The propagation and transformation of an elliptic Gaussian optical beam(EGB) passing through a Kerr-law nonlinear conical graded-index rod lens are presented.In the rod lens, the EGB is treated as two dependent optica...The propagation and transformation of an elliptic Gaussian optical beam(EGB) passing through a Kerr-law nonlinear conical graded-index rod lens are presented.In the rod lens, the EGB is treated as two dependent optical beams.The dimensionless beam-width parameters and the inverses of the curvature radii for the wavefront of the two beams are given semi-analytically,and the transformations of the EGB with the rod lens are derived by use of the ABCD law of Gaussian optical beam.展开更多
With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in tradition...With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index （GRIN） probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRiN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube （PMT） to detect fluorescence signals. Experimental results showed that this system using two-photon excitation could implement dynamic fluorescence microendoscopic imaging and monitor the movement of blood flow beneath the skin in anesthetized mice while producing images with higher contrast and signal to noise ratio （SNR） than those using one photon excitation. It would be a useful tool for studying biological processes of small animals or plants in vivo.展开更多
文摘The propagation and transformation of an elliptic Gaussian optical beam(EGB) passing through a Kerr-law nonlinear conical graded-index rod lens are presented.In the rod lens, the EGB is treated as two dependent optical beams.The dimensionless beam-width parameters and the inverses of the curvature radii for the wavefront of the two beams are given semi-analytically,and the transformations of the EGB with the rod lens are derived by use of the ABCD law of Gaussian optical beam.
文摘With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index （GRIN） probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRiN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube （PMT） to detect fluorescence signals. Experimental results showed that this system using two-photon excitation could implement dynamic fluorescence microendoscopic imaging and monitor the movement of blood flow beneath the skin in anesthetized mice while producing images with higher contrast and signal to noise ratio （SNR） than those using one photon excitation. It would be a useful tool for studying biological processes of small animals or plants in vivo.
