The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their mol...The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their molecular masses, isoelectric points (pI), pH-optima and other properties were analyzed by native proteinase gels.It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells.Its N-terminal 1-9 amino acid sequence was determined by Edman degradation, but no homologies to other proteins in the protein data bases were found. 30 and 31 kDa proteinases can be cleaved from the 45, 55 and 65 kDa proteinases to which they are linked. A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed.展开更多
A coupled mathematical model was developed to describe the flow field,temperature distribution of molten steel in the tundish with the channel type induction heating.The molten steel motion was dominated under the com...A coupled mathematical model was developed to describe the flow field,temperature distribution of molten steel in the tundish with the channel type induction heating.The molten steel motion was dominated under the combined effect of the thermal buoyancy and the electro-magnetic forces(EMFs)due to the channel type induction heating.The results indicate that the thermal loss of molten steel in the tundish can be compensated effectively by the channel type induction heating.In addition,the molten steel would flow upward under the thermal buoyancy which can reduce the erosion of refractory.Moreover,the upward flow would increase the residence time of the molten steel in the tundish which provide more opportunities for the inclusion to reach the top surface of the tundish.展开更多
A three-dimensional finite element model of electromagnetic field and temperature field was developed to investigate the effects of induction heat of high frequency electromagnetic field on the early solidification pr...A three-dimensional finite element model of electromagnetic field and temperature field was developed to investigate the effects of induction heat of high frequency electromagnetic field on the early solidification process of molten steel.The results show that the increases of exciting current frequency make the effects of induction heat on solidification increase remarkably.Contrarily,the effects of induction heat on solidification process are weakened sharply with the increase of casting speed.Especially,when the casting speed exceeds a certain value,the effects of induction heat on solidification process are nearly negligible.展开更多
Induction heating has been widely used by the heat treatment industry mainly in the wind-power and automotive sectors,in particular for hardening purposes,in a broad range of applications,its main advantages being the...Induction heating has been widely used by the heat treatment industry mainly in the wind-power and automotive sectors,in particular for hardening purposes,in a broad range of applications,its main advantages being the high repeatability and easy automation of the process,both factors leading to improved manufacturing efficiency and reduced CO;emissions.Though,traditional furnace-based case hardening treatments still represent the choice of reference when performance requirements are particularly demanding,either for the critical operating conditions or safety-related issues.The processes of CIH（Contour Induction Hardening）,compared to the traditional carburizing processes,allows to reduce the deformations after heat treatment.The main purpose of these treatments,as well as increases the surface hardness of the piece,is to induce compressive stresses in the superficial layer,improving the fatigue behavior.A multiphysics magneto-thermal simulation can be developed in order to calculate the temperature distribution in the gear,setting the input parameters such as currents,frequencies and treatment times.展开更多
文摘The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their molecular masses, isoelectric points (pI), pH-optima and other properties were analyzed by native proteinase gels.It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells.Its N-terminal 1-9 amino acid sequence was determined by Edman degradation, but no homologies to other proteins in the protein data bases were found. 30 and 31 kDa proteinases can be cleaved from the 45, 55 and 65 kDa proteinases to which they are linked. A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed.
基金Item Sponsored by National Natural Science Foundation of China[No.50934008]National Natural Science Foundation of China[NO.50904014]
文摘A coupled mathematical model was developed to describe the flow field,temperature distribution of molten steel in the tundish with the channel type induction heating.The molten steel motion was dominated under the combined effect of the thermal buoyancy and the electro-magnetic forces(EMFs)due to the channel type induction heating.The results indicate that the thermal loss of molten steel in the tundish can be compensated effectively by the channel type induction heating.In addition,the molten steel would flow upward under the thermal buoyancy which can reduce the erosion of refractory.Moreover,the upward flow would increase the residence time of the molten steel in the tundish which provide more opportunities for the inclusion to reach the top surface of the tundish.
基金Item Sponsored by College Natural Science Research Project of Jiangsu Province of China(05KJD450043)
文摘A three-dimensional finite element model of electromagnetic field and temperature field was developed to investigate the effects of induction heat of high frequency electromagnetic field on the early solidification process of molten steel.The results show that the increases of exciting current frequency make the effects of induction heat on solidification increase remarkably.Contrarily,the effects of induction heat on solidification process are weakened sharply with the increase of casting speed.Especially,when the casting speed exceeds a certain value,the effects of induction heat on solidification process are nearly negligible.
文摘Induction heating has been widely used by the heat treatment industry mainly in the wind-power and automotive sectors,in particular for hardening purposes,in a broad range of applications,its main advantages being the high repeatability and easy automation of the process,both factors leading to improved manufacturing efficiency and reduced CO;emissions.Though,traditional furnace-based case hardening treatments still represent the choice of reference when performance requirements are particularly demanding,either for the critical operating conditions or safety-related issues.The processes of CIH（Contour Induction Hardening）,compared to the traditional carburizing processes,allows to reduce the deformations after heat treatment.The main purpose of these treatments,as well as increases the surface hardness of the piece,is to induce compressive stresses in the superficial layer,improving the fatigue behavior.A multiphysics magneto-thermal simulation can be developed in order to calculate the temperature distribution in the gear,setting the input parameters such as currents,frequencies and treatment times.
