Objective: To elucidate the role of S-100B and neuron specific enolase (NSE) in predicting the outcomes of patients with severe head injury. Methods: Forty patients with severe head injury were included in this study....Objective: To elucidate the role of S-100B and neuron specific enolase (NSE) in predicting the outcomes of patients with severe head injury. Methods: Forty patients with severe head injury were included in this study. The serum concentrations of S-100B and NSE were measured within 12 hours after head injury to investigate the correlation between serum levels of S-100B and NSE and outcome. Validity of both S-100B and NSE in outcome prediction was assessed with Receiver Operator Characteristic (ROC) curve. Results: The serum concentrations of S-100B and NSE of both groups, with favorable or unfavorable outcomes, were significantly higher than those of the normal group. The serum concentrations within 12 hours after head injury were closely correlated with the prognosis. Furthermore, according to the ROC curves of S-100B and NSE, S-100B was found better in predicting outcomes than NSE. Conclusions: S-100B and NSE may play important roles in outcome prediction after severe head injury. Moreover, S-100B is clearly superior to NSE in terms of predictive value and appears to be a more promising serum marker in outcome prediction after severe head injury.展开更多
Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-ste...Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-steady state assumption.Effects of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C.展开更多
A new approach to optically active β-alkyl-α-methylene-γ-butyrolactone derivatives was reported from the Rhodococcus sp. AJ270-catalyzed hydrolysis of appropriate nitriles. The inversion of enantioselectivity of th...A new approach to optically active β-alkyl-α-methylene-γ-butyrolactone derivatives was reported from the Rhodococcus sp. AJ270-catalyzed hydrolysis of appropriate nitriles. The inversion of enantioselectivity of the amidase has been observed when a methyl protection was introduced into the hydroxy group of the parent substrate.展开更多
Catalyzed by Rhodococcus erythropolis AJ270, a nitrile hydratase and amidase containing microbial whole-cell catalyst, at 10 ℃ and with the use of methanol as a co-solvent, nitrile and amide biotransformations produc...Catalyzed by Rhodococcus erythropolis AJ270, a nitrile hydratase and amidase containing microbial whole-cell catalyst, at 10 ℃ and with the use of methanol as a co-solvent, nitrile and amide biotransformations produce 2S-1,4-benzodioxane-2-carboxamide and 2R-1,4-benzodioxane-2-carboxylic acid in high yields with excellent enantioselectivity.展开更多
Inducing expression and the reaction characteristic of nitrile hydratase （NHase） from Rhodococcus sp. SHZ-1 were investigated. The results showed that the expression of NHase was greatly enhanced with the cooperatio...Inducing expression and the reaction characteristic of nitrile hydratase （NHase） from Rhodococcus sp. SHZ-1 were investigated. The results showed that the expression of NHase was greatly enhanced with the cooperation of acrylonitrile and ammonium chloride as inducer in the medium and the specific activity of NHase was increased of 44%. Then the temperature, pH, concentration of acrylonitrile and acrylamide were evaluated, which affected the activity and reaction characteristic of NHase. It was found that the temperature and concentration of acrylarnide were the most important factors for the catalyzation of NHase. The optimal catalysis temperature of NHase from Rhodococcus sp. SHZ-1 was 30℃, and the activation energy of the hydration of NHase was 90.2kJ·mol^-1 in the temperature range from 5℃ to 30℃. Kmof NHase was 0.095mol·L^-1 using acrylonitrile（AN） as substrate, and NHase activity was inhibited seriously when acrylonitrile concentration was up to 40g·L^-1, the substrate inhibition constant Ki is 0.283mol·L^-1. Moreover, the NHase from Rhodococcus sp. SHZ-1 had very strong tolerance to acrylamide, in which the final concentration of acrylamide reached to 642g·L^-1 and the residual activity of NHase still maintained 8.6% of the initial enzyme activity.展开更多
