目的:探讨生物活性玻璃(biologically active glass,BAG)对重组人骨形成蛋白-2(recombinenthuman bone morphogenetic protein-2 rhBMP-2)诱导骨形成的的影响,评价rhBMP-2/BAG复合物在兔颅骨表面的骨扩增作用.方法:在16例兔模型的颅骨...目的:探讨生物活性玻璃(biologically active glass,BAG)对重组人骨形成蛋白-2(recombinenthuman bone morphogenetic protein-2 rhBMP-2)诱导骨形成的的影响,评价rhBMP-2/BAG复合物在兔颅骨表面的骨扩增作用.方法:在16例兔模型的颅骨骨膜下、骨皮质表面,分别植入rhBMP-2/BAG、BAG和单纯BMP,并设空白对照组.植入后2、4、8周行组织学检查,观察植骨区的变化,并测量成骨厚度.结果:rhBMP-2/BAG的表面成骨作用比单一BAG、rhBMP-2好,其骨化过程类似于膜内化骨的直接骨形成,与骨皮质表面良好结合,新骨中可见BAG颗粒并于8周后大部分吸收,新骨形成厚度明显高于BAG组,BAG组的新骨形成厚度明显高于其他两组.结论:BAG具有骨传导性能和可吸收性,rhBMP-2/BAG复合材料将骨诱导性和骨传导性结合起来,促进骨表面的扩增,是一种有临床应用前景的骨替代材料.展开更多
AIM:To analyze bone inductive capacity of the compound material of decalcified bone matrix combined with rhBMP 2 and impregnated with bone cement.METHODS:To assess the experimental study, histological and Masson’s me...AIM:To analyze bone inductive capacity of the compound material of decalcified bone matrix combined with rhBMP 2 and impregnated with bone cement.METHODS:To assess the experimental study, histological and Masson’s methods were used.RESULTS:The effects of compound material on the induction of bone formation were investigated in NIH mouse models.It was observed that in the with rhBMP 2 group, mesenchymal cells gathered in the implanted material at the 7th day postoperation,chondrogenesis were found at 14 to 21 days after implantation,new bone formation were observed at about 21 to 28 days after surgery and the DBM particles were absorbed by the new generated tissues gradually.CONCLUSIONS:The compound material of DBM combined with rhBMP 2 and impregnated with bone cement could induce the proliferation and migration of mesenchymal tissues that could be differentiated into cartilage and formed new bone finally.The new bone could absorb DBM particles gradually.The compound material had fair capacity of bone induction.展开更多
目的构建人骨形态发生蛋白2(BM P 2)与血管内皮生长因子(VEGF)双基因腺病毒穿梭质粒。方法对pCU-CAGG S-hVEGF 165的目的基因亚克隆并引入新的酶切位点,定向连入腺病毒穿梭载体pShu ttle-CM V,将质粒pcDNA 3.1-BM P 2及pIRES酶切后,将BM...目的构建人骨形态发生蛋白2(BM P 2)与血管内皮生长因子(VEGF)双基因腺病毒穿梭质粒。方法对pCU-CAGG S-hVEGF 165的目的基因亚克隆并引入新的酶切位点,定向连入腺病毒穿梭载体pShu ttle-CM V,将质粒pcDNA 3.1-BM P 2及pIRES酶切后,将BM P 2和IRES片段定向连入pShu ttle-CM V-hVEGF 165,构建可同时表达两个目的基因重组质粒pShu ttle-CM V-hBM P 2-IRES-hVEGF 165,进行酶切分析及序列测定。结果酶切分析及核酸序列测定证实重组质粒构建正确。结论成功构建了BM P 2及VEGF双基因腺病毒穿梭质粒;为联合基因治疗骨缺损的研究奠定了基础。展开更多
文摘目的:探讨生物活性玻璃(biologically active glass,BAG)对重组人骨形成蛋白-2(recombinenthuman bone morphogenetic protein-2 rhBMP-2)诱导骨形成的的影响,评价rhBMP-2/BAG复合物在兔颅骨表面的骨扩增作用.方法:在16例兔模型的颅骨骨膜下、骨皮质表面,分别植入rhBMP-2/BAG、BAG和单纯BMP,并设空白对照组.植入后2、4、8周行组织学检查,观察植骨区的变化,并测量成骨厚度.结果:rhBMP-2/BAG的表面成骨作用比单一BAG、rhBMP-2好,其骨化过程类似于膜内化骨的直接骨形成,与骨皮质表面良好结合,新骨中可见BAG颗粒并于8周后大部分吸收,新骨形成厚度明显高于BAG组,BAG组的新骨形成厚度明显高于其他两组.结论:BAG具有骨传导性能和可吸收性,rhBMP-2/BAG复合材料将骨诱导性和骨传导性结合起来,促进骨表面的扩增,是一种有临床应用前景的骨替代材料.
文摘AIM:To analyze bone inductive capacity of the compound material of decalcified bone matrix combined with rhBMP 2 and impregnated with bone cement.METHODS:To assess the experimental study, histological and Masson’s methods were used.RESULTS:The effects of compound material on the induction of bone formation were investigated in NIH mouse models.It was observed that in the with rhBMP 2 group, mesenchymal cells gathered in the implanted material at the 7th day postoperation,chondrogenesis were found at 14 to 21 days after implantation,new bone formation were observed at about 21 to 28 days after surgery and the DBM particles were absorbed by the new generated tissues gradually.CONCLUSIONS:The compound material of DBM combined with rhBMP 2 and impregnated with bone cement could induce the proliferation and migration of mesenchymal tissues that could be differentiated into cartilage and formed new bone finally.The new bone could absorb DBM particles gradually.The compound material had fair capacity of bone induction.
文摘目的构建人骨形态发生蛋白2(BM P 2)与血管内皮生长因子(VEGF)双基因腺病毒穿梭质粒。方法对pCU-CAGG S-hVEGF 165的目的基因亚克隆并引入新的酶切位点,定向连入腺病毒穿梭载体pShu ttle-CM V,将质粒pcDNA 3.1-BM P 2及pIRES酶切后,将BM P 2和IRES片段定向连入pShu ttle-CM V-hVEGF 165,构建可同时表达两个目的基因重组质粒pShu ttle-CM V-hBM P 2-IRES-hVEGF 165,进行酶切分析及序列测定。结果酶切分析及核酸序列测定证实重组质粒构建正确。结论成功构建了BM P 2及VEGF双基因腺病毒穿梭质粒;为联合基因治疗骨缺损的研究奠定了基础。
