Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years.Herein,we isolated a new mammalian orthoreovirus(MRV),Pika/MRV/GCCDC7/2019(PMR...Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years.Herein,we isolated a new mammalian orthoreovirus(MRV),Pika/MRV/GCCDC7/2019(PMRV-GCCDC7),in the Qinghai-Tibet Plateau wild pika(Ochotona curzoniae).Though the PMRVGCCDC7 shows features of a typical reovirus with ten gene segments arranged in 3:3:4 in length,the virus belongs to an independent evolutionary branch compared to other MRVs based on phylogenetic tree analysis.The results of cellular susceptibility,species tropism,and replication kinetics of PMRV-GCCDC7 indicated the virus could infect four human cell lines(A549,Huh7,HCT,and LoVo)and six non-human cell lines,including Vero-E6,LLCMK2,BHK-21,N2a,MDCK,and RfKT cell,derived from diverse mammals,i.e.monkey,mice,canine and bat,which revealed the potential of PMRV-GCCDC7 to infect a variety of hosts.Infection of BALB/c mice with PMRVGCCDC7 via intranasal inoculation led to relative weight loss,lung tissue damage and inflammation with the increase of virus titer,but no serious respiratory symptoms and death occurred.The characterization of the new reovirus from a plateau-based wild animal has expanded our knowledge of the host range of MRV and provided insight into its risk of trans-species transmission and zoonotic diseases.展开更多
Omicron(B.1.1.529),the fifth variant of concern(VOC)of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2),was firstly identified in November 2021 in South Africa.Omicron contains far more genome mutations t...Omicron(B.1.1.529),the fifth variant of concern(VOC)of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2),was firstly identified in November 2021 in South Africa.Omicron contains far more genome mutations than any other VOCs ever found,raising significant concerns about its increased transmissibility and immune evasion.Here,we report the importation of the Omicron variant into Beijing,China,in December 2021.Full‐length genome sequences of five imported strains were obtained,with their genetic features characterized.Each strain contained 57 to 61 nucleotide substitutions,39 deletions,and 9 insertions in the genome.Thirty to thirty‐two amino acid changes were found in the spike proteins of the five strains.The phylogenetic tree constructed by the maximum likelihood method showed that all five imported genomes belonged to Omicron(BA.1)(alias of B.1.1.529.1),which is leading to the current surge of coronavirus disease 2019(COVID‐19)cases worldwide.The globally increased COVID‐19 cases driven by the Omicron variant pose a significant challenge to disease prevention and control in China.Continuous viral genetic surveillance and increased testing among international travellers are required to contain this highly contagious variant.展开更多
基金the National Key Research and Development Program of China(2021YFC0863400,2022YFC2604105).
文摘Emerging and re-emerging viruses from wild animals have seriously threatened the health of humans and domesticated animals in recent years.Herein,we isolated a new mammalian orthoreovirus(MRV),Pika/MRV/GCCDC7/2019(PMRV-GCCDC7),in the Qinghai-Tibet Plateau wild pika(Ochotona curzoniae).Though the PMRVGCCDC7 shows features of a typical reovirus with ten gene segments arranged in 3:3:4 in length,the virus belongs to an independent evolutionary branch compared to other MRVs based on phylogenetic tree analysis.The results of cellular susceptibility,species tropism,and replication kinetics of PMRV-GCCDC7 indicated the virus could infect four human cell lines(A549,Huh7,HCT,and LoVo)and six non-human cell lines,including Vero-E6,LLCMK2,BHK-21,N2a,MDCK,and RfKT cell,derived from diverse mammals,i.e.monkey,mice,canine and bat,which revealed the potential of PMRV-GCCDC7 to infect a variety of hosts.Infection of BALB/c mice with PMRVGCCDC7 via intranasal inoculation led to relative weight loss,lung tissue damage and inflammation with the increase of virus titer,but no serious respiratory symptoms and death occurred.The characterization of the new reovirus from a plateau-based wild animal has expanded our knowledge of the host range of MRV and provided insight into its risk of trans-species transmission and zoonotic diseases.
基金supported by the Capital's Funds for Health Improvement and Research(Project No.2021-1G-3012,2022-2G-30115)the Beijing Science and Technology Planning Project of Beijing Science and Technology Commission(Project No.Z211100002521019).
文摘Omicron(B.1.1.529),the fifth variant of concern(VOC)of severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2),was firstly identified in November 2021 in South Africa.Omicron contains far more genome mutations than any other VOCs ever found,raising significant concerns about its increased transmissibility and immune evasion.Here,we report the importation of the Omicron variant into Beijing,China,in December 2021.Full‐length genome sequences of five imported strains were obtained,with their genetic features characterized.Each strain contained 57 to 61 nucleotide substitutions,39 deletions,and 9 insertions in the genome.Thirty to thirty‐two amino acid changes were found in the spike proteins of the five strains.The phylogenetic tree constructed by the maximum likelihood method showed that all five imported genomes belonged to Omicron(BA.1)(alias of B.1.1.529.1),which is leading to the current surge of coronavirus disease 2019(COVID‐19)cases worldwide.The globally increased COVID‐19 cases driven by the Omicron variant pose a significant challenge to disease prevention and control in China.Continuous viral genetic surveillance and increased testing among international travellers are required to contain this highly contagious variant.
