microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesen...microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs in bone marrow-derived mesen- chymal stem cells, neural stem cells and neurons, miR-124 expression was substantially reduced in bone marrow-derived mesenchymal stem cells compared with the other cell types. We con- structed a lentiviral vector overexpressing miR-124 and transfected it into bone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markers [3-III tu- bulin and microtubule-associated protein-2 were significantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These re- sults suggest that miR-124 plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells into neurons. Our findings should facilitate the development of novel strategies for enhancing the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for spinal cord injury.展开更多
目的:揭示肝癌诱发过程中肝组织基因表达谱的演变,为中医药防治原发性肝癌提供参考。方法:采用DEN诱发大鼠肝癌,分别于诱癌的第4周、8周、16周、20周(肝癌形成)切取肝(合肝癌)组织,常规提取RNA,Affymetrix Rat 230A GeneChip及技术检测...目的:揭示肝癌诱发过程中肝组织基因表达谱的演变,为中医药防治原发性肝癌提供参考。方法:采用DEN诱发大鼠肝癌,分别于诱癌的第4周、8周、16周、20周(肝癌形成)切取肝(合肝癌)组织,常规提取RNA,Affymetrix Rat 230A GeneChip及技术检测大鼠肝组织基因表达的差异和演变。结果:在芯片的15710个基因中,正常组有9225个基因表达,诱癌4周表达增至9396个,8周增至9872个,16周增至10496个,20周有10420个。在肝癌诱发过程中,存在大量基因表达的消长以及高表达基因数的增加,其中部分已知基因的结果。结论:DEN诱发大鼠肝癌过程中基因组变化是十分复杂的,而且文献追踪表明,国内外对在这些基因的功能及其与肝癌发生、发展的关系大多不明确。因此,如何逐一找到那些起着关键作用的基因,进一步阐释其在肝癌的发生、发展和转归中的作用,及其与中医证候演变和相应治法的关系,是今后中医基础理论实验研究的重要方向。展开更多
基金supported by the National Natural Science Foundation of China,No.81070971
文摘microRNAs （miRNAs） play an important regulatory role in the self-renewal and differentiation of stem cells. In this study, we examined the effects of miRNA-124 （miR-124） overexpression in bone marrow-derived mesenchymal stem cells. In particular, we focused on the effect of overexpression on the differentiation of bone marrow-derived mesenchymal stem cells into neurons. First, we used GeneChip technology to analyze the expression of miRNAs in bone marrow-derived mesen- chymal stem cells, neural stem cells and neurons, miR-124 expression was substantially reduced in bone marrow-derived mesenchymal stem cells compared with the other cell types. We con- structed a lentiviral vector overexpressing miR-124 and transfected it into bone marrow-derived mesenchymal stem cells. Intracellular expression levels of the neuronal early markers [3-III tu- bulin and microtubule-associated protein-2 were significantly increased, and apoptosis induced by oxygen and glucose deprivation was reduced in transfected cells. After miR-124-transfected bone marrow-derived mesenchymal stem cells were transplanted into the injured rat spinal cord, a large number of cells positive for the neuronal marker neurofilament-200 were observed in the transplanted region. The Basso-Beattie-Bresnahan locomotion scores showed that the motor function of the hind limb of rats with spinal cord injury was substantially improved. These re- sults suggest that miR-124 plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells into neurons. Our findings should facilitate the development of novel strategies for enhancing the therapeutic efficacy of bone marrow-derived mesenchymal stem cell transplantation for spinal cord injury.
文摘目的:揭示肝癌诱发过程中肝组织基因表达谱的演变,为中医药防治原发性肝癌提供参考。方法:采用DEN诱发大鼠肝癌,分别于诱癌的第4周、8周、16周、20周(肝癌形成)切取肝(合肝癌)组织,常规提取RNA,Affymetrix Rat 230A GeneChip及技术检测大鼠肝组织基因表达的差异和演变。结果:在芯片的15710个基因中,正常组有9225个基因表达,诱癌4周表达增至9396个,8周增至9872个,16周增至10496个,20周有10420个。在肝癌诱发过程中,存在大量基因表达的消长以及高表达基因数的增加,其中部分已知基因的结果。结论:DEN诱发大鼠肝癌过程中基因组变化是十分复杂的,而且文献追踪表明,国内外对在这些基因的功能及其与肝癌发生、发展的关系大多不明确。因此,如何逐一找到那些起着关键作用的基因,进一步阐释其在肝癌的发生、发展和转归中的作用,及其与中医证候演变和相应治法的关系,是今后中医基础理论实验研究的重要方向。
