Background： Cytokines play an important role in occorrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to investigate the changes ofcytokines concentration and its correlation to al...Background： Cytokines play an important role in occorrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to investigate the changes ofcytokines concentration and its correlation to alanine aminotransferase （ALT）, HBV deoxyribonucleic acid （HBV-DNA）, hepatitis B envelope antigen （HBeAg）, and HBV surface antigen （HBsAg） in the development of chronic hepatitis B （CHB）. Methods： Thirteen healthy individuals （HI）, 30 chronic HBV-infected patients in immune tolerant （IT） phase, and 55 CHB patients were enrolled between August 2015 and May 2017. The peripheral blood samples were collected from all individuals. Tile levels of interferon （IFN）-α2, interleukin （IL）-10, transforming growth factor （TGF）-β1, HBV-DNA, HBsAg, and HBeAg and liver function were measured. The quantitative determinations of cytokines levels, including I FN-ct2, IL-10, and TGF-[31 were performed using Luminex multiplex technology. The correlation of cytokines to ALT, HBV-DNA, HBsAg, and HBeAg was analyzed by linear regression analysis. Results： IFN-ct2 levels were similar between HI and IT groups （15.35 [5.70, 67.65] pg/ml vs. 15.24 [4.07, 30.73] pg/ml, Z = -0.610, P - 0.542）, while it elevated significantly in CH B group （35.29 [ 15.94, 70.15] pg/ml vs. 15.24 [4.07, 30.73] pg/ml; Z = -2.522, P = 0.012）. Compared with HI group （3.73 [2.98, 11.92] pg/ml）, IL-10 concentrations in IT group （5.02 [2,98, 10.11] pg/ml）, and CHB group （7.48 [3. I 0, 18.00] pg/ml） slightly increased （X^2 = 2.015, P - 0.365）, and there was no significant difference between IT and CHB group （Z =- 1.419, P = 0.156）. The TGF-β1 levels among HI （3.59 ±0.20 pg/ml）, IT （3.62 ±0.55 pg/ml）, and CHB groups （3.64±0.30 pg/ml） were similar （X^2=2.739, P = 0.254）. In all chronic HBV-infected patients （including patients in IT and CHB groups）, the elevation of IFN-α2 level was significantly associated with ALT level （β = 0.389, t = 2.423, P = 0.018）,展开更多
目的 检测慢性乙型肝炎病毒(HBV)感染患者不同感染状态血清程序性细胞死亡因子4(PDCD4)信使核糖核酸(m RNA)表达水平,分析其与肝功能、HBV-脱氧核糖核酸(DNA)载量的相关性。方法 选择2020年6月-2021年7月本院收治的慢性HBV感染患者112例...目的 检测慢性乙型肝炎病毒(HBV)感染患者不同感染状态血清程序性细胞死亡因子4(PDCD4)信使核糖核酸(m RNA)表达水平,分析其与肝功能、HBV-脱氧核糖核酸(DNA)载量的相关性。方法 选择2020年6月-2021年7月本院收治的慢性HBV感染患者112例,根据HBV感染状态分为肝硬化组23例、慢性HBV组35例、慢性HBV携带组26例、非活动性乙型肝炎表面抗原(HBs Ag)携带组28例。选取本院健康体检者120例为对照组。检测受试者血清PDCD4 m RNA、HBs Ag、乙型肝炎表面抗体(HBs Ab)、e抗原(HBe Ag)、e抗体(HBe Ab)、核心抗体(HBc Ab)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、胆红素(BIL)、HBV-DNA载量水平;Pearson法分析血清PDCD4 m RNA与ALT、AST、BIL、HBV-DNA载量水平的相关性。结果 与对照组相比,非活动性HBs Ag携带组、慢性HBV携带组、慢性HBV组、肝硬化组血清PDCD4 m RNA表达水平显著下降,ALT、AST、BIL水平、HBV-DNA载量显著升高(P <0.05);随着感染程度的加深,PDCD4 m RNA表达水平呈现下降趋势,ALT、AST、BIL水平、HBV-DNA载量呈现一定的升高趋势(P <0.05);慢性HBV感染患者血清PDCD4 m RNA与ALT、AST、BIL水平及HBV-DNA载量之间呈负相关(P <0.05)。结论 慢性HBV感染患者血清PDCD4 m RNA呈低表达,且感染程度越深,PDCD4 m RNA表达水平越低,PDCD4 m RNA可能成为评判乙肝严重程度的生物标志物。展开更多
基金The work was supported by grants from the Basic and Clinical Fund of Capital Medical University （No. 17JL88） and National Natural Science Foundation of China （No. 81071344）.
