Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Gibberellic acid （GA） and abscisic acid （ABA） play critical roles in the developmental programs and environmental...Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Gibberellic acid （GA） and abscisic acid （ABA） play critical roles in the developmental programs and environmental responses, respectively, through complex signaling and metabolism networks. However, crosstalk between the two phytohormones in stress responses remains largely unknown. In this study, we report that GIBBERELLIN-INSENSITIVE DWARF 1 （GID1）, a soluble receptor for GA, regulates stomata[ develop- ment and patterning in rice （Oryza sativa L.）. The gidl mutant showed impaired biosynthesis of endogenous ABA under drought stress conditions, but it exhibited enhanced sensi- tivity to exogenous ABA. Scanning electron microscope and infrared thermal image analysis indicated an increase in the stomatal conductance in the gidl mutant under drought conditions. Interestingly, the gidl mutant had increased levels of chlorophyll and carbohydrates under submergence conditions, and showed enhanced reactive oxygen species （ROS）-scavenging ability and submergence tolerance compared with the wild-type. Further analyses suggested that the function of GID1 in submergence responses is partially dependent on ABA, and GA signaling by GID1 is involved in submergence tolerance by modulating carbohydrate consumption. Taken together, these findings suggest GID1 plays distinct roles in stomatal response and submergence tolerance through both the ABA and GA signaling pathways in rice.展开更多
基金supported by grants from the National Program for Basic Research of China(2012CB114305)the National Program on High Technology Development(2012AA10A303)+1 种基金the National Natural Science Foundation of China(31271316 and J1103510 to Chang Y and Huang F)the National Program of China for Transgenic Research(2011ZX08009-003002,2011ZX08001-003)
文摘Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Gibberellic acid （GA） and abscisic acid （ABA） play critical roles in the developmental programs and environmental responses, respectively, through complex signaling and metabolism networks. However, crosstalk between the two phytohormones in stress responses remains largely unknown. In this study, we report that GIBBERELLIN-INSENSITIVE DWARF 1 （GID1）, a soluble receptor for GA, regulates stomata[ develop- ment and patterning in rice （Oryza sativa L.）. The gidl mutant showed impaired biosynthesis of endogenous ABA under drought stress conditions, but it exhibited enhanced sensi- tivity to exogenous ABA. Scanning electron microscope and infrared thermal image analysis indicated an increase in the stomatal conductance in the gidl mutant under drought conditions. Interestingly, the gidl mutant had increased levels of chlorophyll and carbohydrates under submergence conditions, and showed enhanced reactive oxygen species （ROS）-scavenging ability and submergence tolerance compared with the wild-type. Further analyses suggested that the function of GID1 in submergence responses is partially dependent on ABA, and GA signaling by GID1 is involved in submergence tolerance by modulating carbohydrate consumption. Taken together, these findings suggest GID1 plays distinct roles in stomatal response and submergence tolerance through both the ABA and GA signaling pathways in rice.
