Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field...Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field eels(Monopterus albus Zuiew).Each bivalent has been idendified and a total of 310—450 bands on 12 bivalents observed.This might bethe largest number of multiple bands obtained from fish chromosomes so far published.An idiogram of 372G-bands has been set up and detailed descriptions of the landmark systems and the band positions have beengiven.展开更多
In the past few years, clear and abundant G-bands in more than ten plants have been successfully observed by use of animal and human G-banding procedures, which mainly include trypsin, ASG, AMD, urea, SDS, NaOH, NaHCO...In the past few years, clear and abundant G-bands in more than ten plants have been successfully observed by use of animal and human G-banding procedures, which mainly include trypsin, ASG, AMD, urea, SDS, NaOH, NaHCO<sub>3</sub> and Utakoji methods etc. However, all G-banding production methods have direct relations with the chemical factors. Although there is usually heat treatment （about 60℃） prior to the G-banding procedure in animal and human chromosomes, G-banding patterns have not yet been reported only by specif-展开更多
基金the National Doctorate Research Foundation of Chinathe National Natural Science Foundation of China
文摘Well spread pachytene bivalents and high resolution G-handing patterns have been obtainedby using the prolonged hypotonic treatment combined with the trypsin-hot salt banding technique in the sper-maries of rice field eels(Monopterus albus Zuiew).Each bivalent has been idendified and a total of 310—450 bands on 12 bivalents observed.This might bethe largest number of multiple bands obtained from fish chromosomes so far published.An idiogram of 372G-bands has been set up and detailed descriptions of the landmark systems and the band positions have beengiven.
基金Project supported by the National Natural Science Foundation of China.
文摘In the past few years, clear and abundant G-bands in more than ten plants have been successfully observed by use of animal and human G-banding procedures, which mainly include trypsin, ASG, AMD, urea, SDS, NaOH, NaHCO<sub>3</sub> and Utakoji methods etc. However, all G-banding production methods have direct relations with the chemical factors. Although there is usually heat treatment （about 60℃） prior to the G-banding procedure in animal and human chromosomes, G-banding patterns have not yet been reported only by specif-
