antibodies (2H4/5D3) bearing the internal image of the NPC antig en were used in active immunotherapy in NPC patients receiving radiotherapy Antibodies and cytokine levels in patient sera were determined using ELISA befo re and after active immunotherapy IL 2 mRNA expression in the peripheral bloo d mononuclear cells (PBMC) was measured by in situ hybridization Results Nineteen patients with NPC at stage Ⅳ were treated with alum precipitated 2H4 or 5D3 Neither hypersensitivity nor adverse side effects were observed The l evels of anti anti idiotypic antibodies (Ab3) and anti NPC antibodies (Ab1’) were increased Human anti mouse antibodies (HAMA) were seen in 19 patients of the experimental group; the levels of Ab1’ did not increse in the control group Serum IL 2, IFN γ and TNF α levels were increased in most patients in th e experimental group, while no differences were observed in Ab1’ and cytokine le vels between pre and post therapy in the control group In addition, IL 2 m RNA expression in PBMCs from NPC patients was closely related to serum IL 2 ( r =+0 8829) levels by in situ hybridization Conclusions Anti idiotype vaccine is safe for clinical active immunotherapy Anti idiotyp ic vaccine might be able to enhance humoral and/or cellular immunity in NPC pati ents receiving radiotherapy
