Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in...Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke.展开更多
A cultivar trial, including commercial and heirloom cultivars of major cereals and grain legumes was conducted in Vancouver, BC, under low input organic conditions. We assessed 19 wheat (6 commercial and 13 heirloom),...A cultivar trial, including commercial and heirloom cultivars of major cereals and grain legumes was conducted in Vancouver, BC, under low input organic conditions. We assessed 19 wheat (6 commercial and 13 heirloom), 17 barley (8 commercial and 9 heirloom), 5 pea, 5 favabean, 5 kidneybean, 2 lentil, and 2 soyabean cultivars for plant performance metrics, and their potential in a small grain:legume intercropping system. Heirloom wheat cultivars showed notable response in a number of parameters including late maturity, taller plants, greatest number of spikes per m2, longest spike, highest number of seed per spike, greater seed weight to volume ratio, and resistance to stripe rust compared with commercial cultivars. For the heirloom-type, 6 of 14 wheat cultivars, (i.e., “Reward”, “Glenn”, “Cerebs”, “Red Bobs”, “Sounders” and “Black Bearded”) produced yields comparable to the commercial cultivars (i.e., nearly 5 t/h or higher). Also, heirloom cultivars typically contained higher protein levels most suitable for baking and blending purposes with “Einkorn” displaying the highest level (16.2%). Heirloom and commercial barley cultivars did not differ significantly with respect to plant height, spike length, and seed weight to volume ratio. However, a number of heirloom cultivars (e.g., “Jet”, “Dolma”, “Andie” and “Himalayan”) displayed greater responses on earliness, number of spikes per m2, grain yield, protein content and seed weight to volume ratio. Pea and lentil yielded lower than the national average under trial conditions. However, heirloom peas “Corgi”, “De Grace”, “Snowbird”, and “Golden” were earlier compared to the commercial cultivar “Reward”. All kidney bean cultivars yielded ~3 t/h with the highest yield from “Red Kidney” (3.8 t/h). Fava and soyabean appeared as promising crops as the cultivars produced good growth and yields. Neither lentil (“Crimson” and “Essex”) produced satisfactory responses though they had excellent vegetati展开更多
The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native ba...The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native barley varieties were chosen with the detection of 24 quality indexes in order to build a system of comprehensive evaluation.The results of the factor analysis indicated that seven common factors with an eigenvalue greater than 1 were extracted,cumulatively accounting for 96.21%of the total variance.The first common factor,including ASP,GLU,SER,GLY,ARG,TYR and CYS contents,accounted for 33.82% of the variance.The second common factor,including ash,the total starch,soluble fiber,VB_(3),Cu,Mn,Na and beta-glucan contents,accounted for 19.46%of the variance.The third common factor,including the total dietary fiber,α-VE,K,Zn and glutelin.The fourth common factor,including B,Ba and prolamin,explained the barley starch character of the rheological property.The fifth common factor included crude fiber.The sixth and the seventh common factors did not account for a substantial amount of variance.According to the comprehensive evaluation model,the score consequence was as the following:Zangqing25>Pengnaigabu>Lhasa changhei>2004Qing21>Lhasa duanbai>Liangamu>Zhikonggaxia>lianmubai>Jiangreejiu>Longzihei.展开更多
利用92个SSR标记对108份青稞亲本材料进行多态性扫描,分析其遗传多样性,旨在寻找与农艺性状相关联的分子标记,为青稞杂交组合的配制及分子标记辅助育种提供依据。挑选48个多态性标记进行群体遗传结构分析,在此基础上采用Tassel 2.1 GLM...利用92个SSR标记对108份青稞亲本材料进行多态性扫描,分析其遗传多样性,旨在寻找与农艺性状相关联的分子标记,为青稞杂交组合的配制及分子标记辅助育种提供依据。挑选48个多态性标记进行群体遗传结构分析,在此基础上采用Tassel 2.1 GLM（general linear model）和MLM（mixed linear model）方法进行标记与农艺性状的关联分析。共检测出156个等位变异,每个位点2～6个等位变异。供试群体的Shannon指数为0.6727～1.1368,材料间遗传相似系数为0.2250～1.0000,平均0.7585。通过群体遗传结构分析将供试材料划分成4个亚群。以GLM分析,发现12个与株高、穗长、穗粒数和分蘖数相关联的标记,对表型变异的解释率分别为11.5%～17.6%、19.4%～45.4%、15.4%～22.1%和29.2%;以MLM分析,发现8个与株高、分蘖数和小穗数相关的标记,各标记对表型变异的解释率分别为31.7%～49.8%、28.1%～37.2%、22.7%～32.7%。关联标记分布在基因组全部6个连锁群上。展开更多
基金This research was supported by the following funding sources:the Tibet Autonomous Region Financial Special Fund 2015CZZX001,2014CZZXQ01,2015ZX001,2017CZZX001/2,and XZNKY-2018-C-021,and the National Key Research Project Fund,China 2018YFD100070 and 2018YFD1000703.
