由尖孢镰刀菌西瓜专化型(Fusarium oxysporum f. sp. niveum,FON)引起的西瓜枯萎病是一种毁灭性的土传病害。本研究通过离体及活体试验,测定了植物源天然抑菌活性物质桧木醇对西瓜枯萎病菌的抑制活性、对西瓜枯萎病的盆栽防效以及对病...由尖孢镰刀菌西瓜专化型(Fusarium oxysporum f. sp. niveum,FON)引起的西瓜枯萎病是一种毁灭性的土传病害。本研究通过离体及活体试验,测定了植物源天然抑菌活性物质桧木醇对西瓜枯萎病菌的抑制活性、对西瓜枯萎病的盆栽防效以及对病原菌生理生化指标的影响。结果表明:桧木醇对西瓜枯萎病菌具有明显的抑制效果,其对病原菌菌丝生长和孢子萌发的抑制作用EC_(50)值分别为31.1和45.2μg/mL;在250、500和1000μg/mL 3个质量浓度下,桧木醇对西瓜枯萎病均有显著的防治效果,其中1000μg/mL时防效可达75.2%,与对照药剂多菌灵500μg/mL的效果相当。生理生化指标测定及外观形态观察发现,经桧木醇处理后,西瓜枯萎病菌菌丝相对电导率上升,菌丝体内镰刀菌酸含量显著降低,菌丝呈现扭曲、畸形、缠绕等不规则外观形态。研究表明,桧木醇不仅能显著抑制西瓜枯萎病菌的生长,同时能抑制其相关毒素的生物合成或促进其代谢,从而降低病原菌的致病力,具有开发为西瓜枯萎病防治替代药剂的潜力。桧木醇处理能破坏病原菌细胞膜的完整性、干扰病原菌次生代谢物的合成或代谢过程,但其具体的抑菌分子靶标仍需进一步研究。展开更多
Hinokitiolis frequently added to personal care products as an antibacterial agent. We previously established an HPLC-UV method for determination of hinokitiol in skin lotion after pre-column derivatization with 4-fluo...Hinokitiolis frequently added to personal care products as an antibacterial agent. We previously established an HPLC-UV method for determination of hinokitiol in skin lotion after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole. However, the labeling reagent is expensive, and derivatives of degradation products of parabens, which may be added to skin lotion as general preservatives, interfered with the peak of the hinokitiol derivative. In this study, the concentration of hinokitiol in skin lotions was determined by HPLC with a visible light detector (450 nm) after pre-column derivatization with 4-(dimethylamino)azobenzene-4’-sulfonyl chloride (Dabsyl-Cl), a more economical reagent. A standard curve was obtained after derivatization with Dabsyl-Cl in borate buffer (pH 9.5) at 55°C for 10 min. The retention time of Dabsyl-hinokitiol was 6.8 min. The calibration plot was linear in the range of 1.25 to 40 μg/mL with a r2 value of 0.9991, and the lower limit of quantification and detection were 0.60 μg/mL (absolute amount of 0.86 ng/20μL injection, signal-to-noise ratio of 10:1) and 0.18 μg/mL (absolute amount of 0.26 ng/20μL injection, signal-to-noise ratio of 3:1), respectively. The coefficient of variation was less than 8.8%. Seven Dabsyl-paraben derivatives showed little interference with the peak of Dabsyl-hinokitiol. The developed system was used to determine the content of hinokitiol in two skin lotions. Addition-recovery tests gave satisfactory results.展开更多
Hair loss and gray hair are the ultimate manifestations of scalp and hair aging.Improving metabolic activities in cells is a fundamental for anti-aging while autophagy activity of cell is a key measure.Hinokitiol,one ...Hair loss and gray hair are the ultimate manifestations of scalp and hair aging.Improving metabolic activities in cells is a fundamental for anti-aging while autophagy activity of cell is a key measure.Hinokitiol,one of the more than 30 quasi-drug actives approved in Japan in the field of hair tonic products has two known mechanisms for its effectivities,i.e.promoting cell activity and anti-microbial.Through this study,we identified a new mechanism of hinokitiol for hair loss prevention,i.e.promotion of autophagy and mitophagy activities of dermal papilla cells.展开更多
文摘Hinokitiolis frequently added to personal care products as an antibacterial agent. We previously established an HPLC-UV method for determination of hinokitiol in skin lotion after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole. However, the labeling reagent is expensive, and derivatives of degradation products of parabens, which may be added to skin lotion as general preservatives, interfered with the peak of the hinokitiol derivative. In this study, the concentration of hinokitiol in skin lotions was determined by HPLC with a visible light detector (450 nm) after pre-column derivatization with 4-(dimethylamino)azobenzene-4’-sulfonyl chloride (Dabsyl-Cl), a more economical reagent. A standard curve was obtained after derivatization with Dabsyl-Cl in borate buffer (pH 9.5) at 55°C for 10 min. The retention time of Dabsyl-hinokitiol was 6.8 min. The calibration plot was linear in the range of 1.25 to 40 μg/mL with a r2 value of 0.9991, and the lower limit of quantification and detection were 0.60 μg/mL (absolute amount of 0.86 ng/20μL injection, signal-to-noise ratio of 10:1) and 0.18 μg/mL (absolute amount of 0.26 ng/20μL injection, signal-to-noise ratio of 3:1), respectively. The coefficient of variation was less than 8.8%. Seven Dabsyl-paraben derivatives showed little interference with the peak of Dabsyl-hinokitiol. The developed system was used to determine the content of hinokitiol in two skin lotions. Addition-recovery tests gave satisfactory results.
文摘Hair loss and gray hair are the ultimate manifestations of scalp and hair aging.Improving metabolic activities in cells is a fundamental for anti-aging while autophagy activity of cell is a key measure.Hinokitiol,one of the more than 30 quasi-drug actives approved in Japan in the field of hair tonic products has two known mechanisms for its effectivities,i.e.promoting cell activity and anti-microbial.Through this study,we identified a new mechanism of hinokitiol for hair loss prevention,i.e.promotion of autophagy and mitophagy activities of dermal papilla cells.