文摘Background： Cytokines play an important role in occorrence and recovery of hepatitis B virus （HBV） infection. The aim of this study was to investigate the changes ofcytokines concentration and its correlation to alanine aminotransferase （ALT）, HBV deoxyribonucleic acid （HBV-DNA）, hepatitis B envelope antigen （HBeAg）, and HBV surface antigen （HBsAg） in the development of chronic hepatitis B （CHB）. Methods： Thirteen healthy individuals （HI）, 30 chronic HBV-infected patients in immune tolerant （IT） phase, and 55 CHB patients were enrolled between August 2015 and May 2017. The peripheral blood samples were collected from all individuals. Tile levels of interferon （IFN）-α2, interleukin （IL）-10, transforming growth factor （TGF）-β1, HBV-DNA, HBsAg, and HBeAg and liver function were measured. The quantitative determinations of cytokines levels, including I FN-ct2, IL-10, and TGF-[31 were performed using Luminex multiplex technology. The correlation of cytokines to ALT, HBV-DNA, HBsAg, and HBeAg was analyzed by linear regression analysis. Results： IFN-ct2 levels were similar between HI and IT groups （15.35 [5.70, 67.65] pg/ml vs. 15.24 [4.07, 30.73] pg/ml, Z = -0.610, P - 0.542）, while it elevated significantly in CH B group （35.29 [ 15.94, 70.15] pg/ml vs. 15.24 [4.07, 30.73] pg/ml; Z = -2.522, P = 0.012）. Compared with HI group （3.73 [2.98, 11.92] pg/ml）, IL-10 concentrations in IT group （5.02 [2,98, 10.11] pg/ml）, and CHB group （7.48 [3. I 0, 18.00] pg/ml） slightly increased （X^2 = 2.015, P - 0.365）, and there was no significant difference between IT and CHB group （Z =- 1.419, P = 0.156）. The TGF-β1 levels among HI （3.59 ±0.20 pg/ml）, IT （3.62 ±0.55 pg/ml）, and CHB groups （3.64±0.30 pg/ml） were similar （X^2=2.739, P = 0.254）. In all chronic HBV-infected patients （including patients in IT and CHB groups）, the elevation of IFN-α2 level was significantly associated with ALT level （β = 0.389, t = 2.423, P = 0.018）,
文摘目的 检测慢性乙型肝炎病毒(HBV)感染患者不同感染状态血清程序性细胞死亡因子4(PDCD4)信使核糖核酸(m RNA)表达水平,分析其与肝功能、HBV-脱氧核糖核酸(DNA)载量的相关性。方法 选择2020年6月-2021年7月本院收治的慢性HBV感染患者112例,根据HBV感染状态分为肝硬化组23例、慢性HBV组35例、慢性HBV携带组26例、非活动性乙型肝炎表面抗原(HBs Ag)携带组28例。选取本院健康体检者120例为对照组。检测受试者血清PDCD4 m RNA、HBs Ag、乙型肝炎表面抗体(HBs Ab)、e抗原(HBe Ag)、e抗体(HBe Ab)、核心抗体(HBc Ab)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、胆红素(BIL)、HBV-DNA载量水平;Pearson法分析血清PDCD4 m RNA与ALT、AST、BIL、HBV-DNA载量水平的相关性。结果 与对照组相比,非活动性HBs Ag携带组、慢性HBV携带组、慢性HBV组、肝硬化组血清PDCD4 m RNA表达水平显著下降,ALT、AST、BIL水平、HBV-DNA载量显著升高(P <0.05);随着感染程度的加深,PDCD4 m RNA表达水平呈现下降趋势,ALT、AST、BIL水平、HBV-DNA载量呈现一定的升高趋势(P <0.05);慢性HBV感染患者血清PDCD4 m RNA与ALT、AST、BIL水平及HBV-DNA载量之间呈负相关(P <0.05)。结论 慢性HBV感染患者血清PDCD4 m RNA呈低表达,且感染程度越深,PDCD4 m RNA表达水平越低,PDCD4 m RNA可能成为评判乙肝严重程度的生物标志物。