文摘Qingke (Tibetan hulless barley) has long been cultivated and exposed to long-term and strong UV-B radiation on the Tibetan Plateau, which renders it an ideal species for elucidating novel UV-B responsive mechanisms in plants. Here we report a comprehensive metabolite profiling and metabolite-based genome-wide association study (mGWAS) using 196 diverse qingke and barley accessions. Our results demonstrated both constitutive and induced accumulation, and common genetic regulation, of metabolites from different branches of the phenylpropanoid pathway that are involved in UV-B protection. A total of 90 significant mGWAS loci for these metabolites were identified in barley-qingke differentiation regions, and a number of high-level metabolite trait alleles were found to be significantly enriched in qingke, suggesting co-selection of various phenylpropanoids. Upon dissecting the entire phenylpropanoid pathway, we identified some key determinants controlling natural variation of phenylpropanoid content, including three novel proteins, a flavone C-pentosyltransferase, a tyramine hydroxycinnamoyl acyltransferase, and a MYB transcription factor. Our study, furthermore, demonstrated co-selection of both constitutive and induced phenylpropanoids for UV-B protection in qingke.
文摘A cultivar trial, including commercial and heirloom cultivars of major cereals and grain legumes was conducted in Vancouver, BC, under low input organic conditions. We assessed 19 wheat (6 commercial and 13 heirloom), 17 barley (8 commercial and 9 heirloom), 5 pea, 5 favabean, 5 kidneybean, 2 lentil, and 2 soyabean cultivars for plant performance metrics, and their potential in a small grain:legume intercropping system. Heirloom wheat cultivars showed notable response in a number of parameters including late maturity, taller plants, greatest number of spikes per m2, longest spike, highest number of seed per spike, greater seed weight to volume ratio, and resistance to stripe rust compared with commercial cultivars. For the heirloom-type, 6 of 14 wheat cultivars, (i.e., “Reward”, “Glenn”, “Cerebs”, “Red Bobs”, “Sounders” and “Black Bearded”) produced yields comparable to the commercial cultivars (i.e., nearly 5 t/h or higher). Also, heirloom cultivars typically contained higher protein levels most suitable for baking and blending purposes with “Einkorn” displaying the highest level (16.2%). Heirloom and commercial barley cultivars did not differ significantly with respect to plant height, spike length, and seed weight to volume ratio. However, a number of heirloom cultivars (e.g., “Jet”, “Dolma”, “Andie” and “Himalayan”) displayed greater responses on earliness, number of spikes per m2, grain yield, protein content and seed weight to volume ratio. Pea and lentil yielded lower than the national average under trial conditions. However, heirloom peas “Corgi”, “De Grace”, “Snowbird”, and “Golden” were earlier compared to the commercial cultivar “Reward”. All kidney bean cultivars yielded ~3 t/h with the highest yield from “Red Kidney” (3.8 t/h). Fava and soyabean appeared as promising crops as the cultivars produced good growth and yields. Neither lentil (“Crimson” and “Essex”) produced satisfactory responses though they had excellent vegetati
基金Supported by Chnia Agriculture Research Systemthe Scientific Research Fund of the Key Technology and Research and Development of Barley Characteristic Agricultural Products Processing(XZ201901NA04)Development and Industrialization Application of Xizang Highland Barley Baijiu(XZ202001ZY0017N)。
文摘The purpose of this study was to explore the quality of the Tibetan native hulless barley variety in depth and to evaluate the characteristics of its processing quality using ratio analysis.For this study,10 native barley varieties were chosen with the detection of 24 quality indexes in order to build a system of comprehensive evaluation.The results of the factor analysis indicated that seven common factors with an eigenvalue greater than 1 were extracted,cumulatively accounting for 96.21%of the total variance.The first common factor,including ASP,GLU,SER,GLY,ARG,TYR and CYS contents,accounted for 33.82% of the variance.The second common factor,including ash,the total starch,soluble fiber,VB_(3),Cu,Mn,Na and beta-glucan contents,accounted for 19.46%of the variance.The third common factor,including the total dietary fiber,α-VE,K,Zn and glutelin.The fourth common factor,including B,Ba and prolamin,explained the barley starch character of the rheological property.The fifth common factor included crude fiber.The sixth and the seventh common factors did not account for a substantial amount of variance.According to the comprehensive evaluation model,the score consequence was as the following:Zangqing25>Pengnaigabu>Lhasa changhei>2004Qing21>Lhasa duanbai>Liangamu>Zhikonggaxia>lianmubai>Jiangreejiu>Longzihei.
文摘利用92个SSR标记对108份青稞亲本材料进行多态性扫描,分析其遗传多样性,旨在寻找与农艺性状相关联的分子标记,为青稞杂交组合的配制及分子标记辅助育种提供依据。挑选48个多态性标记进行群体遗传结构分析,在此基础上采用Tassel 2.1 GLM（general linear model）和MLM（mixed linear model）方法进行标记与农艺性状的关联分析。共检测出156个等位变异,每个位点2～6个等位变异。供试群体的Shannon指数为0.6727～1.1368,材料间遗传相似系数为0.2250～1.0000,平均0.7585。通过群体遗传结构分析将供试材料划分成4个亚群。以GLM分析,发现12个与株高、穗长、穗粒数和分蘖数相关联的标记,对表型变异的解释率分别为11.5%～17.6%、19.4%～45.4%、15.4%～22.1%和29.2%;以MLM分析,发现8个与株高、分蘖数和小穗数相关的标记,各标记对表型变异的解释率分别为31.7%～49.8%、28.1%～37.2%、22.7%～32.7%。关联标记分布在基因组全部6个连锁群上。