目的观察抗抑郁治疗对老年脑卒中后抑郁(post-stoke depression,PSD)患者神经功能康复和神经元特异性烯醇化酶(neuron-specific enolase,NSE)的影响。方法选择2015年7月~2018年6月眉山市人民医院神经内科收治的老年脑卒中合并PSD患者119...目的观察抗抑郁治疗对老年脑卒中后抑郁(post-stoke depression,PSD)患者神经功能康复和神经元特异性烯醇化酶(neuron-specific enolase,NSE)的影响。方法选择2015年7月~2018年6月眉山市人民医院神经内科收治的老年脑卒中合并PSD患者119例,按照入院顺序随机双盲试验设计,分为治疗组60例和对照组59例,动态观察2组抗抑郁治疗前,治疗后2、4、8周及6个月美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分、汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评分和NSE水平的差异。结果 2组治疗后2、4、8周及6个月HAMD评分和NIHSS评分较治疗前明显降低,差异有统计学意义(P<0.05)。治疗组治疗后2、4、8周及6个月HAMD评分明显低于对照组,差异有统计学意义(P<0.01)。治疗组治疗后4、8周及6个月NIHSS评分明显低于对照组,差异有统计学意义(P<0.05,P<0.01)。治疗组治疗后2、4、8周血清NSE水平明显低于对照组,差异有统计学意义[(28.70±2.38)μg/L vs (30.78±2.72)μg/L,(24.16±2.45)μg/L vs (26.12±3.02)μg/L,(13.17±2.60)μg/L vs (15.94±3.39)μg/L,P<0.01]。Person相关性分析显示,治疗前血清NSE水平与NIHSS评分呈正相关(r=0.459,P<0.01)。结论抗抑郁治疗可改善老年PSD患者的抑郁症状,促进神经功能恢复,早期血清NSE水平可作为神经功能康复的预测指标。展开更多
文摘Objective: To elucidate the role of S-100B and neuron specific enolase (NSE) in predicting the outcomes of patients with severe head injury. Methods: Forty patients with severe head injury were included in this study. The serum concentrations of S-100B and NSE were measured within 12 hours after head injury to investigate the correlation between serum levels of S-100B and NSE and outcome. Validity of both S-100B and NSE in outcome prediction was assessed with Receiver Operator Characteristic (ROC) curve. Results: The serum concentrations of S-100B and NSE of both groups, with favorable or unfavorable outcomes, were significantly higher than those of the normal group. The serum concentrations within 12 hours after head injury were closely correlated with the prognosis. Furthermore, according to the ROC curves of S-100B and NSE, S-100B was found better in predicting outcomes than NSE. Conclusions: S-100B and NSE may play important roles in outcome prediction after severe head injury. Moreover, S-100B is clearly superior to NSE in terms of predictive value and appears to be a more promising serum marker in outcome prediction after severe head injury.
基金Supported by the Foundation for the Authors of National Excellent Doctoral Dissertation of China (200345)the National High Technology Research and Development Program of China (2007AA02Z201)the National Basic Research Program of China (2007CB714304)
文摘Nitrile hydratase (NHase) is an important industrial enzyme used for acrylamide production from acrylonitrile.The deactivation kinetics of NHases in free resting cells of Rhodococcus sp.was presented based on a bi-steady state assumption.Effects of hydration temperature,product concentration and substrate concentration on NHase deactivation were investigated experimentally and correlated with a first order deactivation kinetics.The results showed that the hydration temperature and product concentration were major factors governing the deactivation of NHases under substrate-feeding conditions.When acrylamide concentration was higher than 250 g·L1,the deactivation of NHases became serious and the bi-steady state assumption was not applicable.When the hydration temperature was controlled at a relatively higher level such as 28°C,the total deactivation rate constant was about 2.8-fold of that at 20°C.
文摘A new approach to optically active β-alkyl-α-methylene-γ-butyrolactone derivatives was reported from the Rhodococcus sp. AJ270-catalyzed hydrolysis of appropriate nitriles. The inversion of enantioselectivity of the amidase has been observed when a methyl protection was introduced into the hydroxy group of the parent substrate.
