Normal mammalian secondary palate development undergoes a series of processes,including palatal shelf(PS)growth,elevation,adhesion and fusion,and palatal bone formation.It has been estimated that more than 90%of isola...Normal mammalian secondary palate development undergoes a series of processes,including palatal shelf(PS)growth,elevation,adhesion and fusion,and palatal bone formation.It has been estimated that more than 90%of isolated cleft palate is caused by defects associated with the elevation process.However,because of the rapidly completed elevation process,the entire process of elevation will never be easy to clarify.In this article,we present a novel method for three-dimensional(3D)reconstruction of thick tissue blocks from two-dimensional(2D)histological sections.We established multiplanar sections of the palate and tongue in coronal and sagittal directions,and further performed 3D reconstruction to observe the morphological interaction and connection between the two components prior to and during elevation.The method completes an imaging system for simultaneous morphological analysis of thick tissue samples using both synthetic and real data.The new method will provide a comprehensive picture of reorientation morphology and gene expression pattern during the palatal elevation process.展开更多
Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary ...Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary information about tissue microstructure, displaying distinctive patterns between normal and malignant human colon. Additionally, we have demonstrated the usefulness of using fixed tissues for the disease diagnostic and prognostic.?The present work provides a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer. NLO metrics could be applied to other disorders, which are characterized by abnormal cell proliferation and collagen assembly.展开更多
The efficacies of some indigenous herbal dyes for use in staining plant materials were examined to obtain non-toxic, eco-friendly and cheap stains for use in plant histology. Dye extracts from Bixa orellana, Curcuma d...The efficacies of some indigenous herbal dyes for use in staining plant materials were examined to obtain non-toxic, eco-friendly and cheap stains for use in plant histology. Dye extracts from Bixa orellana, Curcuma domestica, Lonchocarpus cyanescens and Pterocarpus osun were used to stain wood sections using the existing standard staining procedures with little modification. All the extracts had affinity for the fibre and vessel elements except the extract from L. cyanescens. The extracts from C. domestica and B. orellana had higher selectivity than those ofP. osun for fibre. From the results of the absorbance curves, each of the dye extracts from all speciese had minimum of two peaks, indicating that they had two or more colour imparting chromophores except dye extract from C. domestica. All the dye extracts were acidic with pH range of 3.77 to 6.77. Therefore, this study shows that dye extracts from B. orellana, C. domestica and P. osun could be solitarily or in combination with artificial dyes for plant histological staining.展开更多
The following experiments indicated that under a specified condition oral and esophageal cancer could be affected selectively by YAG Laser. (1) Oral cavity cancer and the surrounding normal tissues were irradiated sim...The following experiments indicated that under a specified condition oral and esophageal cancer could be affected selectively by YAG Laser. (1) Oral cavity cancer and the surrounding normal tissues were irradiated simultaneously by YAG Laser under a specified condition. A selective effect could be seen obviously under the naked eye observation and histological sections.展开更多
基金National Natural Science Foundation of China(81400494)the Key Program of Science&Technology Department of Sichuan Province,China(2019ZDYF1658,and 2019ZDYF1430).
文摘Normal mammalian secondary palate development undergoes a series of processes,including palatal shelf(PS)growth,elevation,adhesion and fusion,and palatal bone formation.It has been estimated that more than 90%of isolated cleft palate is caused by defects associated with the elevation process.However,because of the rapidly completed elevation process,the entire process of elevation will never be easy to clarify.In this article,we present a novel method for three-dimensional(3D)reconstruction of thick tissue blocks from two-dimensional(2D)histological sections.We established multiplanar sections of the palate and tongue in coronal and sagittal directions,and further performed 3D reconstruction to observe the morphological interaction and connection between the two components prior to and during elevation.The method completes an imaging system for simultaneous morphological analysis of thick tissue samples using both synthetic and real data.The new method will provide a comprehensive picture of reorientation morphology and gene expression pattern during the palatal elevation process.
文摘Combined multimodal nonlinear optical (NLO) microscopies were used to detect and quantify morphological changes associated with stroma and epithelial transformation in colon cancer. Our findings provide complementary information about tissue microstructure, displaying distinctive patterns between normal and malignant human colon. Additionally, we have demonstrated the usefulness of using fixed tissues for the disease diagnostic and prognostic.?The present work provides a framework for using NLO techniques as a clinical diagnostic tool for human colon cancer. NLO metrics could be applied to other disorders, which are characterized by abnormal cell proliferation and collagen assembly.
文摘The efficacies of some indigenous herbal dyes for use in staining plant materials were examined to obtain non-toxic, eco-friendly and cheap stains for use in plant histology. Dye extracts from Bixa orellana, Curcuma domestica, Lonchocarpus cyanescens and Pterocarpus osun were used to stain wood sections using the existing standard staining procedures with little modification. All the extracts had affinity for the fibre and vessel elements except the extract from L. cyanescens. The extracts from C. domestica and B. orellana had higher selectivity than those ofP. osun for fibre. From the results of the absorbance curves, each of the dye extracts from all speciese had minimum of two peaks, indicating that they had two or more colour imparting chromophores except dye extract from C. domestica. All the dye extracts were acidic with pH range of 3.77 to 6.77. Therefore, this study shows that dye extracts from B. orellana, C. domestica and P. osun could be solitarily or in combination with artificial dyes for plant histological staining.
文摘The following experiments indicated that under a specified condition oral and esophageal cancer could be affected selectively by YAG Laser. (1) Oral cavity cancer and the surrounding normal tissues were irradiated simultaneously by YAG Laser under a specified condition. A selective effect could be seen obviously under the naked eye observation and histological sections.
