Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three m...Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three months after radiation, neuronal injury at the T9-10 levels was observed, including reversible injury induced by spinal image-guided radiation therapy and apoptosis induced by conventional radiation therapy. The number of apoptotic cells and expression of the proapoptotic protein Fas were significantly reduced, but expression of the anti-apoptotic protein heat shock protein 70 was significantly increased after image-guided radiation therapy compared with the conventional method of the same radiation dose. Moreover, the spinal cord cell apoptotic index positively correlated with the ratio of Fas/heat shock protein 70. These findings indicate that 3 months of radiation therapy can induce a late response in the spinal cord to radiation therapy; image-guided radiation therapy is safer and results in less neuronal injury compared with conventional radiation therapy.展开更多
目的分析乙肝肝硬化患者血清乙型肝炎病毒脱氧核糖核酸(Hepatitis B Virus-Deoxyribonucleic Acid,HBV-DNA)载量与热休克蛋白70(Heat shock protein 70,Hsp70)、癌胚抗原(Carcino-embryonic antigen,CEA)及甲胎蛋白(α-fetoprotein,AFP...目的分析乙肝肝硬化患者血清乙型肝炎病毒脱氧核糖核酸(Hepatitis B Virus-Deoxyribonucleic Acid,HBV-DNA)载量与热休克蛋白70(Heat shock protein 70,Hsp70)、癌胚抗原(Carcino-embryonic antigen,CEA)及甲胎蛋白(α-fetoprotein,AFP)水平的相关性。方法将2016年5月-2019年5月在黄冈市中心医院消化内科就诊且符合纳入与排除标准的120例乙肝肝硬化患者作为研究对象,分别应用荧光定量PCR法检测HBV-DNA载量,酶联免疫吸附法(Enzyme-linked immunosorbent assay,ELISA)检测血清HSP70、CEA、AFP水平;比较不同HBV-DNA载量的乙肝肝硬化患者血清HSP70、CEA及AFP水平;并应用协方差分析校正混杂因素影响,Pearson相关性分析血清HBV-DNA载量与校正后的HSP70、CEA及AFP水平的相关性。结果 120例乙肝肝硬化患者中,HBV-DNA载量<5log10拷贝/ml 64例,平均(3.65±0.29)log10拷贝/ml;≥5log10拷贝/ml 56例,平均(5.98±0.28)log10拷贝/ml;其中HBV-DNA载量≥5log10拷贝/ml的乙肝肝硬化患者HSP70低于HBV-DNA载量<5log10拷贝/ml患者,CEA、AFP均高于HBV-DNA载量<5log10拷贝/ml患者(P<0.05);校正后不同HBV-DNA载量的乙肝肝硬化患者HSP70、CEA、AFP水平经Bonferroni检验差异仍有统计学意义(P<0.05);Pearson相关性分析显示HBV-DNA载量与HSP70、CEA、AFP均有显著相关性(r=-0.525、0.637、0.409,均P<0.01)。结论乙肝肝硬化患者血清HBV-DNA载量与HSP70显著负相关,与CEA、AFP呈正相关关系。展开更多
Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline ...Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.展开更多
植物热激蛋白HSP70(heat shock protein 70)与植物抗逆性具有密切的关系,对其深入研究将有助于揭示植物的抗逆生理过程,从而为生产上降低逆境对植物的伤害提供重要的实践指导意义。目前,在模式植物拟南芥和水稻中,HSP70蛋白家族的遗传...植物热激蛋白HSP70(heat shock protein 70)与植物抗逆性具有密切的关系,对其深入研究将有助于揭示植物的抗逆生理过程,从而为生产上降低逆境对植物的伤害提供重要的实践指导意义。目前,在模式植物拟南芥和水稻中,HSP70蛋白家族的遗传学和生化功能研究被广泛关注,但该基因家族植物中的系统分子进化、表达模式以及功能预测方面的研究仍不完善。本研究通过收集和整理拟南芥和水稻中所有的HSP70基因序列、基因结构和染色体定位等信息,对比分析了拟南芥和水稻中HSP70家族在分子进化方面的差异;同时,从转录水平上初步分析和阐明该家族基因在植物不同组织部位和不同发育时期的表达差异和功能分化。此外,本研究还围绕花药发育的过程,细致分析了HSP70家族基因的表达变化,推测该家族在花药不同时期可能所扮演的角色。本研究为全面理解HSP70家族在单子叶和双子叶植物中的功能异同分化、以及其参与植物生殖发育过程的功能研究提供重要的科学依据。展开更多
基金supported by the National Natural Science Foundation of China,No.81060182the Natural Science Foundation of Xinjiang Uygur Autonomous Region,No.2012211B34the Key Technology Research and Development and Major Program of Xinjiang Uygur Autonomous Region,No.200833116
