A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expre...A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.展开更多
目的初步分析G蛋白α亚基13(GNA13)基因在精神分裂症中的作用,探索精神分裂症发病机制的相关生物通路。方法用出生24 h内的C57BL6N野生型幼鼠进行大脑皮层原代神经元细胞培养,使用重组病毒感染并敲低实验组的Gna13基因,空载病毒感染空...目的初步分析G蛋白α亚基13(GNA13)基因在精神分裂症中的作用,探索精神分裂症发病机制的相关生物通路。方法用出生24 h内的C57BL6N野生型幼鼠进行大脑皮层原代神经元细胞培养,使用重组病毒感染并敲低实验组的Gna13基因,空载病毒感染空载对照组,对照组不做任何干预,在同等条件下继续培养。3组均进行蛋白和mRNA表达水平测定,用高通量测序技术检测其mRNA表达谱,并进行Phenotype表型富集、Kyoto encyclopedia of gene and genomes(KEGG)通路富集、gene ontology(GO)功能富集分析。结果与对照组相比,实验组中存在差异性表达的基因共211个(P<0.05,FDR校正),其中表达上调基因141个,表达下调基因70个。表达下调基因主要与异常有丝分裂、脑形态异常、产前发育迟缓等表型相关,与细胞代谢、细胞周期的催化调节、细胞蛋白质定位等生物过程相关,与细胞周期、代谢途径以及肌动蛋白细胞骨架的调节和粘附力等生物通路相关。结论 GNA13基因可能通过影响神经细胞形态和突触可塑性构成,参与精神分裂症的发生发展。展开更多
文摘A gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco (Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.
文摘目的初步分析G蛋白α亚基13(GNA13)基因在精神分裂症中的作用,探索精神分裂症发病机制的相关生物通路。方法用出生24 h内的C57BL6N野生型幼鼠进行大脑皮层原代神经元细胞培养,使用重组病毒感染并敲低实验组的Gna13基因,空载病毒感染空载对照组,对照组不做任何干预,在同等条件下继续培养。3组均进行蛋白和mRNA表达水平测定,用高通量测序技术检测其mRNA表达谱,并进行Phenotype表型富集、Kyoto encyclopedia of gene and genomes(KEGG)通路富集、gene ontology(GO)功能富集分析。结果与对照组相比,实验组中存在差异性表达的基因共211个(P<0.05,FDR校正),其中表达上调基因141个,表达下调基因70个。表达下调基因主要与异常有丝分裂、脑形态异常、产前发育迟缓等表型相关,与细胞代谢、细胞周期的催化调节、细胞蛋白质定位等生物过程相关,与细胞周期、代谢途径以及肌动蛋白细胞骨架的调节和粘附力等生物通路相关。结论 GNA13基因可能通过影响神经细胞形态和突触可塑性构成,参与精神分裂症的发生发展。