基金Project supported by the Major State Basic Research Development Program （No. 2003CB716005）, the Ministry of Science and Technology, the National Science Foundation of China, and the Chinese Academy of Sciences.
文摘Catalyzed by Rhodococcus erythropolis AJ270, a nitrile hydratase and amidase containing microbial whole-cell catalyst, at 10 ℃ and with the use of methanol as a co-solvent, nitrile and amide biotransformations produce 2S-1,4-benzodioxane-2-carboxamide and 2R-1,4-benzodioxane-2-carboxylic acid in high yields with excellent enantioselectivity.
文摘Researches in recent years have shown that cellular immune factor plays an important role in the generation and development of cerebral hemorrhage1-3.
基金Supported by the National Natural Science Foundation of China （No.20466002）, the Program for New Century Excellent Talents in University （NCET-04-089） and the Key Research Projects in the Uygur Autonomous Region of Xinjiang （No.200332109）.
文摘Inducing expression and the reaction characteristic of nitrile hydratase （NHase） from Rhodococcus sp. SHZ-1 were investigated. The results showed that the expression of NHase was greatly enhanced with the cooperation of acrylonitrile and ammonium chloride as inducer in the medium and the specific activity of NHase was increased of 44%. Then the temperature, pH, concentration of acrylonitrile and acrylamide were evaluated, which affected the activity and reaction characteristic of NHase. It was found that the temperature and concentration of acrylarnide were the most important factors for the catalyzation of NHase. The optimal catalysis temperature of NHase from Rhodococcus sp. SHZ-1 was 30℃, and the activation energy of the hydration of NHase was 90.2kJ·mol^-1 in the temperature range from 5℃ to 30℃. Kmof NHase was 0.095mol·L^-1 using acrylonitrile（AN） as substrate, and NHase activity was inhibited seriously when acrylonitrile concentration was up to 40g·L^-1, the substrate inhibition constant Ki is 0.283mol·L^-1. Moreover, the NHase from Rhodococcus sp. SHZ-1 had very strong tolerance to acrylamide, in which the final concentration of acrylamide reached to 642g·L^-1 and the residual activity of NHase still maintained 8.6% of the initial enzyme activity.
文摘目的观察抗抑郁治疗对老年脑卒中后抑郁(post-stoke depression,PSD)患者神经功能康复和神经元特异性烯醇化酶(neuron-specific enolase,NSE)的影响。方法选择2015年7月~2018年6月眉山市人民医院神经内科收治的老年脑卒中合并PSD患者119例,按照入院顺序随机双盲试验设计,分为治疗组60例和对照组59例,动态观察2组抗抑郁治疗前,治疗后2、4、8周及6个月美国国立卫生研究院卒中量表(national institute of health stroke scale,NIHSS)评分、汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评分和NSE水平的差异。结果 2组治疗后2、4、8周及6个月HAMD评分和NIHSS评分较治疗前明显降低,差异有统计学意义(P<0.05)。治疗组治疗后2、4、8周及6个月HAMD评分明显低于对照组,差异有统计学意义(P<0.01)。治疗组治疗后4、8周及6个月NIHSS评分明显低于对照组,差异有统计学意义(P<0.05,P<0.01)。治疗组治疗后2、4、8周血清NSE水平明显低于对照组,差异有统计学意义[(28.70±2.38)μg/L vs (30.78±2.72)μg/L,(24.16±2.45)μg/L vs (26.12±3.02)μg/L,(13.17±2.60)μg/L vs (15.94±3.39)μg/L,P<0.01]。Person相关性分析显示,治疗前血清NSE水平与NIHSS评分呈正相关(r=0.459,P<0.01)。结论抗抑郁治疗可改善老年PSD患者的抑郁症状,促进神经功能恢复,早期血清NSE水平可作为神经功能康复的预测指标。