文摘Tumor models were simulated in purebred Beagles at the T9-10 levels of the spinal cord and treated with spinal image-guided radiation therapy or conventional radiation therapy with 50 or 70 Gy total radiation. Three months after radiation, neuronal injury at the T9-10 levels was observed, including reversible injury induced by spinal image-guided radiation therapy and apoptosis induced by conventional radiation therapy. The number of apoptotic cells and expression of the proapoptotic protein Fas were significantly reduced, but expression of the anti-apoptotic protein heat shock protein 70 was significantly increased after image-guided radiation therapy compared with the conventional method of the same radiation dose. Moreover, the spinal cord cell apoptotic index positively correlated with the ratio of Fas/heat shock protein 70. These findings indicate that 3 months of radiation therapy can induce a late response in the spinal cord to radiation therapy; image-guided radiation therapy is safer and results in less neuronal injury compared with conventional radiation therapy.
文摘Objective To observe the expression of HSP70 mRNA in the rat induced by glutamine.Methods ①Twenty four healthy Sprague-Dawley rats were randomly divided into four groups（n=6 in each group）: groupⅠ received saline i.v.;group Ⅱ received glutamine（Gln） 0.15 g·kg-1 i.v.;group Ш received Gln 0.45 g·kg-1 i.v.;group Ⅳ received Gln 0.75 g·kg-1 i.v..All rats were killed after 4 h administration of Gln.HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.② Thirty healthy Sprague-Dawley rats were randomly divided into five groups（n=6 in each group）.They were killed at 1 h（group 1 h）、6 h（group 6 h）、12 h（group 12 h）、24 h（group 24 h） and 48 h（group 48 h） after administration of Gln(0.75 g·kg-1).HSP70 mRNA from heart、liver、lung and aorta as well as GAPDH mRNA were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results ① The expression of HSP70 mRNA in group Ⅱ、Ш and Ⅳ were higher than groupⅠ（P<0.05）,and the expression in groupⅡ、Ш and Ⅳ increased gradually.②The expression of HSP70 mRNA in group 1 h、 6 h and 12 h was higher than that in 24 h and 48h（P<0.05）.Conclusion Glutamine induces the expression of HSP70 mRNA in rats,and it occurs with the manner of dose and time dependent.
文摘植物热激蛋白HSP70(heat shock protein 70)与植物抗逆性具有密切的关系,对其深入研究将有助于揭示植物的抗逆生理过程,从而为生产上降低逆境对植物的伤害提供重要的实践指导意义。目前,在模式植物拟南芥和水稻中,HSP70蛋白家族的遗传学和生化功能研究被广泛关注,但该基因家族植物中的系统分子进化、表达模式以及功能预测方面的研究仍不完善。本研究通过收集和整理拟南芥和水稻中所有的HSP70基因序列、基因结构和染色体定位等信息,对比分析了拟南芥和水稻中HSP70家族在分子进化方面的差异;同时,从转录水平上初步分析和阐明该家族基因在植物不同组织部位和不同发育时期的表达差异和功能分化。此外,本研究还围绕花药发育的过程,细致分析了HSP70家族基因的表达变化,推测该家族在花药不同时期可能所扮演的角色。本研究为全面理解HSP70家族在单子叶和双子叶植物中的功能异同分化、以及其参与植物生殖发育过程的功能研究提供重要的科学依据。
